(Linear) (Six-base) MAPSORT of: KIEE0191.seq  Check: 832  from: 1  to: 393



KIEE0191.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     203     393 
   Size:        124      79     190

  Fragments arranged by size: 

                 190     124      79
 
ApoI r'AATT_y

Cuts at:      0     321     393 
   Size:        321      72
 
BanI G'GyrC_C

Cuts at:      0       3     393 
   Size:          3     390
 
BbeI G_GCGC'C

Cuts at:      0       7     393 
   Size:          7     386
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     306     393 
   Size:        306      87
 
BfrBI ATG'CAT

Cuts at:      0     340     393 
   Size:        340      53
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      43     393 
   Size:         43     350
 
BsaHI Gr'CG_yC

Cuts at:      0       4     393 
   Size:          4     389
 
BtsI GCAGTG_nn'

Cuts at:      0     118     382     393 
   Size:        118     264      11

  Fragments arranged by size: 

                 264     118      11
 
DraI TTT'AAA

Cuts at:      0     201     393 
   Size:        201     192
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     203     393 
   Size:        124      79     190

  Fragments arranged by size: 

                 190     124      79
 
FspI TGC'GCA

Cuts at:      0      74     393 
   Size:         74     319
 
HaeII r_GCGC'y

Cuts at:      0       7     393 
   Size:          7     386
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     328     360     393 
   Size:        328      32      33

  Fragments arranged by size: 

                 328      33      32
 
KasI G'GCGC_C

Cuts at:      0       3     393 
   Size:          3     390
 
MspA1I CmG'CkG

Cuts at:      0      70     393 
   Size:         70     323
 
NarI GG'CG_CC

Cuts at:      0       4     393 
   Size:          4     389
 
NsiI A_TGCA'T

Cuts at:      0     342     393 
   Size:        342      51
 
ScaI AGT'ACT

Cuts at:      0     296     393 
   Size:        296      97
 
SfoI GGC'GCC

Cuts at:      0       5     393 
   Size:          5     388
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      25     275     393 
   Size:         25     250     118

  Fragments arranged by size: 

                 250     118      25
 
TatI w'GTAC_w

Cuts at:      0     294     393 
   Size:        294      99
 

 Enzymes that do cut:

     AcuI     ApoI     BanI     BbeI    BciVI    BfrBI     BsaI    BsaHI
     BtsI     DraI  Eco57MI     FspI    HaeII    Hin4I     KasI   MspA1I
     NarI     NsiI     ScaI     SfoI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbsI    BbvCI     BcgI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI