Restriction map for
pF1KE0180
(Linear) (Six-base) MAPSORT of: KIEE0180.seq Check: 7733 from: 1 to: 654
KIEE0180.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 498 654
Size: 498 156
AleI CACnn'nnGTG
Cuts at: 0 550 654
Size: 550 104
AlwNI CAG_nnn'CTG
Cuts at: 0 144 654
Size: 144 510
AvaI C'yCGr_G
Cuts at: 0 634 654
Size: 634 20
BanI G'GyrC_C
Cuts at: 0 508 654
Size: 508 146
BbsI GAAGACnn'nnnn_
Cuts at: 0 414 654
Size: 414 240
BlpI GC'TnA_GC
Cuts at: 0 55 654
Size: 55 599
Bme1580I G_kGCm'C
Cuts at: 0 633 654
Size: 633 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 654
Size: 166 488
BsaI GGTCTCn'nnnn_
Cuts at: 0 257 654
Size: 257 397
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 6 36 260 290 654
Size: 6 30 224 30 364
Fragments arranged by size:
364 224 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 528 654
Size: 528 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 180 241 295 542 654
Size: 65 115 61 54 247 112
Fragments arranged by size:
247 115 112 65 61 54
BsiHKAI G_wGCw'C
Cuts at: 0 321 654
Size: 321 333
Bsp1286I G_dGCh'C
Cuts at: 0 321 633 654
Size: 321 312 21
Fragments arranged by size:
321 312 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 84 654
Size: 84 570
BtgI C'CryG_G
Cuts at: 0 176 654
Size: 176 478
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 375 627 654
Size: 375 252 27
Fragments arranged by size:
375 252 27
BtsI GCAGTG_nn'
Cuts at: 0 338 559 654
Size: 338 221 95
Fragments arranged by size:
338 221 95
ClaI AT'CG_AT
Cuts at: 0 488 654
Size: 488 166
EaeI y'GGCC_r
Cuts at: 0 576 654
Size: 576 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 498 654
Size: 166 332 156
Fragments arranged by size:
332 166 156
EcoO109I rG'GnC_Cy
Cuts at: 0 66 654
Size: 66 588
EcoRV GAT'ATC
Cuts at: 0 492 654
Size: 492 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 6 38 654
Size: 6 32 616
Fragments arranged by size:
616 32 6
HincII GTy'rAC
Cuts at: 0 171 654
Size: 171 483
HpaI GTT'AAC
Cuts at: 0 171 654
Size: 171 483
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 654
Size: 246 408
MslI CAynn'nnrTG
Cuts at: 0 550 646 654
Size: 550 96 8
Fragments arranged by size:
550 96 8
MspA1I CmG'CkG
Cuts at: 0 348 654
Size: 348 306
PfoI T'CCnGG_A
Cuts at: 0 143 595 654
Size: 143 452 59
Fragments arranged by size:
452 143 59
PpuMI rG'GwC_Cy
Cuts at: 0 66 654
Size: 66 588
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 9 41 654
Size: 9 32 613
Fragments arranged by size:
613 32 9
PstI C_TGCA'G
Cuts at: 0 566 654
Size: 566 88
SanDI GG'GwC_CC
Cuts at: 0 66 654
Size: 66 588
SfcI C'TryA_G
Cuts at: 0 562 654
Size: 562 92
StyI C'CwwG_G
Cuts at: 0 330 654
Size: 330 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 465 631 654
Size: 465 166 23
Fragments arranged by size:
465 166 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 260 292 654
Size: 260 32 362
Fragments arranged by size:
362 260 32
Tth111I GACn'n_nGTC
Cuts at: 0 93 654
Size: 93 561
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 654
Size: 345 309
Enzymes that do cut:
AcuI AleI AlwNI AvaI BanI BbsI BlpI Bme1580I
BpmI BsaI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI
BtgI BtgZI BtsI ClaI EaeI Eco57MI EcoO109I EcoRV
Hin4I HincII HpaI MmeI MslI MspA1I PfoI PpuMI
PsrI PstI SanDI SfcI StyI TaqII TstI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoNI EcoRI FalI FseI
FspI FspAI HaeII HindIII KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI XbaI XhoI
XmaI XmnI ZraI