Restriction map for
pF1KE0083
(Linear) (Six-base) MAPSORT of: KIEE0083.seq Check: 5188 from: 1 to: 1575
KIEE0083.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1046 1575
Size: 772 274 529
Fragments arranged by size:
772 529 274
AflIII A'CryG_T
Cuts at: 0 920 1575
Size: 920 655
AleI CACnn'nnGTG
Cuts at: 0 1316 1575
Size: 1316 259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 137 1575
Size: 137 1438
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1364 1396 1575
Size: 1364 32 179
Fragments arranged by size:
1364 179 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1485 1575
Size: 1485 90
ApoI r'AATT_y
Cuts at: 0 1520 1575
Size: 1520 55
AseI AT'TA_AT
Cuts at: 0 385 1575
Size: 385 1190
AvaI C'yCGr_G
Cuts at: 0 543 1575
Size: 543 1032
AvrII C'CTAG_G
Cuts at: 0 498 1575
Size: 498 1077
BanII G_rGCy'C
Cuts at: 0 542 1575
Size: 542 1033
BbsI GAAGACnn'nnnn_
Cuts at: 0 1496 1575
Size: 1496 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1226 1260 1575
Size: 1226 34 315
Fragments arranged by size:
1226 315 34
BclI T'GATC_A
Cuts at: 0 306 1575
Size: 306 1269
BlpI GC'TnA_GC
Cuts at: 0 844 1575
Size: 844 731
Bme1580I G_kGCm'C
Cuts at: 0 1474 1575
Size: 1474 101
BmgBI CAC'GTC
Cuts at: 0 241 1575
Size: 241 1334
BmrI ACTGGGnnnn_n'
Cuts at: 0 123 457 1575
Size: 123 334 1118
Fragments arranged by size:
1118 334 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1317 1575
Size: 28 1289 258
Fragments arranged by size:
1289 258 28
Bpu10I CC'TnA_GC
Cuts at: 0 1475 1575
Size: 1475 100
BsaBI GATnn'nnATC
Cuts at: 0 983 1575
Size: 983 592
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 335 601 1175 1272 1519 1575
Size: 335 266 574 97 247 56
Fragments arranged by size:
574 335 266 247 97 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 873 1532 1575
Size: 873 659 43
Fragments arranged by size:
873 659 43
BsiHKAI G_wGCw'C
Cuts at: 0 542 689 1575
Size: 542 147 886
Fragments arranged by size:
886 542 147
BsmI GAATG_Cn'
Cuts at: 0 703 863 1575
Size: 703 160 712
Fragments arranged by size:
712 703 160
Bsp1286I G_dGCh'C
Cuts at: 0 542 689 1474 1575
Size: 542 147 785 101
Fragments arranged by size:
785 542 147 101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 839 1575
Size: 839 736
BsrBI CCG'CTC
Cuts at: 0 12 1575
Size: 12 1563
BsrDI GCAATG_nn'
Cuts at: 0 162 1575
Size: 162 1413
BstAPI GCAn_nnn'nTGC
Cuts at: 0 923 1575
Size: 923 652
BstBI TT'CG_AA
Cuts at: 0 147 816 855 1575
Size: 147 669 39 720
Fragments arranged by size:
720 669 147 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1318 1575
Size: 1318 257
BstYI r'GATC_y
Cuts at: 0 480 1575
Size: 480 1095
BtgI C'CryG_G
Cuts at: 0 71 1367 1575
Size: 71 1296 208
Fragments arranged by size:
1296 208 71
DraI TTT'AAA
Cuts at: 0 512 1575
Size: 512 1063
EaeI y'GGCC_r
Cuts at: 0 776 1575
Size: 776 799
EarI CTCTTCn'nnn_
Cuts at: 0 428 1575
Size: 428 1147
EcoICRI GAG'CTC
Cuts at: 0 540 1575
Size: 540 1035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 772 1046 1317 1575
Size: 28 744 274 271 258
Fragments arranged by size:
744 274 271 258 28
EcoO109I rG'GnC_Cy
Cuts at: 0 1482 1575
Size: 1482 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 726 917 1575
Size: 726 191 658
Fragments arranged by size:
726 658 191
HaeII r_GCGC'y
Cuts at: 0 1281 1575
Size: 1281 294
HincII GTy'rAC
Cuts at: 0 257 1575
Size: 257 1318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1226 1575
Size: 1226 349
MscI TGG'CCA
Cuts at: 0 778 1575
Size: 778 797
MslI CAynn'nnrTG
Cuts at: 0 294 692 1316 1575
Size: 294 398 624 259
Fragments arranged by size:
624 398 294 259
MspA1I CmG'CkG
Cuts at: 0 1298 1575
Size: 1298 277
NcoI C'CATG_G
Cuts at: 0 1367 1575
Size: 1367 208
NspI r_CATG'y
Cuts at: 0 924 1575
Size: 924 651
PciI A'CATG_T
Cuts at: 0 920 1575
Size: 920 655
PstI C_TGCA'G
Cuts at: 0 1230 1575
Size: 1230 345
SacI G_AGCT'C
Cuts at: 0 542 1575
Size: 542 1033
SapI GCTCTTCn'nnn_
Cuts at: 0 428 1575
Size: 428 1147
ScaI AGT'ACT
Cuts at: 0 50 1575
Size: 50 1525
SfcI C'TryA_G
Cuts at: 0 1226 1575
Size: 1226 349
SspI AAT'ATT
Cuts at: 0 102 466 1575
Size: 102 364 1109
Fragments arranged by size:
1109 364 102
StuI AGG'CCT
Cuts at: 0 1330 1575
Size: 1330 245
StyI C'CwwG_G
Cuts at: 0 498 973 1367 1575
Size: 498 475 394 208
Fragments arranged by size:
498 475 394 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 12 1508 1575
Size: 12 1496 67
Fragments arranged by size:
1496 67 12
TatI w'GTAC_w
Cuts at: 0 48 1101 1575
Size: 48 1053 474
Fragments arranged by size:
1053 474 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 794 826 1575
Size: 794 32 749
Fragments arranged by size:
794 749 32
XmnI GAAnn'nnTTC
Cuts at: 0 735 1575
Size: 735 840
Enzymes that do cut:
AcuI AflIII AleI AlfI AloI AlwNI ApoI AseI
AvaI AvrII BanII BbsI BcgI BclI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaBI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BstAPI BstBI BstXI
BstYI BtgI DraI EaeI EarI EcoICRI Eco57MI EcoO109I
FalI HaeII HincII MmeI MscI MslI MspA1I NcoI
NspI PciI PstI SacI SapI ScaI SfcI SspI
StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI ApaI ApaLI AscI AsiSI BaeI BamHI BanI
BbeI BbvCI BciVI BfrBI BglI BglII BmtI BplI
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI
BsiWI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI