Restriction map for
pF1KE0108
(Linear) (Six-base) MAPSORT of: KIEE0108.seq Check: 1898 from: 1 to: 954
KIEE0108.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AatII G_ACGT'C
Cuts at: 0 291 800 954
Size: 291 509 154
Fragments arranged by size:
509 291 154
Acc65I G'GTAC_C
Cuts at: 0 13 954
Size: 13 941
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 913 954
Size: 610 303 41
Fragments arranged by size:
610 303 41
AgeI A'CCGG_T
Cuts at: 0 5 954
Size: 5 949
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 200 738 954
Size: 200 538 216
Fragments arranged by size:
538 216 200
ApoI r'AATT_y
Cuts at: 0 144 228 335 954
Size: 144 84 107 619
Fragments arranged by size:
619 144 107 84
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 746 779 954
Size: 746 33 175
Fragments arranged by size:
746 175 33
BamHI G'GATC_C
Cuts at: 0 887 954
Size: 887 67
BanI G'GyrC_C
Cuts at: 0 13 184 954
Size: 13 171 770
Fragments arranged by size:
770 171 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 171 954
Size: 171 783
BlpI GC'TnA_GC
Cuts at: 0 487 954
Size: 487 467
Bme1580I G_kGCm'C
Cuts at: 0 189 954
Size: 189 765
BmrI ACTGGGnnnn_n'
Cuts at: 0 597 721 954
Size: 597 124 233
Fragments arranged by size:
597 233 124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 928 954
Size: 928 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 731 954
Size: 157 574 223
Fragments arranged by size:
574 223 157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 954
Size: 454 500
BsaI GGTCTCn'nnnn_
Cuts at: 0 468 954
Size: 468 486
BsaHI Gr'CG_yC
Cuts at: 0 288 797 954
Size: 288 509 157
Fragments arranged by size:
509 288 157
BsaWI w'CCGG_w
Cuts at: 0 5 954
Size: 5 949
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 954
Size: 160 794
BseYI C'CCAG_C
Cuts at: 0 188 954
Size: 188 766
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 954
Size: 616 338
BsmBI CGTCTCn'nnnn_
Cuts at: 0 279 954
Size: 279 675
Bsp1286I G_dGCh'C
Cuts at: 0 189 954
Size: 189 765
BsrBI CCG'CTC
Cuts at: 0 879 954
Size: 879 75
BsrDI GCAATG_nn'
Cuts at: 0 75 727 954
Size: 75 652 227
Fragments arranged by size:
652 227 75
BsrFI r'CCGG_y
Cuts at: 0 5 954
Size: 5 949
BstBI TT'CG_AA
Cuts at: 0 339 954
Size: 339 615
BstXI CCAn_nnnn'nTGG
Cuts at: 0 181 954
Size: 181 773
BstYI r'GATC_y
Cuts at: 0 887 954
Size: 887 67
BtgI C'CryG_G
Cuts at: 0 32 947 954
Size: 32 915 7
Fragments arranged by size:
915 32 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 225 954
Size: 225 729
BtsI GCAGTG_nn'
Cuts at: 0 722 954
Size: 722 232
EarI CTCTTCn'nnn_
Cuts at: 0 261 576 954
Size: 261 315 378
Fragments arranged by size:
378 315 261
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 675 954
Size: 675 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 610 731 913 954
Size: 157 453 121 182 41
Fragments arranged by size:
453 182 157 121 41
EcoRI G'AATT_C
Cuts at: 0 228 335 954
Size: 228 107 619
Fragments arranged by size:
619 228 107
EcoRV GAT'ATC
Cuts at: 0 108 954
Size: 108 846
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 203 235 636 668 954
Size: 203 32 401 32 286
Fragments arranged by size:
401 286 203 32 32
KpnI G_GTAC'C
Cuts at: 0 17 954
Size: 17 937
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 828 954
Size: 246 582 126
Fragments arranged by size:
582 246 126
PfoI T'CCnGG_A
Cuts at: 0 708 954
Size: 708 246
PstI C_TGCA'G
Cuts at: 0 830 954
Size: 830 124
SfcI C'TryA_G
Cuts at: 0 826 954
Size: 826 128
SmlI C'TyrA_G
Cuts at: 0 469 954
Size: 469 485
SpeI A'CTAG_T
Cuts at: 0 247 954
Size: 247 707
SspI AAT'ATT
Cuts at: 0 397 954
Size: 397 557
StuI AGG'CCT
Cuts at: 0 587 954
Size: 587 367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 229 954
Size: 229 725
ZraI GAC'GTC
Cuts at: 0 289 798 954
Size: 289 509 156
Fragments arranged by size:
509 289 156
Enzymes that do cut:
AatII Acc65I AcuI AgeI AlfI ApoI BaeI BamHI
BanI BbsI BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaHI BsaWI BseRI BseYI BsgI BsmBI Bsp1286I
BsrBI BsrDI BsrFI BstBI BstXI BstYI BtgI BtgZI
BtsI EarI EciI Eco57MI EcoRI EcoRV Hin4I KpnI
MmeI PfoI PstI SfcI SmlI SpeI SspI StuI
XcmI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AhdI AleI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmtI Bpu10I BsaAI BsaBI BsaXI
BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrGI
BssHII BssSI BstAPI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoO109I
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI