Restriction map for
pF1KE0285
(Linear) (Six-base) MAPSORT of: KIEE0285.seq Check: 3292 from: 1 to: 663
KIEE0285.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AatII G_ACGT'C
Cuts at: 0 242 663
Size: 242 421
AccI GT'mk_AC
Cuts at: 0 156 663
Size: 156 507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 663
Size: 97 566
AleI CACnn'nnGTG
Cuts at: 0 64 663
Size: 64 599
ApaI G_GGCC'C
Cuts at: 0 656 663
Size: 656 7
BamHI G'GATC_C
Cuts at: 0 112 663
Size: 112 551
BanII G_rGCy'C
Cuts at: 0 656 663
Size: 656 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 85 352 663
Size: 85 267 311
Fragments arranged by size:
311 267 85
BbvCI CC'TCA_GC
Cuts at: 0 169 663
Size: 169 494
BciVI GTATCCnnnnn_n'
Cuts at: 0 507 663
Size: 507 156
Bme1580I G_kGCm'C
Cuts at: 0 656 663
Size: 656 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 465 640 663
Size: 465 175 23
Fragments arranged by size:
465 175 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 663
Size: 159 504
Bpu10I CC'TnA_GC
Cuts at: 0 169 663
Size: 169 494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 663
Size: 527 136
BsaI GGTCTCn'nnnn_
Cuts at: 0 98 663
Size: 98 565
BsaHI Gr'CG_yC
Cuts at: 0 239 663
Size: 239 424
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 663
Size: 10 653
BseYI C'CCAG_C
Cuts at: 0 36 663
Size: 36 627
BsiHKAI G_wGCw'C
Cuts at: 0 543 663
Size: 543 120
Bsp1286I G_dGCh'C
Cuts at: 0 543 656 663
Size: 543 113 7
Fragments arranged by size:
543 113 7
BspHI T'CATG_A
Cuts at: 0 321 663
Size: 321 342
BsrGI T'GTAC_A
Cuts at: 0 123 663
Size: 123 540
BstAPI GCAn_nnn'nTGC
Cuts at: 0 387 663
Size: 387 276
BstEII G'GTnAC_C
Cuts at: 0 610 663
Size: 610 53
BstYI r'GATC_y
Cuts at: 0 112 663
Size: 112 551
BtgI C'CryG_G
Cuts at: 0 116 663
Size: 116 547
DraIII CAC_nnn'GTG
Cuts at: 0 648 663
Size: 648 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 159 663
Size: 97 62 504
Fragments arranged by size:
504 97 62
EcoO109I rG'GnC_Cy
Cuts at: 0 652 653 663
Size: 652 1 10
Fragments arranged by size:
652 10 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 102 221 663
Size: 102 119 442
Fragments arranged by size:
442 119 102
FspI TGC'GCA
Cuts at: 0 380 663
Size: 380 283
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 269 301 663
Size: 269 32 362
Fragments arranged by size:
362 269 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 663
Size: 170 493
MslI CAynn'nnrTG
Cuts at: 0 64 181 663
Size: 64 117 482
Fragments arranged by size:
482 117 64
NcoI C'CATG_G
Cuts at: 0 116 663
Size: 116 547
NspI r_CATG'y
Cuts at: 0 262 663
Size: 262 401
PflMI CCAn_nnn'nTGG
Cuts at: 0 648 663
Size: 648 15
PspOMI G'GGCC_C
Cuts at: 0 652 663
Size: 652 11
PstI C_TGCA'G
Cuts at: 0 554 663
Size: 554 109
SfcI C'TryA_G
Cuts at: 0 529 550 582 663
Size: 529 21 32 81
Fragments arranged by size:
529 81 32 21
SmlI C'TyrA_G
Cuts at: 0 542 663
Size: 542 121
StyI C'CwwG_G
Cuts at: 0 116 663
Size: 116 547
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 659 663
Size: 659 4
TatI w'GTAC_w
Cuts at: 0 123 663
Size: 123 540
ZraI GAC'GTC
Cuts at: 0 240 663
Size: 240 423
Enzymes that do cut:
AatII AccI AcuI AleI ApaI BamHI BanII BbsI
BbvCI BciVI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BseRI BseYI BsiHKAI Bsp1286I BspHI BsrGI BstAPI
BstEII BstYI BtgI DraIII Eco57MI EcoO109I FalI FspI
Hin4I MmeI MslI NcoI NspI PflMI PspOMI PstI
SfcI SmlI StyI TaqII TatI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI
BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI