Restriction map for
pF1KE0094
(Linear) (Six-base) MAPSORT of: KIEE0094.seq Check: 796 from: 1 to: 1605
KIEE0094.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 0 755 1605
Size: 755 850
Acc65I G'GTAC_C
Cuts at: 0 227 1605
Size: 227 1378
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 961 1087 1141 1605
Size: 348 613 126 54 464
Fragments arranged by size:
613 464 348 126 54
AgeI A'CCGG_T
Cuts at: 0 1368 1605
Size: 1368 237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 115 574 1605
Size: 115 459 1031
Fragments arranged by size:
1031 459 115
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 230 262 1605
Size: 230 32 1343
Fragments arranged by size:
1343 230 32
AlwNI CAG_nnn'CTG
Cuts at: 0 684 852 1119 1605
Size: 684 168 267 486
Fragments arranged by size:
684 486 267 168
ApaI G_GGCC'C
Cuts at: 0 705 1605
Size: 705 900
AvaI C'yCGr_G
Cuts at: 0 43 1605
Size: 43 1562
BamHI G'GATC_C
Cuts at: 0 348 467 1605
Size: 348 119 1138
Fragments arranged by size:
1138 348 119
BanI G'GyrC_C
Cuts at: 0 227 1246 1605
Size: 227 1019 359
Fragments arranged by size:
1019 359 227
BanII G_rGCy'C
Cuts at: 0 74 275 705 1605
Size: 74 201 430 900
Fragments arranged by size:
900 430 201 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 217 1605
Size: 217 1388
BbvCI CC'TCA_GC
Cuts at: 0 818 1605
Size: 818 787
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1483 1517 1546 1580 1605
Size: 1483 34 29 34 25
Fragments arranged by size:
1483 34 34 29 25
BglI GCCn_nnn'nGGC
Cuts at: 0 907 1605
Size: 907 698
BglII A'GATC_T
Cuts at: 0 1010 1040 1349 1448 1605
Size: 1010 30 309 99 157
Fragments arranged by size:
1010 309 157 99 30
Bme1580I G_kGCm'C
Cuts at: 0 417 705 1249 1544 1605
Size: 417 288 544 295 61
Fragments arranged by size:
544 417 295 288 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 215 1605
Size: 215 1390
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 153 972 1605
Size: 153 819 633
Fragments arranged by size:
819 633 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 457 1156 1310 1555 1605
Size: 169 288 699 154 245 50
Fragments arranged by size:
699 288 245 169 154 50
Bpu10I CC'TnA_GC
Cuts at: 0 269 818 1605
Size: 269 549 787
Fragments arranged by size:
787 549 269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 544 604 1605
Size: 58 486 60 1001
Fragments arranged by size:
1001 486 60 58
BsaWI w'CCGG_w
Cuts at: 0 800 1368 1605
Size: 800 568 237
Fragments arranged by size:
800 568 237
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 748 778 1605
Size: 748 30 827
Fragments arranged by size:
827 748 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 775 1213 1605
Size: 775 438 392
Fragments arranged by size:
775 438 392
BseYI C'CCAG_C
Cuts at: 0 12 416 1172 1440 1605
Size: 12 404 756 268 165
Fragments arranged by size:
756 404 268 165 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1237 1605
Size: 1237 368
BsiEI CG_ry'CG
Cuts at: 0 27 1605
Size: 27 1578
BsiHKAI G_wGCw'C
Cuts at: 0 1037 1605
Size: 1037 568
BsmBI CGTCTCn'nnnn_
Cuts at: 0 667 1605
Size: 667 938
Bsp1286I G_dGCh'C
Cuts at: 0 74 275 417 705 1037 1249 1544 1605
Size: 74 201 142 288 332 212 295 61
Fragments arranged by size:
332 295 288 212 201 142 74 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 1605
Size: 239 1366
BsrBI CCG'CTC
Cuts at: 0 540 1029 1605
Size: 540 489 576
Fragments arranged by size:
576 540 489
BsrDI GCAATG_nn'
Cuts at: 0 290 1166 1605
Size: 290 876 439
Fragments arranged by size:
876 439 290
BsrFI r'CCGG_y
Cuts at: 0 1368 1605
Size: 1368 237
BssSI C'ACGA_G
Cuts at: 0 127 628 1543 1605
Size: 127 501 915 62
Fragments arranged by size:
915 501 127 62
BstAPI GCAn_nnn'nTGC
Cuts at: 0 687 1548 1605
Size: 687 861 57
Fragments arranged by size:
861 687 57
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1221 1605
Size: 1221 384
BstYI r'GATC_y
Cuts at: 0 348 467 1010 1040 1349 1448 1605
Size: 348 119 543 30 309 99 157
Fragments arranged by size:
543 348 309 157 119 99 30
BtgI C'CryG_G
Cuts at: 0 176 1605
Size: 176 1429
ClaI AT'CG_AT
Cuts at: 0 1301 1605
Size: 1301 304
EaeI y'GGCC_r
Cuts at: 0 908 1004 1017 1322 1460 1508 1605
Size: 908 96 13 305 138 48 97
Fragments arranged by size:
908 305 138 97 96 48 13
EarI CTCTTCn'nnn_
Cuts at: 0 104 568 1063 1117 1605
Size: 104 464 495 54 488
Fragments arranged by size:
495 488 464 104 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1247 1605
Size: 1247 358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 348 457 961 1087 1141 1156 1310
Size: 169 179 109 504 126 54 15 154
Cuts at: 1310 1555 1605
Size: 245 50
Fragments arranged by size:
504 245 179 169 154 126 109 54
50 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 222 1605
Size: 222 1383
EcoO109I rG'GnC_Cy
Cuts at: 0 39 701 702 828 1605
Size: 39 662 1 126 777
Fragments arranged by size:
777 662 126 39 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 234 378 1605
Size: 234 144 1227
Fragments arranged by size:
1227 234 144
FspI TGC'GCA
Cuts at: 0 874 1605
Size: 874 731
HaeII r_GCGC'y
Cuts at: 0 1394 1605
Size: 1394 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 583 615 1283 1315 1605
Size: 583 32 668 32 290
Fragments arranged by size:
668 583 290 32 32
HincII GTy'rAC
Cuts at: 0 546 996 1605
Size: 546 450 609
Fragments arranged by size:
609 546 450
KpnI G_GTAC'C
Cuts at: 0 231 1605
Size: 231 1374
MscI TGG'CCA
Cuts at: 0 910 1324 1510 1605
Size: 910 414 186 95
Fragments arranged by size:
910 414 186 95
MslI CAynn'nnrTG
Cuts at: 0 295 1219 1274 1605
Size: 295 924 55 331
Fragments arranged by size:
924 331 295 55
MspA1I CmG'CkG
Cuts at: 0 8 517 684 1086 1119 1209 1605
Size: 8 509 167 402 33 90 396
Fragments arranged by size:
509 402 396 167 90 33 8
NcoI C'CATG_G
Cuts at: 0 176 1605
Size: 176 1429
NspI r_CATG'y
Cuts at: 0 1155 1485 1605
Size: 1155 330 120
Fragments arranged by size:
1155 330 120
PasI CC'CwG_GG
Cuts at: 0 328 1605
Size: 328 1277
PflMI CCAn_nnn'nTGG
Cuts at: 0 579 1179 1605
Size: 579 600 426
Fragments arranged by size:
600 579 426
PpuMI rG'GwC_Cy
Cuts at: 0 39 828 1605
Size: 39 789 777
Fragments arranged by size:
789 777 39
PspOMI G'GGCC_C
Cuts at: 0 701 1605
Size: 701 904
PstI C_TGCA'G
Cuts at: 0 1091 1605
Size: 1091 514
PvuII CAG'CTG
Cuts at: 0 8 517 684 1086 1605
Size: 8 509 167 402 519
Fragments arranged by size:
519 509 402 167 8
SapI GCTCTTCn'nnn_
Cuts at: 0 104 568 1117 1605
Size: 104 464 549 488
Fragments arranged by size:
549 488 464 104
ScaI AGT'ACT
Cuts at: 0 199 1605
Size: 199 1406
SfcI C'TryA_G
Cuts at: 0 1087 1605
Size: 1087 518
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 907 1605
Size: 907 698
SgrAI Cr'CCGG_yG
Cuts at: 0 1368 1605
Size: 1368 237
SmlI C'TyrA_G
Cuts at: 0 73 523 619 1605
Size: 73 450 96 986
Fragments arranged by size:
986 450 96 73
SphI G_CATG'C
Cuts at: 0 1485 1605
Size: 1485 120
StyI C'CwwG_G
Cuts at: 0 104 176 716 911 1605
Size: 104 72 540 195 694
Fragments arranged by size:
694 540 195 104 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1305 1605
Size: 1305 300
TatI w'GTAC_w
Cuts at: 0 197 1605
Size: 197 1408
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 452 484 1605
Size: 452 32 1121
Fragments arranged by size:
1121 452 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 183 202 1045 1407 1437 1605
Size: 183 19 843 362 30 168
Fragments arranged by size:
843 362 183 168 30 19
XmnI GAAnn'nnTTC
Cuts at: 0 361 403 1605
Size: 361 42 1202
Fragments arranged by size:
1202 361 42
Enzymes that do cut:
AccI Acc65I AcuI AgeI AlfI AloI AlwNI ApaI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BglI
BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI
BtgI ClaI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII KpnI MscI MslI
MspA1I NcoI NspI PasI PflMI PpuMI PspOMI PstI
PvuII SapI ScaI SfcI SfiI SgrAI SmlI SphI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AhdI AleI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BciVI BclI BfrBI BlpI BmgBI BmtI BsaI BsaAI
BsaBI BsaHI BsiWI BsmI BspEI BspHI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI CspCI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FseI FspAI
HindIII HpaI KasI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SfoI SgfI SmaI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI ZraI