(Linear) (Six-base) MAPSORT of: KIEE0168.seq  Check: 8344  from: 1  to: 399



KIEE0168.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     399 
   Size:        328      71
 
AflIII A'CryG_T

Cuts at:      0       9     399 
   Size:          9     390
 
AgeI A'CCGG_T

Cuts at:      0     359     399 
   Size:        359      40
 
BanI G'GyrC_C

Cuts at:      0     122     399 
   Size:        122     277
 
Bme1580I G_kGCm'C

Cuts at:      0     203     399 
   Size:        203     196
 
Bpu10I CC'TnA_GC

Cuts at:      0     330     399 
   Size:        330      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     399 
   Size:        238     161
 
BsaHI Gr'CG_yC

Cuts at:      0     214     399 
   Size:        214     185
 
BsaWI w'CCGG_w

Cuts at:      0     359     399 
   Size:        359      40
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     355     385     399 
   Size:        355      30      14

  Fragments arranged by size: 

                 355      30      14
 
BsiHKAI G_wGCw'C

Cuts at:      0     182     399 
   Size:        182     217
 
BsmI GAATG_Cn'

Cuts at:      0     394     399 
   Size:        394       5
 
Bsp1286I G_dGCh'C

Cuts at:      0     182     203     399 
   Size:        182      21     196

  Fragments arranged by size: 

                 196     182      21
 
BsrFI r'CCGG_y

Cuts at:      0     287     359     399 
   Size:        287      72      40

  Fragments arranged by size: 

                 287      72      40
 
BsrGI T'GTAC_A

Cuts at:      0     155     399 
   Size:        155     244
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     339     399 
   Size:        339      60
 
BtsI GCAGTG_nn'

Cuts at:      0     201     279     313     347     378     399 
   Size:        201      78      34      34      31      21

  Fragments arranged by size: 

                 201      78      34      34      31      21
 
DraIII CAC_nnn'GTG

Cuts at:      0     132     399 
   Size:        132     267
 
EaeI y'GGCC_r

Cuts at:      0     185     399 
   Size:        185     214
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     399 
   Size:        328      71
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     279     399 
   Size:        279     120
 
MscI TGG'CCA

Cuts at:      0     187     399 
   Size:        187     212
 
MslI CAynn'nnrTG

Cuts at:      0     128     399 
   Size:        128     271
 
NaeI GCC'GGC

Cuts at:      0     289     399 
   Size:        289     110
 
NgoMIV G'CCGG_C

Cuts at:      0     287     399 
   Size:        287     112
 
NspI r_CATG'y

Cuts at:      0      13     342     399 
   Size:         13     329      57

  Fragments arranged by size: 

                 329      57      13
 
PciI A'CATG_T

Cuts at:      0       9     399 
   Size:          9     390
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     132     399 
   Size:        132     267
 
PstI C_TGCA'G

Cuts at:      0     208     399 
   Size:        208     191
 
SfcI C'TryA_G

Cuts at:      0     204     399 
   Size:        204     195
 
SmlI C'TyrA_G

Cuts at:      0     253     399 
   Size:        253     146
 
SphI G_CATG'C

Cuts at:      0     342     399 
   Size:        342      57
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     164     399 
   Size:        164     235
 
TatI w'GTAC_w

Cuts at:      0      12     155     399 
   Size:         12     143     244

  Fragments arranged by size: 

                 244     143      12
 

 Enzymes that do cut:

     AcuI   AflIII     AgeI     BanI Bme1580I   Bpu10I    BpuEI    BsaHI
    BsaWI    BsaXI  BsiHKAI     BsmI Bsp1286I    BsrFI    BsrGI    BtgZI
     BtsI   DraIII     EaeI  Eco57MI     FalI     MscI     MslI     NaeI
   NgoMIV     NspI     PciI    PflMI     PstI     SfcI     SmlI     SphI
    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI
    BsaBI    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI
     DraI     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI