Restriction map for
pF1KE0178
(Linear) (Six-base) MAPSORT of: KIEE0178.seq Check: 7320 from: 1 to: 1041
KIEE0178.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 761 1041
Size: 761 280
AccI GT'mk_AC
Cuts at: 0 956 1041
Size: 956 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 650 1041
Size: 511 139 391
Fragments arranged by size:
511 391 139
AflIII A'CryG_T
Cuts at: 0 344 1041
Size: 344 697
AlwNI CAG_nnn'CTG
Cuts at: 0 49 126 808 1041
Size: 49 77 682 233
Fragments arranged by size:
682 233 77 49
ApoI r'AATT_y
Cuts at: 0 109 523 1041
Size: 109 414 518
Fragments arranged by size:
518 414 109
AseI AT'TA_AT
Cuts at: 0 208 1041
Size: 208 833
BanII G_rGCy'C
Cuts at: 0 282 452 1041
Size: 282 170 589
Fragments arranged by size:
589 282 170
BbsI GAAGACnn'nnnn_
Cuts at: 0 392 515 909 1041
Size: 392 123 394 132
Fragments arranged by size:
394 392 132 123
BciVI GTATCCnnnnn_n'
Cuts at: 0 173 1041
Size: 173 868
BclI T'GATC_A
Cuts at: 0 548 1041
Size: 548 493
BfrBI ATG'CAT
Cuts at: 0 828 1041
Size: 828 213
Bme1580I G_kGCm'C
Cuts at: 0 480 1041
Size: 480 561
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 719 1041
Size: 719 322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 1041
Size: 296 745
Bpu10I CC'TnA_GC
Cuts at: 0 260 665 1041
Size: 260 405 376
Fragments arranged by size:
405 376 260
BsaI GGTCTCn'nnnn_
Cuts at: 0 141 693 1041
Size: 141 552 348
Fragments arranged by size:
552 348 141
BsaHI Gr'CG_yC
Cuts at: 0 512 1041
Size: 512 529
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 772 802 1041
Size: 772 30 239
Fragments arranged by size:
772 239 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 711 1041
Size: 711 330
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 1041
Size: 450 591
BsiHKAI G_wGCw'C
Cuts at: 0 282 790 1041
Size: 282 508 251
Fragments arranged by size:
508 282 251
Bsp1286I G_dGCh'C
Cuts at: 0 282 452 480 790 1041
Size: 282 170 28 310 251
Fragments arranged by size:
310 282 251 170 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 761 1041
Size: 761 280
BsrBI CCG'CTC
Cuts at: 0 669 1041
Size: 669 372
BsrDI GCAATG_nn'
Cuts at: 0 329 1041
Size: 329 712
BsrFI r'CCGG_y
Cuts at: 0 70 1041
Size: 70 971
BstAPI GCAn_nnn'nTGC
Cuts at: 0 834 1041
Size: 834 207
BstXI CCAn_nnnn'nTGG
Cuts at: 0 82 1041
Size: 82 959
BstYI r'GATC_y
Cuts at: 0 3 1041
Size: 3 1038
Bsu36I CC'TnA_GG
Cuts at: 0 608 1041
Size: 608 433
BtgI C'CryG_G
Cuts at: 0 691 1041
Size: 691 350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1031 1041
Size: 1031 10
BtsI GCAGTG_nn'
Cuts at: 0 218 1041
Size: 218 823
DraI TTT'AAA
Cuts at: 0 779 1041
Size: 779 262
EaeI y'GGCC_r
Cuts at: 0 72 920 1041
Size: 72 848 121
Fragments arranged by size:
848 121 72
EarI CTCTTCn'nnn_
Cuts at: 0 572 1041
Size: 572 469
EcoICRI GAG'CTC
Cuts at: 0 280 1041
Size: 280 761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 511 650 1041
Size: 296 215 139 391
Fragments arranged by size:
391 296 215 139
EcoNI CCTnn'n_nnAGG
Cuts at: 0 571 1041
Size: 571 470
EcoRV GAT'ATC
Cuts at: 0 945 1041
Size: 945 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 807 1041
Size: 807 234
HaeII r_GCGC'y
Cuts at: 0 294 657 1041
Size: 294 363 384
Fragments arranged by size:
384 363 294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 522 554 1041
Size: 60 32 430 32 487
Fragments arranged by size:
487 430 60 32 32
HincII GTy'rAC
Cuts at: 0 15 957 1041
Size: 15 942 84
Fragments arranged by size:
942 84 15
MfeI C'AATT_G
Cuts at: 0 101 1041
Size: 101 940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 1041
Size: 551 490
MscI TGG'CCA
Cuts at: 0 922 1041
Size: 922 119
MslI CAynn'nnrTG
Cuts at: 0 80 377 1041
Size: 80 297 664
Fragments arranged by size:
664 297 80
MspA1I CmG'CkG
Cuts at: 0 123 860 1041
Size: 123 737 181
Fragments arranged by size:
737 181 123
NcoI C'CATG_G
Cuts at: 0 691 1041
Size: 691 350
NsiI A_TGCA'T
Cuts at: 0 830 1041
Size: 830 211
NspI r_CATG'y
Cuts at: 0 90 348 1041
Size: 90 258 693
Fragments arranged by size:
693 258 90
PciI A'CATG_T
Cuts at: 0 344 1041
Size: 344 697
PflMI CCAn_nnn'nTGG
Cuts at: 0 543 650 968 1005 1041
Size: 543 107 318 37 36
Fragments arranged by size:
543 318 107 37 36
PfoI T'CCnGG_A
Cuts at: 0 503 1041
Size: 503 538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 658 690 1041
Size: 658 32 351
Fragments arranged by size:
658 351 32
PvuII CAG'CTG
Cuts at: 0 123 1041
Size: 123 918
SacI G_AGCT'C
Cuts at: 0 282 1041
Size: 282 759
SalI G'TCGA_C
Cuts at: 0 955 1041
Size: 955 86
StyI C'CwwG_G
Cuts at: 0 200 517 691 1041
Size: 200 317 174 350
Fragments arranged by size:
350 317 200 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 815 979 1041
Size: 815 164 62
Fragments arranged by size:
815 164 62
TatI w'GTAC_w
Cuts at: 0 341 1041
Size: 341 700
Tth111I GACn'n_nGTC
Cuts at: 0 908 1041
Size: 908 133
XmnI GAAnn'nnTTC
Cuts at: 0 581 1041
Size: 581 460
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApoI AseI BanII
BbsI BciVI BclI BfrBI Bme1580I BplI BpmI Bpu10I
BsaI BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoRV FalI HaeII Hin4I HincII MfeI MmeI MscI
MslI MspA1I NcoI NsiI NspI PciI PflMI PfoI
PpiI PvuII SacI SalI StyI TaqII TatI Tth111I
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BglI BglII
BlpI BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsaWI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI DraIII
DrdI EagI EciI EcoO109I EcoRI FseI FspI FspAI
HindIII HpaI KasI KpnI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PasI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI XbaI XcmI
XhoI XmaI ZraI