Restriction map for
pF1KE0272
(Linear) (Six-base) MAPSORT of: KIEE0272.seq Check: 9573 from: 1 to: 627
KIEE0272.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 72 627
Size: 72 555
AleI CACnn'nnGTG
Cuts at: 0 568 627
Size: 568 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 400 627
Size: 400 227
ApaI G_GGCC'C
Cuts at: 0 589 627
Size: 589 38
AvaI C'yCGr_G
Cuts at: 0 5 549 627
Size: 5 544 78
Fragments arranged by size:
544 78 5
BanI G'GyrC_C
Cuts at: 0 619 627
Size: 619 8
BanII G_rGCy'C
Cuts at: 0 173 589 627
Size: 173 416 38
Fragments arranged by size:
416 173 38
Bme1580I G_kGCm'C
Cuts at: 0 133 575 589 624 627
Size: 133 442 14 35 3
Fragments arranged by size:
442 133 35 14 3
BmgBI CAC'GTC
Cuts at: 0 297 627
Size: 297 330
BmrI ACTGGGnnnn_n'
Cuts at: 0 382 627
Size: 382 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 551 627
Size: 168 383 76
Fragments arranged by size:
383 168 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 553 627
Size: 553 74
BsaAI yAC'GTr
Cuts at: 0 165 627
Size: 165 462
BsaWI w'CCGG_w
Cuts at: 0 227 242 627
Size: 227 15 385
Fragments arranged by size:
385 227 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 627
Size: 400 30 197
Fragments arranged by size:
400 197 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 416 627
Size: 416 211
BseYI C'CCAG_C
Cuts at: 0 90 260 529 627
Size: 90 170 269 98
Fragments arranged by size:
269 170 98 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 627
Size: 382 245
Bsp1286I G_dGCh'C
Cuts at: 0 133 173 575 589 624 627
Size: 133 40 402 14 35 3
Fragments arranged by size:
402 133 40 35 14 3
BspEI T'CCGG_A
Cuts at: 0 242 627
Size: 242 385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 72 627
Size: 72 555
BsrBI CCG'CTC
Cuts at: 0 48 57 66 627
Size: 48 9 9 561
Fragments arranged by size:
561 48 9 9
BsrGI T'GTAC_A
Cuts at: 0 330 443 627
Size: 330 113 184
Fragments arranged by size:
330 184 113
BtgI C'CryG_G
Cuts at: 0 24 419 627
Size: 24 395 208
Fragments arranged by size:
395 208 24
DrdI GACnn_nn'nnGTC
Cuts at: 0 301 627
Size: 301 326
EaeI y'GGCC_r
Cuts at: 0 17 488 627
Size: 17 471 139
Fragments arranged by size:
471 139 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 551 627
Size: 168 383 76
Fragments arranged by size:
383 168 76
EcoO109I rG'GnC_Cy
Cuts at: 0 545 586 627
Size: 545 41 41
Fragments arranged by size:
545 41 41
HaeII r_GCGC'y
Cuts at: 0 91 627
Size: 91 536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 400 432 500 532 627
Size: 400 32 68 32 95
Fragments arranged by size:
400 95 68 32 32
MscI TGG'CCA
Cuts at: 0 490 627
Size: 490 137
MslI CAynn'nnrTG
Cuts at: 0 310 568 627
Size: 310 258 59
Fragments arranged by size:
310 258 59
MspA1I CmG'CkG
Cuts at: 0 15 26 75 110 146 217 627
Size: 15 11 49 35 36 71 410
Fragments arranged by size:
410 71 49 36 35 15 11
NcoI C'CATG_G
Cuts at: 0 419 627
Size: 419 208
NspI r_CATG'y
Cuts at: 0 381 627
Size: 381 246
PmlI CAC'GTG
Cuts at: 0 165 627
Size: 165 462
PspOMI G'GGCC_C
Cuts at: 0 585 627
Size: 585 42
PvuII CAG'CTG
Cuts at: 0 110 217 627
Size: 110 107 410
Fragments arranged by size:
410 110 107
SacII CC_GC'GG
Cuts at: 0 27 627
Size: 27 600
SfcI C'TryA_G
Cuts at: 0 351 435 627
Size: 351 84 192
Fragments arranged by size:
351 192 84
SmaI CCC'GGG
Cuts at: 0 7 627
Size: 7 620
SphI G_CATG'C
Cuts at: 0 381 627
Size: 381 246
StyI C'CwwG_G
Cuts at: 0 419 540 627
Size: 419 121 87
Fragments arranged by size:
419 121 87
TatI w'GTAC_w
Cuts at: 0 330 443 627
Size: 330 113 184
Fragments arranged by size:
330 184 113
XmaI C'CCGG_G
Cuts at: 0 5 627
Size: 5 622
Enzymes that do cut:
AarI AleI AlfI ApaI AvaI BanI BanII Bme1580I
BmgBI BmrI BpmI BsaI BsaAI BsaWI BsaXI BseRI
BseYI BsgI Bsp1286I BspEI BspMI BsrBI BsrGI BtgI
DrdI EaeI Eco57MI EcoO109I HaeII Hin4I MscI MslI
MspA1I NcoI NspI PmlI PspOMI PvuII SacII SfcI
SmaI SphI StyI TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmtI BplI
Bpu10I BpuEI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspHI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII EagI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmnI ZraI