(Linear) (Six-base) MAPSORT of: KIEE0221.seq  Check: 86  from: 1  to: 288



KIEE0221.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

Acc65I G'GTAC_C

Cuts at:      0      81     288 
   Size:         81     207
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     288 
   Size:        217      71
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      91     288 
   Size:         91     197
 
BanI G'GyrC_C

Cuts at:      0      81     288 
   Size:         81     207
 
BbvCI CC'TCA_GC

Cuts at:      0     262     288 
   Size:        262      26
 
BmtI G_CTAG'C

Cuts at:      0      40     288 
   Size:         40     248
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     288 
   Size:         75     213
 
Bpu10I CC'TnA_GC

Cuts at:      0     262     288 
   Size:        262      26
 
BseYI C'CCAG_C

Cuts at:      0      40     288 
   Size:         40     248
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      69     288 
   Size:         69     219
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      21     288 
   Size:         21     267
 
Bsu36I CC'TnA_GG

Cuts at:      0     130     288 
   Size:        130     158
 
BtsI GCAGTG_nn'

Cuts at:      0     267     288 
   Size:        267      21
 
EaeI y'GGCC_r

Cuts at:      0      49     288 
   Size:         49     239
 
EarI CTCTTCn'nnn_

Cuts at:      0     193     288 
   Size:        193      95
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     217     288 
   Size:         75     142      71

  Fragments arranged by size: 

                 142      75      71
 
KpnI G_GTAC'C

Cuts at:      0      85     288 
   Size:         85     203
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     288 
   Size:        213      75
 
NheI G'CTAG_C

Cuts at:      0      36     288 
   Size:         36     252
 
NspI r_CATG'y

Cuts at:      0      78     288 
   Size:         78     210
 
SphI G_CATG'C

Cuts at:      0      78     288 
   Size:         78     210
 
SspI AAT'ATT

Cuts at:      0     188     288 
   Size:        188     100
 
StuI AGG'CCT

Cuts at:      0     214     288 
   Size:        214      74
 
StyI C'CwwG_G

Cuts at:      0     221     288 
   Size:        221      67
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI     BanI    BbvCI     BmtI     BpmI   Bpu10I
    BseYI    BspMI    BstXI   Bsu36I     BtsI     EaeI     EarI  Eco57MI
     KpnI     MmeI     NheI     NspI     SphI     SspI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI