(Linear) (Six-base) MAPSORT of: KIEE0144.seq  Check: 3249  from: 1  to: 501



KIEE0144.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     446     501 
   Size:        446      55
 
AvaI C'yCGr_G

Cuts at:      0      74     501 
   Size:         74     427
 
BanII G_rGCy'C

Cuts at:      0      61     198     386     501 
   Size:         61     137     188     115

  Fragments arranged by size: 

                 188     137     115      61
 
BclI T'GATC_A

Cuts at:      0     120     501 
   Size:        120     381
 
BglI GCCn_nnn'nGGC

Cuts at:      0     448     501 
   Size:        448      53
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     285     501 
   Size:        182     103     216

  Fragments arranged by size: 

                 216     182     103
 
BseYI C'CCAG_C

Cuts at:      0      10     501 
   Size:         10     491
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     501 
   Size:        391     110
 
BsiHKAI G_wGCw'C

Cuts at:      0     386     501 
   Size:        386     115
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     198     386     501 
   Size:         61     137     188     115

  Fragments arranged by size: 

                 188     137     115      61
 
BtsI GCAGTG_nn'

Cuts at:      0     408     501 
   Size:        408      93
 
DraI TTT'AAA

Cuts at:      0     270     501 
   Size:        270     231
 
EaeI y'GGCC_r

Cuts at:      0      18     501 
   Size:         18     483
 
EcoICRI GAG'CTC

Cuts at:      0     384     501 
   Size:        384     117
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     285     446     501 
   Size:        182     103     161      55

  Fragments arranged by size: 

                 182     161     103      55
 
EcoO109I rG'GnC_Cy

Cuts at:      0     149     501 
   Size:        149     352
 
EcoRV GAT'ATC

Cuts at:      0     372     501 
   Size:        372     129
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     148     180     501 
   Size:        148      32     321

  Fragments arranged by size: 

                 321     148      32
 
MfeI C'AATT_G

Cuts at:      0     293     501 
   Size:        293     208
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     156     501 
   Size:        156     345
 
NspI r_CATG'y

Cuts at:      0     206     501 
   Size:        206     295
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     205     320     501 
   Size:        205     115     181

  Fragments arranged by size: 

                 205     181     115
 
PpuMI rG'GwC_Cy

Cuts at:      0     149     501 
   Size:        149     352
 
SacI G_AGCT'C

Cuts at:      0     386     501 
   Size:        386     115
 
SanDI GG'GwC_CC

Cuts at:      0     149     501 
   Size:        149     352
 
SmaI CCC'GGG

Cuts at:      0      76     501 
   Size:         76     425
 
StyI C'CwwG_G

Cuts at:      0     377     501 
   Size:        377     124
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     312     501 
   Size:        312     189
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     323     355     501 
   Size:        323      32     146

  Fragments arranged by size: 

                 323     146      32
 
XmaI C'CCGG_G

Cuts at:      0      74     501 
   Size:         74     427
 

 Enzymes that do cut:

     AcuI     AvaI    BanII     BclI     BglI     BpmI    BseYI     BsgI
  BsiHKAI Bsp1286I     BtsI     DraI     EaeI  EcoICRI  Eco57MI EcoO109I
    EcoRV    Hin4I     MfeI     MmeI     NspI    PflMI    PpuMI     SacI
    SanDI     SmaI     StyI    TaqII     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EagI     EarI     EciI    EcoNI    EcoRI     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI