(Linear) (Six-base) MAPSORT of: KIEE0128.seq  Check: 889  from: 1  to: 303



KIEE0128.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     303 
   Size:        100     203
 
AleI CACnn'nnGTG

Cuts at:      0     279     303 
   Size:        279      24
 
BanI G'GyrC_C

Cuts at:      0     163     303 
   Size:        163     140
 
BbeI G_GCGC'C

Cuts at:      0     167     303 
   Size:        167     136
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      88     303 
   Size:         88     215
 
BclI T'GATC_A

Cuts at:      0     110     303 
   Size:        110     193
 
BsaHI Gr'CG_yC

Cuts at:      0     164     303 
   Size:        164     139
 
BtsI GCAGTG_nn'

Cuts at:      0      31     221     303 
   Size:         31     190      82

  Fragments arranged by size: 

                 190      82      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     303 
   Size:        100     203
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     184     303 
   Size:        184     119
 
HaeII r_GCGC'y

Cuts at:      0       9      25     167     303 
   Size:          9      16     142     136

  Fragments arranged by size: 

                 142     136      16       9
 
KasI G'GCGC_C

Cuts at:      0     163     303 
   Size:        163     140
 
MslI CAynn'nnrTG

Cuts at:      0     279     303 
   Size:        279      24
 
NarI GG'CG_CC

Cuts at:      0     164     303 
   Size:        164     139
 
PstI C_TGCA'G

Cuts at:      0      38     303 
   Size:         38     265
 
SfcI C'TryA_G

Cuts at:      0      34     303 
   Size:         34     269
 
SfoI GGC'GCC

Cuts at:      0     165     303 
   Size:        165     138
 
StyI C'CwwG_G

Cuts at:      0     168     303 
   Size:        168     135
 

 Enzymes that do cut:

     AcuI     AleI     BanI     BbeI     BbsI     BclI    BsaHI     BtsI
  Eco57MI     FalI    HaeII     KasI     MslI     NarI     PstI     SfcI
     SfoI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI