Restriction map for pF1KE0159
 (Linear) (Six-base) MAPSORT of: KIEE0159.seq  Check: 8095  from: 1  to: 732



KIEE0159.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     579     732 
   Size:        579     153
 
AfeI AGC'GCT

Cuts at:      0     147     732 
   Size:        147     585
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     267     732 
   Size:        267     465
 
AlwNI CAG_nnn'CTG

Cuts at:      0     489     602     732 
   Size:        489     113     130

  Fragments arranged by size: 

                 489     130     113
 
ApaI G_GGCC'C

Cuts at:      0      96     732 
   Size:         96     636
 
ApoI r'AATT_y

Cuts at:      0     616     732 
   Size:        616     116
 
AscI GG'CGCG_CC

Cuts at:      0      28     732 
   Size:         28     704
 
AvaI C'yCGr_G

Cuts at:      0     517     732 
   Size:        517     215
 
BanII G_rGCy'C

Cuts at:      0      96     670     732 
   Size:         96     574      62

  Fragments arranged by size: 

                 574      96      62
 
BbvCI CC'TCA_GC

Cuts at:      0     206     732 
   Size:        206     526
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      70     104     732 
   Size:         70      34     628

  Fragments arranged by size: 

                 628      70      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     549     732 
   Size:        549     183
 
Bme1580I G_kGCm'C

Cuts at:      0      96     732 
   Size:         96     636
 
BmtI G_CTAG'C

Cuts at:      0     152     732 
   Size:        152     580
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     336     732 
   Size:        281      55     396

  Fragments arranged by size: 

                 396     281      55
 
Bpu10I CC'TnA_GC

Cuts at:      0     206     669     732 
   Size:        206     463      63

  Fragments arranged by size: 

                 463     206      63
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     732 
   Size:        361     371
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     583     613     732 
   Size:        583      30     119

  Fragments arranged by size: 

                 583     119      30
 
BseYI C'CCAG_C

Cuts at:      0     493     720     732 
   Size:        493     227      12

  Fragments arranged by size: 

                 493     227      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     732 
   Size:        100     632
 
BsiEI CG_ry'CG

Cuts at:      0     664     732 
   Size:        664      68
 
BsiHKAI G_wGCw'C

Cuts at:      0     542     670     732 
   Size:        542     128      62

  Fragments arranged by size: 

                 542     128      62
 
Bsp1286I G_dGCh'C

Cuts at:      0      96     542     670     732 
   Size:         96     446     128      62

  Fragments arranged by size: 

                 446     128      96      62
 
BsrDI GCAATG_nn'

Cuts at:      0     285     732 
   Size:        285     447
 
BssHII G'CGCG_C

Cuts at:      0      28     732 
   Size:         28     704
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     415     732 
   Size:        415     317
 
BstEII G'GTnAC_C

Cuts at:      0     546     690     732 
   Size:        546     144      42

  Fragments arranged by size: 

                 546     144      42
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     360     732 
   Size:        360     372
 
Bsu36I CC'TnA_GG

Cuts at:      0     584     732 
   Size:        584     148
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     516     732 
   Size:        516     216
 
EaeI y'GGCC_r

Cuts at:      0     455     732 
   Size:        455     277
 
EarI CTCTTCn'nnn_

Cuts at:      0     131     224     732 
   Size:        131      93     508

  Fragments arranged by size: 

                 508     131      93
 
EcoICRI GAG'CTC

Cuts at:      0     668     732 
   Size:        668      64
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     336     579     732 
   Size:        281      55     243     153

  Fragments arranged by size: 

                 281     243     153      55
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     682     732 
   Size:        682      50
 
EcoRI G'AATT_C

Cuts at:      0     616     732 
   Size:        616     116
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     315     732 
   Size:        315     417
 
HaeII r_GCGC'y

Cuts at:      0      62     149     732 
   Size:         62      87     583

  Fragments arranged by size: 

                 583      87      62
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     583     615     732 
   Size:        583      32     117

  Fragments arranged by size: 

                 583     117      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     732 
   Size:        192     540
 
MscI TGG'CCA

Cuts at:      0     457     732 
   Size:        457     275
 
MslI CAynn'nnrTG

Cuts at:      0     535     732 
   Size:        535     197
 
MspA1I CmG'CkG

Cuts at:      0     506     732 
   Size:        506     226
 
NdeI CA'TA_TG

Cuts at:      0     334     732 
   Size:        334     398
 
NheI G'CTAG_C

Cuts at:      0     148     732 
   Size:        148     584
 
NspI r_CATG'y

Cuts at:      0     513     732 
   Size:        513     219
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     489     732 
   Size:        489     243
 
PfoI T'CCnGG_A

Cuts at:      0     601     732 
   Size:        601     131
 
PspOMI G'GGCC_C

Cuts at:      0      92     732 
   Size:         92     640
 
PstI C_TGCA'G

Cuts at:      0      21     732 
   Size:         21     711
 
SacI G_AGCT'C

Cuts at:      0     670     732 
   Size:        670      62
 
SexAI A'CCwGG_T

Cuts at:      0     194     732 
   Size:        194     538
 
SfcI C'TryA_G

Cuts at:      0      17     732 
   Size:         17     715
 
SmaI CCC'GGG

Cuts at:      0     519     732 
   Size:        519     213
 
SmlI C'TyrA_G

Cuts at:      0     376     732 
   Size:        376     356
 
SphI G_CATG'C

Cuts at:      0     513     732 
   Size:        513     219
 
StuI AGG'CCT

Cuts at:      0     389     732 
   Size:        389     343
 
StyI C'CwwG_G

Cuts at:      0     398     458     732 
   Size:        398      60     274

  Fragments arranged by size: 

                 398     274      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     214     678     732 
   Size:        214     464      54

  Fragments arranged by size: 

                 464     214      54
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     120     490     732 
   Size:        120     370     242

  Fragments arranged by size: 

                 370     242     120
 
XmaI C'CCGG_G

Cuts at:      0     517     732 
   Size:        517     215
 

 Enzymes that do cut:

     AcuI     AfeI     AlfI    AlwNI     ApaI     ApoI     AscI     AvaI
    BanII    BbvCI     BcgI    BciVI Bme1580I     BmtI     BpmI   Bpu10I
    BpuEI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BsrDI
   BssHII   BstAPI   BstEII    BstXI   Bsu36I    BtgZI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI    EcoRI     FalI    HaeII    Hin4I     MmeI
     MscI     MslI   MspA1I     NdeI     NheI     NspI    PflMI     PfoI
   PspOMI     PstI     SacI    SexAI     SfcI     SmaI     SmlI     SphI
     StuI     StyI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BplI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI    BssSI    BstBI    BstYI  BstZ17I     BtgI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
 EcoO109I    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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