Restriction map for
pF1KE0107
(Linear) (Six-base) MAPSORT of: KIEE0107.seq Check: 5271 from: 1 to: 849
KIEE0107.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AatII G_ACGT'C
Cuts at: 0 383 849
Size: 383 466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 539 849
Size: 64 475 310
Fragments arranged by size:
475 310 64
AflIII A'CryG_T
Cuts at: 0 316 574 849
Size: 316 258 275
Fragments arranged by size:
316 275 258
AleI CACnn'nnGTG
Cuts at: 0 424 849
Size: 424 425
AlwNI CAG_nnn'CTG
Cuts at: 0 351 412 842 849
Size: 351 61 430 7
Fragments arranged by size:
430 351 61 7
AvaI C'yCGr_G
Cuts at: 0 737 849
Size: 737 112
BanII G_rGCy'C
Cuts at: 0 241 849
Size: 241 608
BbsI GAAGACnn'nnnn_
Cuts at: 0 166 849
Size: 166 683
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 784 818 849
Size: 784 34 31
Fragments arranged by size:
784 34 31
BciVI GTATCCnnnnn_n'
Cuts at: 0 685 849
Size: 685 164
BglI GCCn_nnn'nGGC
Cuts at: 0 68 849
Size: 68 781
Bme1580I G_kGCm'C
Cuts at: 0 324 849
Size: 324 525
BmgBI CAC'GTC
Cuts at: 0 333 849
Size: 333 516
BmrI ACTGGGnnnn_n'
Cuts at: 0 530 849
Size: 530 319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 849
Size: 97 752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 849
Size: 49 800
BsaI GGTCTCn'nnnn_
Cuts at: 0 747 849
Size: 747 102
BsaAI yAC'GTr
Cuts at: 0 575 849
Size: 575 274
BsaHI Gr'CG_yC
Cuts at: 0 37 380 849
Size: 37 343 469
Fragments arranged by size:
469 343 37
BsaWI w'CCGG_w
Cuts at: 0 524 849
Size: 524 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 454 484 849
Size: 454 30 365
Fragments arranged by size:
454 365 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 97 240 849
Size: 94 3 143 609
Fragments arranged by size:
609 143 94 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 82 97 505 849
Size: 1 81 15 408 344
Fragments arranged by size:
408 344 81 15 1
BsiHKAI G_wGCw'C
Cuts at: 0 241 849
Size: 241 608
BsmBI CGTCTCn'nnnn_
Cuts at: 0 22 849
Size: 22 827
Bsp1286I G_dGCh'C
Cuts at: 0 241 324 849
Size: 241 83 525
Fragments arranged by size:
525 241 83
BspHI T'CATG_A
Cuts at: 0 155 849
Size: 155 694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 599 849
Size: 599 250
BsrBI CCG'CTC
Cuts at: 0 129 305 341 849
Size: 129 176 36 508
Fragments arranged by size:
508 176 129 36
BssSI C'ACGA_G
Cuts at: 0 19 849
Size: 19 830
BstAPI GCAn_nnn'nTGC
Cuts at: 0 351 849
Size: 351 498
BstXI CCAn_nnnn'nTGG
Cuts at: 0 370 849
Size: 370 479
BstYI r'GATC_y
Cuts at: 0 290 849
Size: 290 559
BtgI C'CryG_G
Cuts at: 0 183 363 452 849
Size: 183 180 89 397
Fragments arranged by size:
397 183 180 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 323 849
Size: 323 526
DraIII CAC_nnn'GTG
Cuts at: 0 105 120 849
Size: 105 15 729
Fragments arranged by size:
729 105 15
DrdI GACnn_nn'nnGTC
Cuts at: 0 36 849
Size: 36 813
EaeI y'GGCC_r
Cuts at: 0 474 849
Size: 474 375
EarI CTCTTCn'nnn_
Cuts at: 0 40 849
Size: 40 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 139 849
Size: 139 710
EcoICRI GAG'CTC
Cuts at: 0 239 849
Size: 239 610
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 97 539 849
Size: 64 33 442 310
Fragments arranged by size:
442 310 64 33
EcoO109I rG'GnC_Cy
Cuts at: 0 138 170 734 849
Size: 138 32 564 115
Fragments arranged by size:
564 138 115 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 849
Size: 454 32 363
Fragments arranged by size:
454 363 32
MslI CAynn'nnrTG
Cuts at: 0 368 424 849
Size: 368 56 425
Fragments arranged by size:
425 368 56
MspA1I CmG'CkG
Cuts at: 0 348 849
Size: 348 501
NcoI C'CATG_G
Cuts at: 0 452 849
Size: 452 397
NspI r_CATG'y
Cuts at: 0 320 468 849
Size: 320 148 381
Fragments arranged by size:
381 320 148
PasI CC'CwG_GG
Cuts at: 0 141 849
Size: 141 708
PciI A'CATG_T
Cuts at: 0 316 849
Size: 316 533
PflMI CCAn_nnn'nTGG
Cuts at: 0 458 849
Size: 458 391
PpuMI rG'GwC_Cy
Cuts at: 0 170 734 849
Size: 170 564 115
Fragments arranged by size:
564 170 115
PstI C_TGCA'G
Cuts at: 0 356 849
Size: 356 493
PvuII CAG'CTG
Cuts at: 0 348 849
Size: 348 501
SacI G_AGCT'C
Cuts at: 0 241 849
Size: 241 608
SanDI GG'GwC_CC
Cuts at: 0 734 849
Size: 734 115
SapI GCTCTTCn'nnn_
Cuts at: 0 40 849
Size: 40 809
SexAI A'CCwGG_T
Cuts at: 0 116 849
Size: 116 733
SfcI C'TryA_G
Cuts at: 0 352 849
Size: 352 497
SmaI CCC'GGG
Cuts at: 0 739 849
Size: 739 110
SmlI C'TyrA_G
Cuts at: 0 64 849
Size: 64 785
StuI AGG'CCT
Cuts at: 0 490 849
Size: 490 359
StyI C'CwwG_G
Cuts at: 0 452 849
Size: 452 397
XmaI C'CCGG_G
Cuts at: 0 737 849
Size: 737 112
ZraI GAC'GTC
Cuts at: 0 381 849
Size: 381 468
Enzymes that do cut:
AatII AcuI AflIII AleI AlwNI AvaI BanII BbsI
BcgI BciVI BglI Bme1580I BmgBI BmrI BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BssSI BstAPI BstXI
BstYI BtgI BtgZI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I Hin4I MslI MspA1I NcoI NspI
PasI PciI PflMI PpuMI PstI PvuII SacI SanDI
SapI SexAI SfcI SmaI SmlI StuI StyI XmaI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BclI BfrBI
BglII BlpI BmtI BplI Bpu10I BsaBI BseYI BsiEI
BsiWI BsmI BspEI BsrDI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI EagI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI