Restriction map for
pF1KE0164
(Linear) (Six-base) MAPSORT of: KIEE0164.seq Check: 8719 from: 1 to: 414
KIEE0164.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 135 414
Size: 135 279
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 414
Size: 286 128
AfeI AGC'GCT
Cuts at: 0 166 414
Size: 166 248
AlwNI CAG_nnn'CTG
Cuts at: 0 318 414
Size: 318 96
ApaLI G'TGCA_C
Cuts at: 0 148 243 414
Size: 148 95 171
Fragments arranged by size:
171 148 95
BfrBI ATG'CAT
Cuts at: 0 252 414
Size: 252 162
BlpI GC'TnA_GC
Cuts at: 0 5 414
Size: 5 409
Bme1580I G_kGCm'C
Cuts at: 0 152 247 414
Size: 152 95 167
Fragments arranged by size:
167 152 95
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 414
Size: 235 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 414
Size: 170 244
BsaI GGTCTCn'nnnn_
Cuts at: 0 94 414
Size: 94 320
BsaWI w'CCGG_w
Cuts at: 0 74 98 414
Size: 74 24 316
Fragments arranged by size:
316 74 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 66 414
Size: 66 348
BseYI C'CCAG_C
Cuts at: 0 286 414
Size: 286 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 123 414
Size: 46 77 291
Fragments arranged by size:
291 77 46
BsiHKAI G_wGCw'C
Cuts at: 0 152 247 414
Size: 152 95 167
Fragments arranged by size:
167 152 95
Bsp1286I G_dGCh'C
Cuts at: 0 152 247 414
Size: 152 95 167
Fragments arranged by size:
167 152 95
BspEI T'CCGG_A
Cuts at: 0 74 414
Size: 74 340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 135 414
Size: 135 279
BsrDI GCAATG_nn'
Cuts at: 0 252 264 414
Size: 252 12 150
Fragments arranged by size:
252 150 12
BstEII G'GTnAC_C
Cuts at: 0 201 414
Size: 201 213
BstXI CCAn_nnnn'nTGG
Cuts at: 0 213 342 414
Size: 213 129 72
Fragments arranged by size:
213 129 72
BtgI C'CryG_G
Cuts at: 0 44 414
Size: 44 370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 286 414
Size: 235 51 128
Fragments arranged by size:
235 128 51
HaeII r_GCGC'y
Cuts at: 0 110 168 414
Size: 110 58 246
Fragments arranged by size:
246 110 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 56 72 88 104 414
Size: 56 16 16 16 310
Fragments arranged by size:
310 56 16 16 16
MfeI C'AATT_G
Cuts at: 0 404 414
Size: 404 10
MslI CAynn'nnrTG
Cuts at: 0 257 414
Size: 257 157
MspA1I CmG'CkG
Cuts at: 0 113 318 414
Size: 113 205 96
Fragments arranged by size:
205 113 96
NcoI C'CATG_G
Cuts at: 0 44 414
Size: 44 370
NsiI A_TGCA'T
Cuts at: 0 254 414
Size: 254 160
NspI r_CATG'y
Cuts at: 0 357 414
Size: 357 57
PflMI CCAn_nnn'nTGG
Cuts at: 0 198 414
Size: 198 216
PfoI T'CCnGG_A
Cuts at: 0 224 414
Size: 224 190
PstI C_TGCA'G
Cuts at: 0 14 414
Size: 14 400
PvuII CAG'CTG
Cuts at: 0 113 318 414
Size: 113 205 96
Fragments arranged by size:
205 113 96
SfcI C'TryA_G
Cuts at: 0 10 414
Size: 10 404
SmlI C'TyrA_G
Cuts at: 0 185 414
Size: 185 229
StuI AGG'CCT
Cuts at: 0 133 414
Size: 133 281
StyI C'CwwG_G
Cuts at: 0 44 414
Size: 44 370
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaLI BfrBI BlpI Bme1580I
BpmI BpuEI BsaI BsaWI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspMI BsrDI BstEII BstXI BtgI Eco57MI
HaeII Hin4I MfeI MslI MspA1I NcoI NsiI NspI
PflMI PfoI PstI PvuII SfcI SmlI StuI StyI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BmgBI BmrI
BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaXI BsiEI
BsiWI BsmI BsmBI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI