(Linear) (Six-base) MAPSORT of: KIEE0113.seq  Check: 5507  from: 1  to: 264



KIEE0113.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AleI CACnn'nnGTG

Cuts at:      0      31     264 
   Size:         31     233
 
BglI GCCn_nnn'nGGC

Cuts at:      0      67      76     264 
   Size:         67       9     188

  Fragments arranged by size: 

                 188      67       9
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     157     264 
   Size:        157     107
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     154     264 
   Size:        154     110
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     264 
   Size:        208      56
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     113     264 
   Size:        113     151
 
BsrDI GCAATG_nn'

Cuts at:      0      56     264 
   Size:         56     208
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      33     264 
   Size:         33     231
 
BtgI C'CryG_G

Cuts at:      0      26     258     264 
   Size:         26     232       6

  Fragments arranged by size: 

                 232      26       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      48     264 
   Size:         48     216
 
EaeI y'GGCC_r

Cuts at:      0     197     264 
   Size:        197      67
 
EcoO109I rG'GnC_Cy

Cuts at:      0      77     264 
   Size:         77     187
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     192     264 
   Size:        192      72
 
MslI CAynn'nnrTG

Cuts at:      0      31     264 
   Size:         31     233
 
PasI CC'CwG_GG

Cuts at:      0      72     264 
   Size:         72     192
 
PstI C_TGCA'G

Cuts at:      0     116     264 
   Size:        116     148
 
SfcI C'TryA_G

Cuts at:      0     112     264 
   Size:        112     152
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      76     264 
   Size:         76     188
 
StyI C'CwwG_G

Cuts at:      0     167     200     264 
   Size:        167      33      64

  Fragments arranged by size: 

                 167      64      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     197     229     264 
   Size:        197      32      35

  Fragments arranged by size: 

                 197      35      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      30      69     264 
   Size:         30      39     195

  Fragments arranged by size: 

                 195      39      30
 

 Enzymes that do cut:

     AleI     BglI     BsaI    BseRI     BsgI    BspMI    BsrDI    BstXI
     BtgI    BtgZI     EaeI EcoO109I     FalI     MslI     PasI     PstI
     SfcI     SfiI     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI