(Linear) (Six-base) MAPSORT of: KIEE0251.seq  Check: 2515  from: 1  to: 267



KIEE0251.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      72     140     267 
   Size:         72      68     127

  Fragments arranged by size: 

                 127      72      68
 
ApaI G_GGCC'C

Cuts at:      0     122     267 
   Size:        122     145
 
BanII G_rGCy'C

Cuts at:      0      61     122     193     235     267 
   Size:         61      61      71      42      32

  Fragments arranged by size: 

                  71      61      61      42      32
 
Bme1580I G_kGCm'C

Cuts at:      0      49      99     122     267 
   Size:         49      50      23     145

  Fragments arranged by size: 

                 145      50      49      23
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62      72     219     267 
   Size:         62      10     147      48

  Fragments arranged by size: 

                 147      62      48      10
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     173     267 
   Size:        173      94
 
BseYI C'CCAG_C

Cuts at:      0      60     121     267 
   Size:         60      61     146

  Fragments arranged by size: 

                 146      61      60
 
BsiHKAI G_wGCw'C

Cuts at:      0     193     235     267 
   Size:        193      42      32

  Fragments arranged by size: 

                 193      42      32
 
Bsp1286I G_dGCh'C

Cuts at:      0      49      61      99     122     193     235     267 
   Size:         49      12      38      23      71      42      32

  Fragments arranged by size: 

                  71      49      42      38      32      23      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     155     267 
   Size:        155     112
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     140     267 
   Size:        140     127
 
BtsI GCAGTG_nn'

Cuts at:      0      31     267 
   Size:         31     236
 
EaeI y'GGCC_r

Cuts at:      0     243     267 
   Size:        243      24
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      26     267 
   Size:         26     241
 
EcoICRI GAG'CTC

Cuts at:      0     191     233     267 
   Size:        191      42      34

  Fragments arranged by size: 

                 191      42      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62      72     219     267 
   Size:         62      10     147      48

  Fragments arranged by size: 

                 147      62      48      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     204     267 
   Size:        204      63
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     267 
   Size:        218      49
 
MscI TGG'CCA

Cuts at:      0     245     267 
   Size:        245      22
 
MspA1I CmG'CkG

Cuts at:      0      92     137     239     267 
   Size:         92      45     102      28

  Fragments arranged by size: 

                 102      92      45      28
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     106     267 
   Size:        106     161
 
PspOMI G'GGCC_C

Cuts at:      0     118     267 
   Size:        118     149
 
PvuII CAG'CTG

Cuts at:      0      92     137     239     267 
   Size:         92      45     102      28

  Fragments arranged by size: 

                 102      92      45      28
 
SacI G_AGCT'C

Cuts at:      0     193     235     267 
   Size:        193      42      32

  Fragments arranged by size: 

                 193      42      32
 

 Enzymes that do cut:

    AlwNI     ApaI    BanII Bme1580I     BpmI     BsaI    BseYI  BsiHKAI
 Bsp1286I    BspMI   BstAPI     BtsI     EaeI     EciI  EcoICRI  Eco57MI
    EcoNI     MmeI     MscI   MspA1I    PflMI   PspOMI    PvuII     SacI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI