(Linear) (Six-base) MAPSORT of: KIEE0157.seq  Check: 1168  from: 1  to: 387



KIEE0157.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     387 
   Size:         71     316
 
AlwNI CAG_nnn'CTG

Cuts at:      0     167     387 
   Size:        167     220
 
AvaI C'yCGr_G

Cuts at:      0      62     387 
   Size:         62     325
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     314     387 
   Size:        314      73
 
Bpu10I CC'TnA_GC

Cuts at:      0     128     387 
   Size:        128     259
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     233     263     387 
   Size:        233      30     124

  Fragments arranged by size: 

                 233     124      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     387 
   Size:         71     316
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     239     271     301     333     387 
   Size:        239      32      30      32      54

  Fragments arranged by size: 

                 239      54      32      32      30
 
HindIII A'AGCT_T

Cuts at:      0     375     387 
   Size:        375      12
 
MslI CAynn'nnrTG

Cuts at:      0     137     387 
   Size:        137     250
 
NspI r_CATG'y

Cuts at:      0     136     387 
   Size:        136     251
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     316     348     387 
   Size:        316      32      39

  Fragments arranged by size: 

                 316      39      32
 
PstI C_TGCA'G

Cuts at:      0      56     387 
   Size:         56     331
 
SfcI C'TryA_G

Cuts at:      0      52     387 
   Size:         52     335
 
SmaI CCC'GGG

Cuts at:      0      64     387 
   Size:         64     323
 
SspI AAT'ATT

Cuts at:      0     384     387 
   Size:        384       3
 
XbaI T'CTAG_A

Cuts at:      0     189     387 
   Size:        189     198
 
XmaI C'CCGG_G

Cuts at:      0      62     387 
   Size:         62     325
 
XmnI GAAnn'nnTTC

Cuts at:      0     230     387 
   Size:        230     157
 

 Enzymes that do cut:

     AcuI    AlwNI     AvaI     BbsI   Bpu10I    BsaXI  Eco57MI    Hin4I
  HindIII     MslI     NspI     PpiI     PstI     SfcI     SmaI     SspI
     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI
     ZraI