(Linear) (Six-base) MAPSORT of: KIEE0265.seq  Check: 5952  from: 1  to: 390



KIEE0265.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AccI GT'mk_AC

Cuts at:      0     149     390 
   Size:        149     241
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     390 
   Size:         55     335
 
AhdI GACnn_n'nnGTC

Cuts at:      0     365     390 
   Size:        365      25
 
AscI GG'CGCG_CC

Cuts at:      0     140     390 
   Size:        140     250
 
AvaI C'yCGr_G

Cuts at:      0     166     212     390 
   Size:        166      46     178

  Fragments arranged by size: 

                 178     166      46
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     232     364     390 
   Size:        232     132      26

  Fragments arranged by size: 

                 232     132      26
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     235     390 
   Size:        211      24     155

  Fragments arranged by size: 

                 211     155      24
 
BsaHI Gr'CG_yC

Cuts at:      0     320     390 
   Size:        320      70
 
BseYI C'CCAG_C

Cuts at:      0     291     390 
   Size:        291      99
 
BsrFI r'CCGG_y

Cuts at:      0     136     390 
   Size:        136     254
 
BssHII G'CGCG_C

Cuts at:      0     140     390 
   Size:        140     250
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      99     390 
   Size:         99     291
 
BstYI r'GATC_y

Cuts at:      0     185     390 
   Size:        185     205
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      79     291     390 
   Size:         79     212      99

  Fragments arranged by size: 

                 212      99      79
 
EaeI y'GGCC_r

Cuts at:      0     257     390 
   Size:        257     133
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     211     235     390 
   Size:         55     156      24     155

  Fragments arranged by size: 

                 156     155      55      24
 
FspI TGC'GCA

Cuts at:      0     106     390 
   Size:        106     284
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     351     383     390 
   Size:        351      32       7

  Fragments arranged by size: 

                 351      32       7
 
MscI TGG'CCA

Cuts at:      0     259     390 
   Size:        259     131
 
MspA1I CmG'CkG

Cuts at:      0     255     390 
   Size:        255     135
 
NaeI GCC'GGC

Cuts at:      0     138     390 
   Size:        138     252
 
NgoMIV G'CCGG_C

Cuts at:      0     136     390 
   Size:        136     254
 
PasI CC'CwG_GG

Cuts at:      0     312     390 
   Size:        312      78
 
PfoI T'CCnGG_A

Cuts at:      0     188     390 
   Size:        188     202
 
PspXI vC'TCGA_Gb

Cuts at:      0     166     390 
   Size:        166     224
 
PvuII CAG'CTG

Cuts at:      0     255     390 
   Size:        255     135
 
SmlI C'TyrA_G

Cuts at:      0     166     390 
   Size:        166     224
 
StyI C'CwwG_G

Cuts at:      0      38     390 
   Size:         38     352
 
XhoI C'TCGA_G

Cuts at:      0     166     390 
   Size:        166     224
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AscI     AvaI     BbsI     BpmI    BsaHI
    BseYI    BsrFI   BssHII   BstAPI    BstYI    BtgZI     EaeI  Eco57MI
     FspI    Hin4I     MscI   MspA1I     NaeI   NgoMIV     PasI     PfoI
    PspXI    PvuII     SmlI     StyI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI