(Linear) (Six-base) MAPSORT of: KIEE0149.seq  Check: 795  from: 1  to: 468



KIEE0149.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     116     148     468 
   Size:        116      32     320

  Fragments arranged by size: 

                 320     116      32
 
AvaI C'yCGr_G

Cuts at:      0      21     468 
   Size:         21     447
 
BanI G'GyrC_C

Cuts at:      0     189     216     468 
   Size:        189      27     252

  Fragments arranged by size: 

                 252     189      27
 
BanII G_rGCy'C

Cuts at:      0      82     251     468 
   Size:         82     169     217

  Fragments arranged by size: 

                 217     169      82
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     106     468 
   Size:        106     362
 
BbvCI CC'TCA_GC

Cuts at:      0     254     468 
   Size:        254     214
 
BglI GCCn_nnn'nGGC

Cuts at:      0      46     187     468 
   Size:         46     141     281

  Fragments arranged by size: 

                 281     141      46
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      77     468 
   Size:         77     391
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     468 
   Size:        349     119
 
Bpu10I CC'TnA_GC

Cuts at:      0     254     468 
   Size:        254     214
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      57     289     468 
   Size:         57     232     179

  Fragments arranged by size: 

                 232     179      57
 
BseYI C'CCAG_C

Cuts at:      0     226     238     296     468 
   Size:        226      12      58     172

  Fragments arranged by size: 

                 226     172      58      12
 
BsiEI CG_ry'CG

Cuts at:      0     182     468 
   Size:        182     286
 
BsiHKAI G_wGCw'C

Cuts at:      0     251     468 
   Size:        251     217
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     138     468 
   Size:        138     330
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     251     468 
   Size:         82     169     217

  Fragments arranged by size: 

                 217     169      82
 
BsrBI CCG'CTC

Cuts at:      0     206     468 
   Size:        206     262
 
BssSI C'ACGA_G

Cuts at:      0     259     468 
   Size:        259     209
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     264     468 
   Size:        264     204
 
Bsu36I CC'TnA_GG

Cuts at:      0     194     468 
   Size:        194     274
 
BtgI C'CryG_G

Cuts at:      0      41     468 
   Size:         41     427
 
EaeI y'GGCC_r

Cuts at:      0      32     113     179     468 
   Size:         32      81      66     289

  Fragments arranged by size: 

                 289      81      66      32
 
EagI C'GGCC_G

Cuts at:      0     179     468 
   Size:        179     289
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     214     468 
   Size:        214     254
 
EcoICRI GAG'CTC

Cuts at:      0     249     468 
   Size:        249     219
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     468 
   Size:        349     119
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     313     468 
   Size:        313     155
 
EcoO109I rG'GnC_Cy

Cuts at:      0      25     293     468 
   Size:         25     268     175

  Fragments arranged by size: 

                 268     175      25
 
MscI TGG'CCA

Cuts at:      0     115     468 
   Size:        115     353
 
MspA1I CmG'CkG

Cuts at:      0     214     468 
   Size:        214     254
 
PpuMI rG'GwC_Cy

Cuts at:      0      25     293     468 
   Size:         25     268     175

  Fragments arranged by size: 

                 268     175      25
 
SacI G_AGCT'C

Cuts at:      0     251     468 
   Size:        251     217
 
SanDI GG'GwC_CC

Cuts at:      0     293     468 
   Size:        293     175
 
StyI C'CwwG_G

Cuts at:      0      35     107     468 
   Size:         35      72     361

  Fragments arranged by size: 

                 361      72      35
 

 Enzymes that do cut:

     AloI     AvaI     BanI    BanII     BbsI    BbvCI     BglI     BmrI
     BpmI   Bpu10I    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BsrBI    BssSI   BstAPI   Bsu36I     BtgI     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     MscI   MspA1I    PpuMI     SacI
    SanDI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EarI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI