Restriction map for
pF1KE0138
(Linear) (Six-base) MAPSORT of: KIEE0138.seq Check: 8343 from: 1 to: 810
KIEE0138.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 191 810
Size: 191 619
AlwNI CAG_nnn'CTG
Cuts at: 0 15 504 524 810
Size: 15 489 20 286
Fragments arranged by size:
489 286 20 15
ApaI G_GGCC'C
Cuts at: 0 785 810
Size: 785 25
AvaI C'yCGr_G
Cuts at: 0 157 776 810
Size: 157 619 34
Fragments arranged by size:
619 157 34
BanI G'GyrC_C
Cuts at: 0 162 276 810
Size: 162 114 534
Fragments arranged by size:
534 162 114
BanII G_rGCy'C
Cuts at: 0 785 810
Size: 785 25
BbeI G_GCGC'C
Cuts at: 0 166 810
Size: 166 644
BbsI GAAGACnn'nnnn_
Cuts at: 0 545 810
Size: 545 265
BglI GCCn_nnn'nGGC
Cuts at: 0 115 212 810
Size: 115 97 598
Fragments arranged by size:
598 115 97
Bme1580I G_kGCm'C
Cuts at: 0 281 785 810
Size: 281 504 25
Fragments arranged by size:
504 281 25
BmgBI CAC'GTC
Cuts at: 0 597 810
Size: 597 213
BmrI ACTGGGnnnn_n'
Cuts at: 0 534 810
Size: 534 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 292 501 649 733 810
Size: 273 19 209 148 84 77
Fragments arranged by size:
273 209 148 84 77 19
Bpu10I CC'TnA_GC
Cuts at: 0 91 810
Size: 91 719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 810
Size: 425 385
BsaI GGTCTCn'nnnn_
Cuts at: 0 115 810
Size: 115 695
BsaHI Gr'CG_yC
Cuts at: 0 163 810
Size: 163 647
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 549 598 601 810
Size: 549 49 3 209
Fragments arranged by size:
549 209 49 3
BseYI C'CCAG_C
Cuts at: 0 45 810
Size: 45 765
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 586 810
Size: 136 450 224
Fragments arranged by size:
450 224 136
BsiEI CG_ry'CG
Cuts at: 0 230 810
Size: 230 580
BsiHKAI G_wGCw'C
Cuts at: 0 559 810
Size: 559 251
Bsp1286I G_dGCh'C
Cuts at: 0 281 559 785 810
Size: 281 278 226 25
Fragments arranged by size:
281 278 226 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 191 809 810
Size: 191 618 1
Fragments arranged by size:
618 191 1
BsrFI r'CCGG_y
Cuts at: 0 202 283 304 641 810
Size: 202 81 21 337 169
Fragments arranged by size:
337 202 169 81 21
BssHII G'CGCG_C
Cuts at: 0 55 57 755 810
Size: 55 2 698 55
Fragments arranged by size:
698 55 55 2
BssSI C'ACGA_G
Cuts at: 0 321 810
Size: 321 489
BstXI CCAn_nnnn'nTGG
Cuts at: 0 144 810
Size: 144 666
Bsu36I CC'TnA_GG
Cuts at: 0 370 810
Size: 370 440
BtgI C'CryG_G
Cuts at: 0 338 810
Size: 338 472
DraIII CAC_nnn'GTG
Cuts at: 0 339 810
Size: 339 471
EaeI y'GGCC_r
Cuts at: 0 116 204 227 620 810
Size: 116 88 23 393 190
Fragments arranged by size:
393 190 116 88 23
EagI C'GGCC_G
Cuts at: 0 227 810
Size: 227 583
EarI CTCTTCn'nnn_
Cuts at: 0 223 810
Size: 223 587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 292 501 649 733 810
Size: 273 19 209 148 84 77
Fragments arranged by size:
273 209 148 84 77 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 94 810
Size: 94 716
EcoO109I rG'GnC_Cy
Cuts at: 0 40 86 213 677 781 810
Size: 40 46 127 464 104 29
Fragments arranged by size:
464 127 104 46 40 29
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 696 810
Size: 696 114
HaeII r_GCGC'y
Cuts at: 0 52 151 166 810
Size: 52 99 15 644
Fragments arranged by size:
644 99 52 15
KasI G'GCGC_C
Cuts at: 0 162 810
Size: 162 648
MscI TGG'CCA
Cuts at: 0 622 810
Size: 622 188
MspA1I CmG'CkG
Cuts at: 0 12 69 504 810
Size: 12 57 435 306
Fragments arranged by size:
435 306 57 12
NaeI GCC'GGC
Cuts at: 0 204 285 810
Size: 204 81 525
Fragments arranged by size:
525 204 81
NarI GG'CG_CC
Cuts at: 0 163 810
Size: 163 647
NgoMIV G'CCGG_C
Cuts at: 0 202 283 810
Size: 202 81 527
Fragments arranged by size:
527 202 81
NspI r_CATG'y
Cuts at: 0 270 810
Size: 270 540
PflMI CCAn_nnn'nTGG
Cuts at: 0 339 524 810
Size: 339 185 286
Fragments arranged by size:
339 286 185
PfoI T'CCnGG_A
Cuts at: 0 344 533 650 810
Size: 344 189 117 160
Fragments arranged by size:
344 189 160 117
PpuMI rG'GwC_Cy
Cuts at: 0 40 677 810
Size: 40 637 133
Fragments arranged by size:
637 133 40
PsiI TTA'TAA
Cuts at: 0 389 810
Size: 389 421
PspOMI G'GGCC_C
Cuts at: 0 781 810
Size: 781 29
PstI C_TGCA'G
Cuts at: 0 611 810
Size: 611 199
PvuII CAG'CTG
Cuts at: 0 12 69 504 810
Size: 12 57 435 306
Fragments arranged by size:
435 306 57 12
SanDI GG'GwC_CC
Cuts at: 0 40 810
Size: 40 770
SfcI C'TryA_G
Cuts at: 0 607 810
Size: 607 203
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 212 810
Size: 212 598
SfoI GGC'GCC
Cuts at: 0 164 810
Size: 164 646
SmaI CCC'GGG
Cuts at: 0 778 810
Size: 778 32
SmlI C'TyrA_G
Cuts at: 0 440 810
Size: 440 370
SphI G_CATG'C
Cuts at: 0 270 810
Size: 270 540
StuI AGG'CCT
Cuts at: 0 574 810
Size: 574 236
Tth111I GACn'n_nGTC
Cuts at: 0 595 810
Size: 595 215
XmaI C'CCGG_G
Cuts at: 0 776 810
Size: 776 34
Enzymes that do cut:
AarI AlwNI ApaI AvaI BanI BanII BbeI BbsI
BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrFI BssHII BssSI BstXI Bsu36I BtgI DraIII EaeI
EagI EarI Eco57MI EcoNI EcoO109I FalI HaeII KasI
MscI MspA1I NaeI NarI NgoMIV NspI PflMI PfoI
PpuMI PsiI PspOMI PstI PvuII SanDI SfcI SfiI
SfoI SmaI SmlI SphI StuI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmtI BplI BsaAI BsaBI
BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BstAPI BstBI BstEII BstYI BstZ17I BtgZI
BtsI ClaI CspCI DraI DrdI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MslI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI
SpeI SrfI SspI StyI SwaI TaqII TatI TstI
XbaI XcmI XhoI XmnI ZraI