Restriction map for
pF1KE0176
(Linear) (Six-base) MAPSORT of: KIEE0176.seq Check: 4976 from: 1 to: 2331
KIEE0176.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 0 1166 1692 2066 2305 2331
Size: 1166 526 374 239 26
Fragments arranged by size:
1166 526 374 239 26
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 1066 1103 2331
Size: 15 1051 37 1228
Fragments arranged by size:
1228 1051 37 15
AflII C'TTAA_G
Cuts at: 0 1144 2331
Size: 1144 1187
AflIII A'CryG_T
Cuts at: 0 307 1941 2331
Size: 307 1634 390
Fragments arranged by size:
1634 390 307
AleI CACnn'nnGTG
Cuts at: 0 849 2331
Size: 849 1482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2101 2331
Size: 2101 230
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 281 313 708 740 2331
Size: 281 32 395 32 1591
Fragments arranged by size:
1591 395 281 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 378 1299 2331
Size: 378 921 1032
Fragments arranged by size:
1032 921 378
ApoI r'AATT_y
Cuts at: 0 259 382 581 1549 1597 1815 2275 2331
Size: 259 123 199 968 48 218 460 56
Fragments arranged by size:
968 460 259 218 199 123 56 48
AseI AT'TA_AT
Cuts at: 0 575 2331
Size: 575 1756
AvaI C'yCGr_G
Cuts at: 0 1996 2184 2331
Size: 1996 188 147
Fragments arranged by size:
1996 188 147
AvrII C'CTAG_G
Cuts at: 0 360 2331
Size: 360 1971
BamHI G'GATC_C
Cuts at: 0 1263 2331
Size: 1263 1068
BanI G'GyrC_C
Cuts at: 0 1501 2331
Size: 1501 830
BfrBI ATG'CAT
Cuts at: 0 161 2331
Size: 161 2170
BglI GCCn_nnn'nGGC
Cuts at: 0 2018 2331
Size: 2018 313
BglII A'GATC_T
Cuts at: 0 801 809 2331
Size: 801 8 1522
Fragments arranged by size:
1522 801 8
BlpI GC'TnA_GC
Cuts at: 0 1850 2331
Size: 1850 481
BmgBI CAC'GTC
Cuts at: 0 906 2331
Size: 906 1425
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2220 2331
Size: 2220 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 499 876 1609 2131 2331
Size: 121 378 377 733 522 200
Fragments arranged by size:
733 522 378 377 200 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1245 2331
Size: 1245 1086
BsaI GGTCTCn'nnnn_
Cuts at: 0 1625 2331
Size: 1625 706
BsaAI yAC'GTr
Cuts at: 0 1942 2331
Size: 1942 389
BsaWI w'CCGG_w
Cuts at: 0 2193 2331
Size: 2193 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 113 143 708 738 2029 2059 2331
Size: 113 30 565 30 1291 30 272
Fragments arranged by size:
1291 565 272 113 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 614 617 620 623 626 629 1222 2196
Size: 614 3 3 3 3 3 593 974
Cuts at: 2196 2199 2331
Size: 3 132
Fragments arranged by size:
974 614 593 132 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 500 1988 2130 2331
Size: 500 1488 142 201
Fragments arranged by size:
1488 500 201 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1906 2262 2331
Size: 1906 356 69
Fragments arranged by size:
1906 356 69
BsiHKAI G_wGCw'C
Cuts at: 0 879 1684 2331
Size: 879 805 647
Fragments arranged by size:
879 805 647
BsmI GAATG_Cn'
Cuts at: 0 1138 1149 1763 2331
Size: 1138 11 614 568
Fragments arranged by size:
1138 614 568 11
Bsp1286I G_dGCh'C
Cuts at: 0 879 1684 2331
Size: 879 805 647
Fragments arranged by size:
879 805 647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1928 2331
Size: 1928 403
BsrDI GCAATG_nn'
Cuts at: 0 161 2331
Size: 161 2170
BstYI r'GATC_y
Cuts at: 0 801 809 1263 1487 1842 2331
Size: 801 8 454 224 355 489
Fragments arranged by size:
801 489 454 355 224 8
BstZ17I GTA'TAC
Cuts at: 0 1167 1693 2331
Size: 1167 526 638
Fragments arranged by size:
1167 638 526
Bsu36I CC'TnA_GG
Cuts at: 0 588 2331
Size: 588 1743
BtsI GCAGTG_nn'
Cuts at: 0 879 1472 2106 2331
Size: 879 593 634 225
Fragments arranged by size:
879 634 593 225
DraI TTT'AAA
Cuts at: 0 1719 2331
Size: 1719 612
EarI CTCTTCn'nnn_
Cuts at: 0 1079 1212 2331
Size: 1079 133 1119
Fragments arranged by size:
1119 1079 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 121 499 876 1066 1103 1609 2131
Size: 15 106 378 377 190 37 506 522
Cuts at: 2131 2331
Size: 200
Fragments arranged by size:
522 506 378 377 200 190 106 37
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1204 1272 2331
Size: 1204 68 1059
Fragments arranged by size:
1204 1059 68
EcoO109I rG'GnC_Cy
Cuts at: 0 35 364 454 2331
Size: 35 329 90 1877
Fragments arranged by size:
1877 329 90 35
EcoRI G'AATT_C
Cuts at: 0 581 2331
Size: 581 1750
EcoRV GAT'ATC
Cuts at: 0 749 1330 2331
Size: 749 581 1001
Fragments arranged by size:
1001 749 581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1819 2331
Size: 1819 512
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 113 145 1248 1280 1360 1392 2331
Size: 113 32 1103 32 80 32 939
Fragments arranged by size:
1103 939 113 80 32 32 32
HincII GTy'rAC
Cuts at: 0 2067 2331
Size: 2067 264
MfeI C'AATT_G
Cuts at: 0 1573 2331
Size: 1573 758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 2331
Size: 415 1916
MslI CAynn'nnrTG
Cuts at: 0 775 849 2331
Size: 775 74 1482
Fragments arranged by size:
1482 775 74
MspA1I CmG'CkG
Cuts at: 0 1416 1988 2162 2331
Size: 1416 572 174 169
Fragments arranged by size:
1416 572 174 169
NsiI A_TGCA'T
Cuts at: 0 163 2331
Size: 163 2168
NspI r_CATG'y
Cuts at: 0 311 1173 2331
Size: 311 862 1158
Fragments arranged by size:
1158 862 311
PciI A'CATG_T
Cuts at: 0 307 2331
Size: 307 2024
PflMI CCAn_nnn'nTGG
Cuts at: 0 2297 2331
Size: 2297 34
PmlI CAC'GTG
Cuts at: 0 1942 2331
Size: 1942 389
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 708 740 2331
Size: 708 32 1591
Fragments arranged by size:
1591 708 32
PpuMI rG'GwC_Cy
Cuts at: 0 35 454 2331
Size: 35 419 1877
Fragments arranged by size:
1877 419 35
PstI C_TGCA'G
Cuts at: 0 914 1437 2331
Size: 914 523 894
Fragments arranged by size:
914 894 523
PvuII CAG'CTG
Cuts at: 0 1416 2331
Size: 1416 915
SalI G'TCGA_C
Cuts at: 0 2065 2331
Size: 2065 266
SfcI C'TryA_G
Cuts at: 0 910 1193 1433 1737 2228 2331
Size: 910 283 240 304 491 103
Fragments arranged by size:
910 491 304 283 240 103
SmlI C'TyrA_G
Cuts at: 0 10 1144 1224 2331
Size: 10 1134 80 1107
Fragments arranged by size:
1134 1107 80 10
SspI AAT'ATT
Cuts at: 0 1014 2331
Size: 1014 1317
StuI AGG'CCT
Cuts at: 0 2116 2331
Size: 2116 215
StyI C'CwwG_G
Cuts at: 0 360 1807 2331
Size: 360 1447 524
Fragments arranged by size:
1447 524 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 977 1567 2005 2331
Size: 977 590 438 326
Fragments arranged by size:
977 590 438 326
TatI w'GTAC_w
Cuts at: 0 2147 2331
Size: 2147 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 744 776 2029 2061 2331
Size: 744 32 1253 32 270
Fragments arranged by size:
1253 744 270 32 32
XbaI T'CTAG_A
Cuts at: 0 1338 2331
Size: 1338 993
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 826 851 2331
Size: 826 25 1480
Fragments arranged by size:
1480 826 25
XmnI GAAnn'nnTTC
Cuts at: 0 1802 2331
Size: 1802 529
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlfI AloI AlwNI
ApoI AseI AvaI AvrII BamHI BanI BfrBI BglI
BglII BlpI BmgBI BplI BpmI BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BstYI BstZ17I Bsu36I BtsI DraI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII
MfeI MmeI MslI MspA1I NsiI NspI PciI PflMI
PmlI PpiI PpuMI PstI PvuII SalI SfcI SmlI
SspI StuI StyI TaqII TatI TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI ApaI
ApaLI AscI AsiSI BaeI BanII BbeI BbsI BbvCI
BcgI BciVI BclI Bme1580I BmrI BmtI Bpu10I BsaBI
BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BtgI
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PfoI PmeI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I
XhoI XmaI ZraI