(Linear) (Six-base) MAPSORT of: KIEE0273.seq  Check: 5031  from: 1  to: 309



KIEE0273.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

BanI G'GyrC_C

Cuts at:      0      43     309 
   Size:         43     266
 
BbeI G_GCGC'C

Cuts at:      0      47     309 
   Size:         47     262
 
BglI GCCn_nnn'nGGC

Cuts at:      0     125     309 
   Size:        125     184
 
BmgBI CAC'GTC

Cuts at:      0     258     309 
   Size:        258      51
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     309 
   Size:        256      53
 
BsaHI Gr'CG_yC

Cuts at:      0      44     128     309 
   Size:         44      84     181

  Fragments arranged by size: 

                 181      84      44
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     258     309 
   Size:        258      51
 
BtgI C'CryG_G

Cuts at:      0       9     120     251     309 
   Size:          9     111     131      58

  Fragments arranged by size: 

                 131     111      58       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     218     309 
   Size:        218      91
 
EaeI y'GGCC_r

Cuts at:      0       6     309 
   Size:          6     303
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      35      94     309 
   Size:         35      59     215

  Fragments arranged by size: 

                 215      59      35
 
HaeII r_GCGC'y

Cuts at:      0      47     279     309 
   Size:         47     232      30

  Fragments arranged by size: 

                 232      47      30
 
KasI G'GCGC_C

Cuts at:      0      43     309 
   Size:         43     266
 
MslI CAynn'nnrTG

Cuts at:      0     250     256     309 
   Size:        250       6      53

  Fragments arranged by size: 

                 250      53       6
 
MspA1I CmG'CkG

Cuts at:      0      11     122     309 
   Size:         11     111     187

  Fragments arranged by size: 

                 187     111      11
 
NarI GG'CG_CC

Cuts at:      0      44     309 
   Size:         44     265
 
NcoI C'CATG_G

Cuts at:      0     251     309 
   Size:        251      58
 
PfoI T'CCnGG_A

Cuts at:      0     185     309 
   Size:        185     124
 
SacII CC_GC'GG

Cuts at:      0      12     123     309 
   Size:         12     111     186

  Fragments arranged by size: 

                 186     111      12
 
SfoI GGC'GCC

Cuts at:      0      45     309 
   Size:         45     264
 
SmlI C'TyrA_G

Cuts at:      0     271     309 
   Size:        271      38
 
StyI C'CwwG_G

Cuts at:      0      81     251     309 
   Size:         81     170      58

  Fragments arranged by size: 

                 170      81      58
 
XmnI GAAnn'nnTTC

Cuts at:      0     181     309 
   Size:        181     128
 

 Enzymes that do cut:

     BanI     BbeI     BglI    BmgBI    BpuEI    BsaHI    BstXI     BtgI
    BtgZI     EaeI     EciI    HaeII     KasI     MslI   MspA1I     NarI
     NcoI     PfoI    SacII     SfoI     SmlI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI