Restriction map for
pF1KE0245
(Linear) (Six-base) MAPSORT of: KIEE0245.seq Check: 2862 from: 1 to: 720
KIEE0245.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
Acc65I G'GTAC_C
Cuts at: 0 688 720
Size: 688 32
ApaI G_GGCC'C
Cuts at: 0 396 720
Size: 396 324
AvaI C'yCGr_G
Cuts at: 0 107 520 581 720
Size: 107 413 61 139
Fragments arranged by size:
413 139 107 61
BanI G'GyrC_C
Cuts at: 0 688 720
Size: 688 32
BanII G_rGCy'C
Cuts at: 0 396 720
Size: 396 324
BbvCI CC'TCA_GC
Cuts at: 0 376 720
Size: 376 344
BglI GCCn_nnn'nGGC
Cuts at: 0 151 467 720
Size: 151 316 253
Fragments arranged by size:
316 253 151
Bme1580I G_kGCm'C
Cuts at: 0 396 720
Size: 396 324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 550 646 720
Size: 291 259 96 74
Fragments arranged by size:
291 259 96 74
Bpu10I CC'TnA_GC
Cuts at: 0 376 720
Size: 376 344
BsaI GGTCTCn'nnnn_
Cuts at: 0 127 269 720
Size: 127 142 451
Fragments arranged by size:
451 142 127
BsaHI Gr'CG_yC
Cuts at: 0 189 720
Size: 189 531
BsaWI w'CCGG_w
Cuts at: 0 83 720
Size: 83 637
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 188 218 415 445 720
Size: 188 30 197 30 275
Fragments arranged by size:
275 197 188 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 538 720
Size: 19 519 182
Fragments arranged by size:
519 182 19
BseYI C'CCAG_C
Cuts at: 0 69 279 351 444 465 631 720
Size: 69 210 72 93 21 166 89
Fragments arranged by size:
210 166 93 89 72 69 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 166 460 720
Size: 117 49 294 260
Fragments arranged by size:
294 260 117 49
BsiWI C'GTAC_G
Cuts at: 0 15 720
Size: 15 705
Bsp1286I G_dGCh'C
Cuts at: 0 396 720
Size: 396 324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 234 720
Size: 234 486
BsrFI r'CCGG_y
Cuts at: 0 316 720
Size: 316 404
BssSI C'ACGA_G
Cuts at: 0 63 268 720
Size: 63 205 452
Fragments arranged by size:
452 205 63
BstEII G'GTnAC_C
Cuts at: 0 204 402 720
Size: 204 198 318
Fragments arranged by size:
318 204 198
Bsu36I CC'TnA_GG
Cuts at: 0 367 720
Size: 367 353
BtgI C'CryG_G
Cuts at: 0 489 720
Size: 489 231
BtsI GCAGTG_nn'
Cuts at: 0 105 720
Size: 105 615
EaeI y'GGCC_r
Cuts at: 0 152 473 720
Size: 152 321 247
Fragments arranged by size:
321 247 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 550 646 720
Size: 291 259 96 74
Fragments arranged by size:
291 259 96 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 366 720
Size: 366 354
EcoO109I rG'GnC_Cy
Cuts at: 0 372 392 393 720
Size: 372 20 1 327
Fragments arranged by size:
372 327 20 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 188 220 247 279 720
Size: 188 32 27 32 441
Fragments arranged by size:
441 188 32 32 27
HincII GTy'rAC
Cuts at: 0 276 720
Size: 276 444
KpnI G_GTAC'C
Cuts at: 0 692 720
Size: 692 28
MscI TGG'CCA
Cuts at: 0 475 720
Size: 475 245
MspA1I CmG'CkG
Cuts at: 0 180 246 323 333 351 444 465 720
Size: 180 66 77 10 18 93 21 255
Fragments arranged by size:
255 180 93 77 66 21 18 10
PspOMI G'GGCC_C
Cuts at: 0 392 720
Size: 392 328
PstI C_TGCA'G
Cuts at: 0 179 231 720
Size: 179 52 489
Fragments arranged by size:
489 179 52
PvuII CAG'CTG
Cuts at: 0 180 246 351 444 720
Size: 180 66 105 93 276
Fragments arranged by size:
276 180 105 93 66
SbfI CC_TGCA'GG
Cuts at: 0 231 720
Size: 231 489
SfcI C'TryA_G
Cuts at: 0 175 227 720
Size: 175 52 493
Fragments arranged by size:
493 175 52
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 151 720
Size: 151 569
SmaI CCC'GGG
Cuts at: 0 109 583 720
Size: 109 474 137
Fragments arranged by size:
474 137 109
StyI C'CwwG_G
Cuts at: 0 159 434 720
Size: 159 275 286
Fragments arranged by size:
286 275 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 64 502 598 720
Size: 64 438 96 122
Fragments arranged by size:
438 122 96 64
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 720
Size: 441 279
XmaI C'CCGG_G
Cuts at: 0 107 581 720
Size: 107 474 139
Fragments arranged by size:
474 139 107
Enzymes that do cut:
Acc65I ApaI AvaI BanI BanII BbvCI BglI Bme1580I
BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiWI Bsp1286I BspMI BsrFI BssSI BstEII Bsu36I
BtgI BtsI EaeI Eco57MI EcoNI EcoO109I Hin4I HincII
KpnI MscI MspA1I PspOMI PstI PvuII SbfI SfcI
SfiI SmaI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI BpuEI BsaAI BsaBI BsiEI BsiHKAI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI
BstBI BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
MfeI MluI MmeI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI ScaI SexAI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmnI ZraI