(Linear) (Six-base) MAPSORT of: KIEE0223.seq  Check: 6680  from: 1  to: 237



KIEE0223.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     115     237 
   Size:         49      66     122

  Fragments arranged by size: 

                 122      66      49
 
AhdI GACnn_n'nnGTC

Cuts at:      0      78     237 
   Size:         78     159
 
ApoI r'AATT_y

Cuts at:      0     115     205     237 
   Size:        115      90      32

  Fragments arranged by size: 

                 115      90      32
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     116     237 
   Size:        116     121
 
Bsu36I CC'TnA_GG

Cuts at:      0      20     237 
   Size:         20     217
 
EarI CTCTTCn'nnn_

Cuts at:      0      25     237 
   Size:         25     212
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     115     237 
   Size:         49      66     122

  Fragments arranged by size: 

                 122      66      49
 
EcoRI G'AATT_C

Cuts at:      0     115     205     237 
   Size:        115      90      32

  Fragments arranged by size: 

                 115      90      32
 
EcoRV GAT'ATC

Cuts at:      0     230     237 
   Size:        230       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      64      96     237 
   Size:         64      32     141

  Fragments arranged by size: 

                 141      64      32
 
ScaI AGT'ACT

Cuts at:      0       6     237 
   Size:          6     231
 
TatI w'GTAC_w

Cuts at:      0       4     237 
   Size:          4     233
 

 Enzymes that do cut:

     AcuI     AhdI     ApoI     BmrI   Bsu36I     EarI  Eco57MI    EcoRI
    EcoRV    Hin4I     ScaI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI