Restriction map for
pF1KE0206
(Linear) (Six-base) MAPSORT of: KIEE0206.seq Check: 2219 from: 1 to: 678
KIEE0206.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 524 552 678
Size: 469 55 28 126
Fragments arranged by size:
469 126 55 28
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 611 678
Size: 611 67
BanI G'GyrC_C
Cuts at: 0 78 109 678
Size: 78 31 569
Fragments arranged by size:
569 78 31
BanII G_rGCy'C
Cuts at: 0 93 678
Size: 93 585
BbsI GAAGACnn'nnnn_
Cuts at: 0 120 678
Size: 120 558
BbvCI CC'TCA_GC
Cuts at: 0 261 678
Size: 261 417
BclI T'GATC_A
Cuts at: 0 141 678
Size: 141 537
Bme1580I G_kGCm'C
Cuts at: 0 457 678
Size: 457 221
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 107 194 678
Size: 107 87 484
Fragments arranged by size:
484 107 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 397 678
Size: 214 183 281
Fragments arranged by size:
281 214 183
Bpu10I CC'TnA_GC
Cuts at: 0 7 261 678
Size: 7 254 417
Fragments arranged by size:
417 254 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 345 678
Size: 17 328 333
Fragments arranged by size:
333 328 17
BsaI GGTCTCn'nnnn_
Cuts at: 0 21 463 678
Size: 21 442 215
Fragments arranged by size:
442 215 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 678
Size: 115 30 533
Fragments arranged by size:
533 115 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 38 481 589 592 678
Size: 38 443 108 3 86
Fragments arranged by size:
443 108 86 38 3
BseYI C'CCAG_C
Cuts at: 0 66 345 678
Size: 66 279 333
Fragments arranged by size:
333 279 66
BsiHKAI G_wGCw'C
Cuts at: 0 93 678
Size: 93 585
Bsp1286I G_dGCh'C
Cuts at: 0 93 457 678
Size: 93 364 221
Fragments arranged by size:
364 221 93
BsrDI GCAATG_nn'
Cuts at: 0 207 678
Size: 207 471
BstAPI GCAn_nnn'nTGC
Cuts at: 0 230 678
Size: 230 448
BstXI CCAn_nnnn'nTGG
Cuts at: 0 66 678
Size: 66 612
BstYI r'GATC_y
Cuts at: 0 152 678
Size: 152 526
BtgI C'CryG_G
Cuts at: 0 383 678
Size: 383 295
BtsI GCAGTG_nn'
Cuts at: 0 219 678
Size: 219 459
DraI TTT'AAA
Cuts at: 0 165 678
Size: 165 513
EarI CTCTTCn'nnn_
Cuts at: 0 81 662 678
Size: 81 581 16
Fragments arranged by size:
581 81 16
EcoICRI GAG'CTC
Cuts at: 0 91 678
Size: 91 587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 397 469 524 552 678
Size: 214 183 72 55 28 126
Fragments arranged by size:
214 183 126 72 55 28
EcoO109I rG'GnC_Cy
Cuts at: 0 396 678
Size: 396 282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 562 678
Size: 562 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 62 201 233 678
Size: 30 32 139 32 445
Fragments arranged by size:
445 139 32 32 30
HindIII A'AGCT_T
Cuts at: 0 272 678
Size: 272 406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 678
Size: 146 532
MslI CAynn'nnrTG
Cuts at: 0 283 678
Size: 283 395
NcoI C'CATG_G
Cuts at: 0 383 678
Size: 383 295
PfoI T'CCnGG_A
Cuts at: 0 155 678
Size: 155 523
SacI G_AGCT'C
Cuts at: 0 93 678
Size: 93 585
SapI GCTCTTCn'nnn_
Cuts at: 0 81 678
Size: 81 597
SfcI C'TryA_G
Cuts at: 0 633 678
Size: 633 45
SmlI C'TyrA_G
Cuts at: 0 32 324 678
Size: 32 292 354
Fragments arranged by size:
354 292 32
StyI C'CwwG_G
Cuts at: 0 383 678
Size: 383 295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 298 678
Size: 298 380
XmnI GAAnn'nnTTC
Cuts at: 0 514 678
Size: 514 164
Enzymes that do cut:
AcuI AlfI BanI BanII BbsI BbvCI BclI Bme1580I
BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BsrDI BstAPI BstXI BstYI BtgI BtsI
DraI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HindIII
MmeI MslI NcoI PfoI SacI SapI SfcI SmlI
StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EaeI EagI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MscI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
ZraI