Restriction map for
pF1KE0255
(Linear) (Six-base) MAPSORT of: KIEE0255.seq Check: 9836 from: 1 to: 1266
KIEE0255.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 846 1266
Size: 846 420
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 1106 1266
Size: 353 753 160
Fragments arranged by size:
753 353 160
AflIII A'CryG_T
Cuts at: 0 605 1266
Size: 605 661
AlwNI CAG_nnn'CTG
Cuts at: 0 517 885 1266
Size: 517 368 381
Fragments arranged by size:
517 381 368
ApaI G_GGCC'C
Cuts at: 0 273 1266
Size: 273 993
AseI AT'TA_AT
Cuts at: 0 539 1266
Size: 539 727
AvaI C'yCGr_G
Cuts at: 0 840 1266
Size: 840 426
BamHI G'GATC_C
Cuts at: 0 35 1266
Size: 35 1231
BanII G_rGCy'C
Cuts at: 0 273 1266
Size: 273 993
BbsI GAAGACnn'nnnn_
Cuts at: 0 977 1266
Size: 977 289
BbvCI CC'TCA_GC
Cuts at: 0 285 1266
Size: 285 981
Bme1580I G_kGCm'C
Cuts at: 0 273 1266
Size: 273 993
BmrI ACTGGGnnnn_n'
Cuts at: 0 34 536 908 1046 1249 1266
Size: 34 502 372 138 203 17
Fragments arranged by size:
502 372 203 138 34 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 731 1266
Size: 731 535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 703 775 1266
Size: 319 384 72 491
Fragments arranged by size:
491 384 319 72
Bpu10I CC'TnA_GC
Cuts at: 0 285 1266
Size: 285 981
BsaI GGTCTCn'nnnn_
Cuts at: 0 997 1266
Size: 997 269
BsaHI Gr'CG_yC
Cuts at: 0 203 1266
Size: 203 1063
BsaWI w'CCGG_w
Cuts at: 0 356 1266
Size: 356 910
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 355 1120 1266
Size: 355 765 146
Fragments arranged by size:
765 355 146
BseYI C'CCAG_C
Cuts at: 0 385 1136 1266
Size: 385 751 130
Fragments arranged by size:
751 385 130
Bsp1286I G_dGCh'C
Cuts at: 0 273 1266
Size: 273 993
BspEI T'CCGG_A
Cuts at: 0 356 1266
Size: 356 910
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 846 1266
Size: 846 420
BstEII G'GTnAC_C
Cuts at: 0 1212 1266
Size: 1212 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 921 1266
Size: 249 672 345
Fragments arranged by size:
672 345 249
BstYI r'GATC_y
Cuts at: 0 35 1266
Size: 35 1231
BtgI C'CryG_G
Cuts at: 0 72 1266
Size: 72 1194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1093 1266
Size: 1093 173
EarI CTCTTCn'nnn_
Cuts at: 0 1237 1266
Size: 1237 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 353 703 775 1106 1266
Size: 319 34 350 72 331 160
Fragments arranged by size:
350 331 319 160 72 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1207 1266
Size: 1207 59
EcoO109I rG'GnC_Cy
Cuts at: 0 94 269 303 723 1266
Size: 94 175 34 420 543
Fragments arranged by size:
543 420 175 94 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 994 1266
Size: 994 272
HaeII r_GCGC'y
Cuts at: 0 823 1266
Size: 823 443
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 302 334 1266
Size: 302 32 932
Fragments arranged by size:
932 302 32
HincII GTy'rAC
Cuts at: 0 1251 1266
Size: 1251 15
MfeI C'AATT_G
Cuts at: 0 901 1266
Size: 901 365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1266
Size: 902 364
MslI CAynn'nnrTG
Cuts at: 0 610 1266
Size: 610 656
NcoI C'CATG_G
Cuts at: 0 72 1266
Size: 72 1194
NspI r_CATG'y
Cuts at: 0 1174 1266
Size: 1174 92
PflMI CCAn_nnn'nTGG
Cuts at: 0 774 1266
Size: 774 492
PfoI T'CCnGG_A
Cuts at: 0 296 587 1266
Size: 296 291 679
Fragments arranged by size:
679 296 291
PpuMI rG'GwC_Cy
Cuts at: 0 723 1266
Size: 723 543
PspOMI G'GGCC_C
Cuts at: 0 269 1266
Size: 269 997
PstI C_TGCA'G
Cuts at: 0 92 875 1266
Size: 92 783 391
Fragments arranged by size:
783 391 92
SfcI C'TryA_G
Cuts at: 0 88 871 1266
Size: 88 783 395
Fragments arranged by size:
783 395 88
SmaI CCC'GGG
Cuts at: 0 842 1266
Size: 842 424
StuI AGG'CCT
Cuts at: 0 1127 1188 1266
Size: 1127 61 78
Fragments arranged by size:
1127 78 61
StyI C'CwwG_G
Cuts at: 0 72 815 937 1061 1266
Size: 72 743 122 124 205
Fragments arranged by size:
743 205 124 122 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 168 1120 1234 1266
Size: 168 952 114 32
Fragments arranged by size:
952 168 114 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 14 1266
Size: 14 1252
XmaI C'CCGG_G
Cuts at: 0 840 1266
Size: 840 426
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI AseI AvaI BamHI
BanII BbsI BbvCI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaHI BsaWI BseRI BseYI Bsp1286I BspEI BspMI
BstEII BstXI BstYI BtgI BtgZI EarI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII MfeI MmeI MslI
NcoI NspI PflMI PfoI PpuMI PspOMI PstI SfcI
SmaI StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AsiSI AvrII
BaeI BanI BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BpuEI BsaAI BsaBI BsaXI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XbaI XhoI XmnI ZraI