Restriction map for
pF1KE0082
(Linear) (Six-base) MAPSORT of: KIEE0082.seq Check: 3852 from: 1 to: 1986
KIEE0082.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 101 1986
Size: 101 1885
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 747 853 1474 1986
Size: 298 449 106 621 512
Fragments arranged by size:
621 512 449 298 106
AflIII A'CryG_T
Cuts at: 0 262 1986
Size: 262 1724
AleI CACnn'nnGTG
Cuts at: 0 1562 1986
Size: 1562 424
ApaLI G'TGCA_C
Cuts at: 0 1665 1986
Size: 1665 321
ApoI r'AATT_y
Cuts at: 0 68 797 965 1093 1177 1345 1429 1553
Size: 68 729 168 128 84 168 84 124
Cuts at: 1553 1597 1695 1765 1889 1947 1986
Size: 44 98 70 124 58 39
Fragments arranged by size:
729 168 168 128 124 124 98 84
84 70 68 58 44 39
BanI G'GyrC_C
Cuts at: 0 171 1712 1986
Size: 171 1541 274
Fragments arranged by size:
1541 274 171
BfrBI ATG'CAT
Cuts at: 0 446 1986
Size: 446 1540
BglII A'GATC_T
Cuts at: 0 480 1986
Size: 480 1506
BlpI GC'TnA_GC
Cuts at: 0 299 1986
Size: 299 1687
Bme1580I G_kGCm'C
Cuts at: 0 1669 1986
Size: 1669 317
BmgBI CAC'GTC
Cuts at: 0 36 1986
Size: 36 1950
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 388 1986
Size: 388 1598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 743 827 995 1079 1163 1247 1331
Size: 133 610 84 168 84 84 84 84
Cuts at: 1331 1415 1499 1835 1851 1919 1986
Size: 84 84 336 16 68 67
Fragments arranged by size:
610 336 168 133 84 84 84 84
84 84 84 68 67 16
BsaI GGTCTCn'nnnn_
Cuts at: 0 1899 1986
Size: 1899 87
BsaBI GATnn'nnATC
Cuts at: 0 479 1544 1986
Size: 479 1065 442
Fragments arranged by size:
1065 479 442
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 181 1986
Size: 73 108 1805
Fragments arranged by size:
1805 108 73
BseYI C'CCAG_C
Cuts at: 0 248 1986
Size: 248 1738
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 328 558 678 846 1095 1854 1938
Size: 79 249 230 120 168 249 759 84
Cuts at: 1938 1986
Size: 48
Fragments arranged by size:
759 249 249 230 168 120 84 79
48
BsiHKAI G_wGCw'C
Cuts at: 0 623 1669 1986
Size: 623 1046 317
Fragments arranged by size:
1046 623 317
BsmI GAATG_Cn'
Cuts at: 0 1165 1333 1417 1501 1986
Size: 1165 168 84 84 485
Fragments arranged by size:
1165 485 168 84 84
Bsp1286I G_dGCh'C
Cuts at: 0 623 1669 1986
Size: 623 1046 317
Fragments arranged by size:
1046 623 317
BspHI T'CATG_A
Cuts at: 0 1797 1986
Size: 1797 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 101 350 1986
Size: 101 249 1636
Fragments arranged by size:
1636 249 101
BsrDI GCAATG_nn'
Cuts at: 0 190 1002 1986
Size: 190 812 984
Fragments arranged by size:
984 812 190
BsrGI T'GTAC_A
Cuts at: 0 1019 1447 1986
Size: 1019 428 539
Fragments arranged by size:
1019 539 428
BstEII G'GTnAC_C
Cuts at: 0 955 1986
Size: 955 1031
BstYI r'GATC_y
Cuts at: 0 480 1986
Size: 480 1506
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 116 1986
Size: 116 1870
BtsI GCAGTG_nn'
Cuts at: 0 666 834 1422 1842 1926 1986
Size: 666 168 588 420 84 60
Fragments arranged by size:
666 588 420 168 84 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 984 1019 1152 1187 1320 1355 1986
Size: 984 35 133 35 133 35 631
Fragments arranged by size:
984 631 133 133 35 35 35
DraI TTT'AAA
Cuts at: 0 221 1986
Size: 221 1765
DraIII CAC_nnn'GTG
Cuts at: 0 1673 1986
Size: 1673 313
EaeI y'GGCC_r
Cuts at: 0 38 1986
Size: 38 1948
EarI CTCTTCn'nnn_
Cuts at: 0 503 1450 1926 1986
Size: 503 947 476 60
Fragments arranged by size:
947 503 476 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 298 743 747 827 853 995 1079
Size: 133 165 445 4 80 26 142 84
Cuts at: 1079 1163 1247 1331 1415 1474 1499 1835 1851
Size: 84 84 84 84 59 25 336 16
Cuts at: 1851 1919 1986
Size: 68 67
Fragments arranged by size:
445 336 165 142 133 84 84 84
84 84 80 68 67 59 26 25
16 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 937 1986
Size: 10 927 1049
Fragments arranged by size:
1049 927 10
EcoRI G'AATT_C
Cuts at: 0 68 1553 1889 1986
Size: 68 1485 336 97
Fragments arranged by size:
1485 336 97 68
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 865 1986
Size: 865 1121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1896 1928 1986
Size: 1896 32 58
Fragments arranged by size:
1896 58 32
HindIII A'AGCT_T
Cuts at: 0 334 842 1986
Size: 334 508 1144
Fragments arranged by size:
1144 508 334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 1986
Size: 716 1270
MscI TGG'CCA
Cuts at: 0 40 1986
Size: 40 1946
MslI CAynn'nnrTG
Cuts at: 0 101 445 1562 1986
Size: 101 344 1117 424
Fragments arranged by size:
1117 424 344 101
MspA1I CmG'CkG
Cuts at: 0 572 1109 1445 1986
Size: 572 537 336 541
Fragments arranged by size:
572 541 537 336
NsiI A_TGCA'T
Cuts at: 0 448 1986
Size: 448 1538
NspI r_CATG'y
Cuts at: 0 266 446 450 1986
Size: 266 180 4 1536
Fragments arranged by size:
1536 266 180 4
PciI A'CATG_T
Cuts at: 0 262 1986
Size: 262 1724
PsiI TTA'TAA
Cuts at: 0 737 1986
Size: 737 1249
PvuII CAG'CTG
Cuts at: 0 572 1109 1445 1986
Size: 572 537 336 541
Fragments arranged by size:
572 541 537 336
SapI GCTCTTCn'nnn_
Cuts at: 0 503 1986
Size: 503 1483
SfcI C'TryA_G
Cuts at: 0 194 1986
Size: 194 1792
SphI G_CATG'C
Cuts at: 0 450 1986
Size: 450 1536
StuI AGG'CCT
Cuts at: 0 396 544 648 732 816 900 928 1068
Size: 396 148 104 84 84 84 28 140
Cuts at: 1068 1404 1572 1986
Size: 336 168 414
Fragments arranged by size:
414 396 336 168 148 140 104 84
84 84 28
StyI C'CwwG_G
Cuts at: 0 158 895 1986
Size: 158 737 1091
Fragments arranged by size:
1091 737 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 253 497 592 1986
Size: 253 244 95 1394
Fragments arranged by size:
1394 253 244 95
TatI w'GTAC_w
Cuts at: 0 1019 1447 1986
Size: 1019 428 539
Fragments arranged by size:
1019 539 428
XmnI GAAnn'nnTTC
Cuts at: 0 928 1986
Size: 928 1058
Enzymes that do cut:
AarI AcuI AflIII AleI ApaLI ApoI BanI BfrBI
BglII BlpI Bme1580I BmgBI BplI BpmI BsaI BsaBI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrGI BstEII BstYI BtgZI BtsI CspCI DraI
DraIII EaeI EarI Eco57MI EcoNI EcoRI FalI Hin4I
HindIII MmeI MscI MslI MspA1I NsiI NspI PciI
PsiI PvuII SapI SfcI SphI StuI StyI TaqII
TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BmrI BmtI Bpu10I BpuEI BsaAI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgI ClaI DrdI EagI EciI EcoICRI EcoO109I EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI