Restriction map for
pF1KE0090
(Linear) (Six-base) MAPSORT of: KIEE0090.seq Check: 3646 from: 1 to: 1449
KIEE0090.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 0 1193 1449
Size: 1193 256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1316 1449
Size: 1316 133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1199 1449
Size: 1199 250
AlwNI CAG_nnn'CTG
Cuts at: 0 98 1449
Size: 98 1351
ApoI r'AATT_y
Cuts at: 0 679 989 1303 1449
Size: 679 310 314 146
Fragments arranged by size:
679 314 310 146
AvaI C'yCGr_G
Cuts at: 0 554 1449
Size: 554 895
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 112 145 715 748 1449
Size: 112 33 570 33 701
Fragments arranged by size:
701 570 112 33 33
BamHI G'GATC_C
Cuts at: 0 794 1449
Size: 794 655
BanII G_rGCy'C
Cuts at: 0 935 1449
Size: 935 514
BbsI GAAGACnn'nnnn_
Cuts at: 0 24 1219 1449
Size: 24 1195 230
Fragments arranged by size:
1195 230 24
BclI T'GATC_A
Cuts at: 0 281 1449
Size: 281 1168
BfrBI ATG'CAT
Cuts at: 0 574 1449
Size: 574 875
BglI GCCn_nnn'nGGC
Cuts at: 0 439 1449
Size: 439 1010
Bme1580I G_kGCm'C
Cuts at: 0 1072 1449
Size: 1072 377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 465 605 876 981 1449
Size: 120 345 140 271 105 468
Fragments arranged by size:
468 345 271 140 120 105
BsaAI yAC'GTr
Cuts at: 0 1170 1449
Size: 1170 279
BsaBI GATnn'nnATC
Cuts at: 0 536 1449
Size: 536 913
BsaWI w'CCGG_w
Cuts at: 0 950 1449
Size: 950 499
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 614 1124 1449
Size: 614 510 325
Fragments arranged by size:
614 510 325
BseYI C'CCAG_C
Cuts at: 0 921 997 1022 1449
Size: 921 76 25 427
Fragments arranged by size:
921 427 76 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 1449
Size: 1192 257
BsiHKAI G_wGCw'C
Cuts at: 0 11 935 1449
Size: 11 924 514
Fragments arranged by size:
924 514 11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 957 1449
Size: 957 492
Bsp1286I G_dGCh'C
Cuts at: 0 11 935 1072 1449
Size: 11 924 137 377
Fragments arranged by size:
924 377 137 11
BspHI T'CATG_A
Cuts at: 0 675 1449
Size: 675 774
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 960 1449
Size: 960 489
BstYI r'GATC_y
Cuts at: 0 794 1449
Size: 794 655
Bsu36I CC'TnA_GG
Cuts at: 0 1198 1449
Size: 1198 251
BtsI GCAGTG_nn'
Cuts at: 0 59 428 1070 1449
Size: 59 369 642 379
Fragments arranged by size:
642 379 369 59
ClaI AT'CG_AT
Cuts at: 0 1328 1449
Size: 1328 121
EaeI y'GGCC_r
Cuts at: 0 314 684 1320 1449
Size: 314 370 636 129
Fragments arranged by size:
636 370 314 129
EarI CTCTTCn'nnn_
Cuts at: 0 334 946 1449
Size: 334 612 503
Fragments arranged by size:
612 503 334
EcoICRI GAG'CTC
Cuts at: 0 933 1449
Size: 933 516
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 465 605 876 981 1316 1449
Size: 120 345 140 271 105 335 133
Fragments arranged by size:
345 335 271 140 133 120 105
EcoNI CCTnn'n_nnAGG
Cuts at: 0 90 121 587 1449
Size: 90 31 466 862
Fragments arranged by size:
862 466 90 31
EcoO109I rG'GnC_Cy
Cuts at: 0 95 1090 1129 1449
Size: 95 995 39 320
Fragments arranged by size:
995 320 95 39
EcoRI G'AATT_C
Cuts at: 0 989 1449
Size: 989 460
EcoRV GAT'ATC
Cuts at: 0 506 1449
Size: 506 943
HaeII r_GCGC'y
Cuts at: 0 455 1449
Size: 455 994
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 755 787 880 912 1449
Size: 755 32 93 32 537
Fragments arranged by size:
755 537 93 32 32
MscI TGG'CCA
Cuts at: 0 316 686 1322 1449
Size: 316 370 636 127
Fragments arranged by size:
636 370 316 127
NsiI A_TGCA'T
Cuts at: 0 576 1449
Size: 576 873
PasI CC'CwG_GG
Cuts at: 0 693 1449
Size: 693 756
PflMI CCAn_nnn'nTGG
Cuts at: 0 98 547 693 1449
Size: 98 449 146 756
Fragments arranged by size:
756 449 146 98
PfoI T'CCnGG_A
Cuts at: 0 582 1435 1449
Size: 582 853 14
Fragments arranged by size:
853 582 14
PmlI CAC'GTG
Cuts at: 0 1170 1449
Size: 1170 279
PpuMI rG'GwC_Cy
Cuts at: 0 95 1090 1129 1449
Size: 95 995 39 320
Fragments arranged by size:
995 320 95 39
PsiI TTA'TAA
Cuts at: 0 743 1449
Size: 743 706
PstI C_TGCA'G
Cuts at: 0 66 1449
Size: 66 1383
SacI G_AGCT'C
Cuts at: 0 935 1449
Size: 935 514
SapI GCTCTTCn'nnn_
Cuts at: 0 946 1449
Size: 946 503
SfcI C'TryA_G
Cuts at: 0 62 1242 1337 1449
Size: 62 1180 95 112
Fragments arranged by size:
1180 112 95 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 439 1449
Size: 439 1010
SspI AAT'ATT
Cuts at: 0 142 1449
Size: 142 1307
StuI AGG'CCT
Cuts at: 0 442 593 1449
Size: 442 151 856
Fragments arranged by size:
856 442 151
StyI C'CwwG_G
Cuts at: 0 1162 1449
Size: 1162 287
TatI w'GTAC_w
Cuts at: 0 746 1449
Size: 746 703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 517 549 853 885 1449
Size: 517 32 304 32 564
Fragments arranged by size:
564 517 304 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 195 823 1449
Size: 195 628 626
Fragments arranged by size:
628 626 195
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApoI AvaI BaeI BamHI
BanII BbsI BclI BfrBI BglI Bme1580I BpmI BsaAI
BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BstYI Bsu36I BtsI ClaI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I
MscI NsiI PasI PflMI PfoI PmlI PpuMI PsiI
PstI SacI SapI SfcI SfiI SspI StuI StyI
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BanI BbeI BbvCI BcgI BciVI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaHI
BsaXI BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgI BtgZI CspCI DraI DraIII DrdI EagI EciI
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PacI
PciI PmeI PpiI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
ZraI