(Linear) (Six-base) MAPSORT of: KIEE0194.seq  Check: 4010  from: 1  to: 543



KIEE0194.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     543 
   Size:        265     278
 
ApoI r'AATT_y

Cuts at:      0      71     253     344     355     543 
   Size:         71     182      91      11     188

  Fragments arranged by size: 

                 188     182      91      71      11
 
BglI GCCn_nnn'nGGC

Cuts at:      0     325     543 
   Size:        325     218
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     543 
   Size:         64     479
 
Bpu10I CC'TnA_GC

Cuts at:      0     442     543 
   Size:        442     101
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     543 
   Size:        292     251
 
BsaBI GATnn'nnATC

Cuts at:      0      58     543 
   Size:         58     485
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     357     387     543 
   Size:        357      30     156

  Fragments arranged by size: 

                 357     156      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67     543 
   Size:         67     476
 
BstEII G'GTnAC_C

Cuts at:      0     100     543 
   Size:        100     443
 
BtsI GCAGTG_nn'

Cuts at:      0     184     543 
   Size:        184     359
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     265     543 
   Size:         64     201     278

  Fragments arranged by size: 

                 278     201      64
 
EcoO109I rG'GnC_Cy

Cuts at:      0     171     543 
   Size:        171     372
 
EcoRI G'AATT_C

Cuts at:      0      71     253     355     543 
   Size:         71     182     102     188

  Fragments arranged by size: 

                 188     182     102      71
 
MslI CAynn'nnrTG

Cuts at:      0     415     543 
   Size:        415     128
 
PstI C_TGCA'G

Cuts at:      0     179     209     543 
   Size:        179      30     334

  Fragments arranged by size: 

                 334     179      30
 
SexAI A'CCwGG_T

Cuts at:      0     425     543 
   Size:        425     118
 
SfcI C'TryA_G

Cuts at:      0     175     205     543 
   Size:        175      30     338

  Fragments arranged by size: 

                 338     175      30
 
SmlI C'TyrA_G

Cuts at:      0     271     543 
   Size:        271     272
 
SspI AAT'ATT

Cuts at:      0     390     543 
   Size:        390     153
 
StyI C'CwwG_G

Cuts at:      0      47     543 
   Size:         47     496
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     273     305     543 
   Size:        273      32     238

  Fragments arranged by size: 

                 273     238      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     106     543 
   Size:        106     437
 
XmnI GAAnn'nnTTC

Cuts at:      0     359     543 
   Size:        359     184
 

 Enzymes that do cut:

     AcuI     ApoI     BglI     BpmI   Bpu10I    BpuEI    BsaBI    BsaXI
    BseRI   BstEII     BtsI  Eco57MI EcoO109I    EcoRI     MslI     PstI
    SexAI     SfcI     SmlI     SspI     StyI     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BsaI
    BsaAI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI     XbaI
     XcmI     XhoI     XmaI     ZraI