(Linear) (Six-base) MAPSORT of: KIEE0218.seq  Check: 7966  from: 1  to: 534



KIEE0218.orf

 With 182 enzymes: SgfI * 

                        October 6, 2008 10:37  ..

AccI GT'mk_AC

Cuts at:      0      20     534 
   Size:         20     514
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     336     370     534 
   Size:        336      34     164

  Fragments arranged by size: 

                 336     164      34
 
BfrBI ATG'CAT

Cuts at:      0     398     534 
   Size:        398     136
 
BlpI GC'TnA_GC

Cuts at:      0     106     534 
   Size:        106     428
 
BmgBI CAC'GTC

Cuts at:      0     186     534 
   Size:        186     348
 
BsaAI yAC'GTr

Cuts at:      0      18     534 
   Size:         18     516
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     534 
   Size:        358     176
 
BsrDI GCAATG_nn'

Cuts at:      0     510     534 
   Size:        510      24
 
BtgI C'CryG_G

Cuts at:      0      92     534 
   Size:         92     442
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     216     279     534 
   Size:        216      63     255

  Fragments arranged by size: 

                 255     216      63
 
HincII GTy'rAC

Cuts at:      0     156     534 
   Size:        156     378
 
MslI CAynn'nnrTG

Cuts at:      0     328     534 
   Size:        328     206
 
NcoI C'CATG_G

Cuts at:      0      92     534 
   Size:         92     442
 
NsiI A_TGCA'T

Cuts at:      0     400     534 
   Size:        400     134
 
PfoI T'CCnGG_A

Cuts at:      0      53     534 
   Size:         53     481
 
SexAI A'CCwGG_T

Cuts at:      0     149     534 
   Size:        149     385
 
SnaBI TAC'GTA

Cuts at:      0      18     534 
   Size:         18     516
 
SspI AAT'ATT

Cuts at:      0     497     534 
   Size:        497      37
 
StyI C'CwwG_G

Cuts at:      0      92     137     534 
   Size:         92      45     397

  Fragments arranged by size: 

                 397      92      45
 
Tth111I GACn'n_nGTC

Cuts at:      0     151     187     534 
   Size:        151      36     347

  Fragments arranged by size: 

                 347     151      36
 
XmnI GAAnn'nnTTC

Cuts at:      0     496     534 
   Size:        496      38
 

 Enzymes that do cut:

     AccI     BcgI    BfrBI     BlpI    BmgBI    BsaAI     BsgI    BsrDI
     BtgI     FalI   HincII     MslI     NcoI     NsiI     PfoI    SexAI
    SnaBI     SspI     StyI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI
    BglII Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     TatI     TstI     XbaI     XcmI     XhoI     XmaI     ZraI