Restriction map for
pF1KE0148
(Linear) (Six-base) MAPSORT of: KIEE0148.seq Check: 4530 from: 1 to: 678
KIEE0148.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 632 678
Size: 632 46
AflIII A'CryG_T
Cuts at: 0 584 678
Size: 584 94
AhdI GACnn_n'nnGTC
Cuts at: 0 42 678
Size: 42 636
AleI CACnn'nnGTG
Cuts at: 0 313 678
Size: 313 365
ApaI G_GGCC'C
Cuts at: 0 8 649 678
Size: 8 641 29
Fragments arranged by size:
641 29 8
AvaI C'yCGr_G
Cuts at: 0 353 678
Size: 353 325
BanII G_rGCy'C
Cuts at: 0 8 29 649 678
Size: 8 21 620 29
Fragments arranged by size:
620 29 21 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 395 678
Size: 395 283
Bme1580I G_kGCm'C
Cuts at: 0 8 649 678
Size: 8 641 29
Fragments arranged by size:
641 29 8
BmgBI CAC'GTC
Cuts at: 0 39 678
Size: 39 639
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 214 361 641 678
Size: 149 65 147 280 37
Fragments arranged by size:
280 149 147 65 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 678
Size: 487 191
BsaI GGTCTCn'nnnn_
Cuts at: 0 502 678
Size: 502 176
BsaBI GATnn'nnATC
Cuts at: 0 238 678
Size: 238 440
BsaWI w'CCGG_w
Cuts at: 0 602 678
Size: 602 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 212 242 334 364 678
Size: 212 30 92 30 314
Fragments arranged by size:
314 212 92 30 30
BseYI C'CCAG_C
Cuts at: 0 60 121 627 678
Size: 60 61 506 51
Fragments arranged by size:
506 61 60 51
BsiHKAI G_wGCw'C
Cuts at: 0 29 678
Size: 29 649
BsmI GAATG_Cn'
Cuts at: 0 672 678
Size: 672 6
Bsp1286I G_dGCh'C
Cuts at: 0 8 29 649 678
Size: 8 21 620 29
Fragments arranged by size:
620 29 21 8
BspHI T'CATG_A
Cuts at: 0 140 678
Size: 140 538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 632 678
Size: 116 516 46
Fragments arranged by size:
516 116 46
BsrFI r'CCGG_y
Cuts at: 0 80 678
Size: 80 598
BstXI CCAn_nnnn'nTGG
Cuts at: 0 558 571 678
Size: 558 13 107
Fragments arranged by size:
558 107 13
BstYI r'GATC_y
Cuts at: 0 71 678
Size: 71 607
BtgI C'CryG_G
Cuts at: 0 20 557 678
Size: 20 537 121
Fragments arranged by size:
537 121 20
EaeI y'GGCC_r
Cuts at: 0 573 678
Size: 573 105
EarI CTCTTCn'nnn_
Cuts at: 0 34 678
Size: 34 644
EcoICRI GAG'CTC
Cuts at: 0 27 678
Size: 27 651
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 214 361 641 678
Size: 149 65 147 280 37
Fragments arranged by size:
280 149 147 65 37
EcoO109I rG'GnC_Cy
Cuts at: 0 4 5 646 678
Size: 4 1 641 32
Fragments arranged by size:
641 32 4 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 276 678
Size: 276 402
HaeII r_GCGC'y
Cuts at: 0 484 678
Size: 484 194
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 210 212 242 244 247 279
Size: 28 32 150 2 30 2 3 32
Cuts at: 279 334 366 678
Size: 55 32 312
Fragments arranged by size:
312 150 55 32 32 32 30 28
3 2 2
MscI TGG'CCA
Cuts at: 0 575 678
Size: 575 103
MslI CAynn'nnrTG
Cuts at: 0 313 556 569 678
Size: 313 243 13 109
Fragments arranged by size:
313 243 109 13
NspI r_CATG'y
Cuts at: 0 588 678
Size: 588 90
PasI CC'CwG_GG
Cuts at: 0 456 678
Size: 456 222
PciI A'CATG_T
Cuts at: 0 584 678
Size: 584 94
PspOMI G'GGCC_C
Cuts at: 0 4 645 678
Size: 4 641 33
Fragments arranged by size:
641 33 4
PstI C_TGCA'G
Cuts at: 0 113 426 447 529 678
Size: 113 313 21 82 149
Fragments arranged by size:
313 149 113 82 21
SacI G_AGCT'C
Cuts at: 0 29 678
Size: 29 649
SapI GCTCTTCn'nnn_
Cuts at: 0 34 678
Size: 34 644
SfcI C'TryA_G
Cuts at: 0 109 422 443 525 678
Size: 109 313 21 82 153
Fragments arranged by size:
313 153 109 82 21
SmaI CCC'GGG
Cuts at: 0 355 678
Size: 355 323
SmlI C'TyrA_G
Cuts at: 0 502 678
Size: 502 176
StyI C'CwwG_G
Cuts at: 0 251 678
Size: 251 427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 589 678
Size: 589 89
XmaI C'CCGG_G
Cuts at: 0 353 678
Size: 353 325
Enzymes that do cut:
AarI AflIII AhdI AleI ApaI AvaI BanII BbsI
Bme1580I BmgBI BpmI BpuEI BsaI BsaBI BsaWI BsaXI
BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI BstXI
BstYI BtgI EaeI EarI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I MscI MslI NspI PasI PciI PspOMI
PstI SacI SapI SfcI SmaI SmlI StyI XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmrI BmtI BplI
Bpu10I BsaAI BsaHI BseRI BsgI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmnI ZraI