Restriction map for
pF1KE0078
(Linear) (Six-base) MAPSORT of: KIEE0078.seq Check: 5819 from: 1 to: 2556
KIEE0078.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 795 2346 2438 2556
Size: 795 1551 92 118
Fragments arranged by size:
1551 795 118 92
AatII G_ACGT'C
Cuts at: 0 776 2556
Size: 776 1780
AccI GT'mk_AC
Cuts at: 0 1178 2556
Size: 1178 1378
Acc65I G'GTAC_C
Cuts at: 0 2093 2556
Size: 2093 463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 1207 1387 1468 2556
Size: 453 754 180 81 1088
Fragments arranged by size:
1088 754 453 180 81
AfeI AGC'GCT
Cuts at: 0 1105 2556
Size: 1105 1451
AflIII A'CryG_T
Cuts at: 0 1286 1463 2556
Size: 1286 177 1093
Fragments arranged by size:
1286 1093 177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 532 1147 2556
Size: 532 615 1409
Fragments arranged by size:
1409 615 532
AlwNI CAG_nnn'CTG
Cuts at: 0 32 666 1080 1209 1278 2556
Size: 32 634 414 129 69 1278
Fragments arranged by size:
1278 634 414 129 69 32
ApaI G_GGCC'C
Cuts at: 0 10 63 431 1814 2036 2500 2556
Size: 10 53 368 1383 222 464 56
Fragments arranged by size:
1383 464 368 222 56 53 10
ApaLI G'TGCA_C
Cuts at: 0 183 829 2085 2158 2556
Size: 183 646 1256 73 398
Fragments arranged by size:
1256 646 398 183 73
AvaI C'yCGr_G
Cuts at: 0 271 527 1803 2476 2556
Size: 271 256 1276 673 80
Fragments arranged by size:
1276 673 271 256 80
BanI G'GyrC_C
Cuts at: 0 726 982 2093 2386 2556
Size: 726 256 1111 293 170
Fragments arranged by size:
1111 726 293 256 170
BanII G_rGCy'C
Cuts at: 0 10 39 63 431 446 1390 1814 2036
Size: 10 29 24 368 15 944 424 222
Cuts at: 2036 2468 2500 2556
Size: 432 32 56
Fragments arranged by size:
944 432 424 368 222 56 32 29
24 15 10
BbeI G_GCGC'C
Cuts at: 0 2390 2556
Size: 2390 166
BbsI GAAGACnn'nnnn_
Cuts at: 0 1021 2556
Size: 1021 1535
BbvCI CC'TCA_GC
Cuts at: 0 25 1855 2556
Size: 25 1830 701
Fragments arranged by size:
1830 701 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 690 724 2556
Size: 690 34 1832
Fragments arranged by size:
1832 690 34
BglI GCCn_nnn'nGGC
Cuts at: 0 142 662 901 1514 2218 2264 2305 2556
Size: 142 520 239 613 704 46 41 251
Fragments arranged by size:
704 613 520 251 239 142 46 41
BglII A'GATC_T
Cuts at: 0 1970 2556
Size: 1970 586
BlpI GC'TnA_GC
Cuts at: 0 520 1726 2556
Size: 520 1206 830
Fragments arranged by size:
1206 830 520
Bme1580I G_kGCm'C
Cuts at: 0 10 63 187 219 431 563 833 958
Size: 10 53 124 32 212 132 270 125
Cuts at: 958 987 1397 1814 2036 2089 2162 2242 2283
Size: 29 410 417 222 53 73 80 41
Cuts at: 2283 2339 2500 2556
Size: 56 161 56
Fragments arranged by size:
417 410 270 222 212 161 132 125
124 80 73 56 56 53 53 41
32 29 10
BmgBI CAC'GTC
Cuts at: 0 1092 1410 1466 2556
Size: 1092 318 56 1090
Fragments arranged by size:
1092 1090 318 56
BmrI ACTGGGnnnn_n'
Cuts at: 0 630 999 2556
Size: 630 369 1557
Fragments arranged by size:
1557 630 369
BmtI G_CTAG'C
Cuts at: 0 509 2556
Size: 509 2047
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 958 1102 1300 2556
Size: 958 144 198 1256
Fragments arranged by size:
1256 958 198 144
Bpu10I CC'TnA_GC
Cuts at: 0 25 667 1855 1942 2556
Size: 25 642 1188 87 614
Fragments arranged by size:
1188 642 614 87 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 526 1679 2331 2556
Size: 526 1153 652 225
Fragments arranged by size:
1153 652 526 225
BsaAI yAC'GTr
Cuts at: 0 105 305 1011 1308 2556
Size: 105 200 706 297 1248
Fragments arranged by size:
1248 706 297 200 105
BsaHI Gr'CG_yC
Cuts at: 0 773 2387 2556
Size: 773 1614 169
Fragments arranged by size:
1614 773 169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1273 1303 2556
Size: 1273 30 1253
Fragments arranged by size:
1273 1253 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 186 256 1099 2556
Size: 154 32 70 843 1457
Fragments arranged by size:
1457 843 154 70 32
BseYI C'CCAG_C
Cuts at: 0 3 108 169 334 562 756 986 1737
Size: 3 105 61 165 228 194 230 751
Cuts at: 1737 1882 1904 2000 2556
Size: 145 22 96 556
Fragments arranged by size:
751 556 230 228 194 165 145 105
96 61 22 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 595 763 787 823 976 1195 1596
Size: 28 567 168 24 36 153 219 401
Cuts at: 1596 2374 2398 2556
Size: 778 24 158
Fragments arranged by size:
778 567 401 219 168 158 153 36
28 24 24
BsiEI CG_ry'CG
Cuts at: 0 570 587 657 1967 2556
Size: 570 17 70 1310 589
Fragments arranged by size:
1310 589 570 70 17
BsiHKAI G_wGCw'C
Cuts at: 0 187 310 446 833 1498 1948 2089 2162
Size: 187 123 136 387 665 450 141 73
Cuts at: 2162 2556
Size: 394
Fragments arranged by size:
665 450 394 387 187 141 136 123
73
Bsp1286I G_dGCh'C
Cuts at: 0 10 39 63 187 219 310 431 446
Size: 10 29 24 124 32 91 121 15
Cuts at: 446 563 833 958 987 1390 1397 1498 1814
Size: 117 270 125 29 403 7 101 316
Cuts at: 1814 1948 2036 2089 2162 2242 2283 2339 2468
Size: 134 88 53 73 80 41 56 129
Cuts at: 2468 2500 2556
Size: 32 56
Fragments arranged by size:
403 316 270 134 129 125 124 121
117 101 91 88 80 73 56 56
53 41 32 32 29 29 24 15
10 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 795 1406 1661 2346 2438 2453 2556
Size: 795 611 255 685 92 15 103
Fragments arranged by size:
795 685 611 255 103 92 15
BsrBI CCG'CTC
Cuts at: 0 1929 2556
Size: 1929 627
BsrFI r'CCGG_y
Cuts at: 0 457 656 1586 1698 2009 2556
Size: 457 199 930 112 311 547
Fragments arranged by size:
930 547 457 311 199 112
BsrGI T'GTAC_A
Cuts at: 0 1289 2556
Size: 1289 1267
BssSI C'ACGA_G
Cuts at: 0 715 994 2556
Size: 715 279 1562
Fragments arranged by size:
1562 715 279
BstAPI GCAn_nnn'nTGC
Cuts at: 0 810 1658 2139 2556
Size: 810 848 481 417
Fragments arranged by size:
848 810 481 417
BstEII G'GTnAC_C
Cuts at: 0 453 2556
Size: 453 2103
BstXI CCAn_nnnn'nTGG
Cuts at: 0 231 2070 2556
Size: 231 1839 486
Fragments arranged by size:
1839 486 231
BstYI r'GATC_y
Cuts at: 0 261 1451 1970 2556
Size: 261 1190 519 586
Fragments arranged by size:
1190 586 519 261
Bsu36I CC'TnA_GG
Cuts at: 0 1236 1432 2365 2556
Size: 1236 196 933 191
Fragments arranged by size:
1236 933 196 191
BtgI C'CryG_G
Cuts at: 0 236 449 590 689 2144 2283 2556
Size: 236 213 141 99 1455 139 273
Fragments arranged by size:
1455 273 236 213 141 139 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 357 693 1590 2556
Size: 357 336 897 966
Fragments arranged by size:
966 897 357 336
BtsI GCAGTG_nn'
Cuts at: 0 1120 1510 2160 2556
Size: 1120 390 650 396
Fragments arranged by size:
1120 650 396 390
DraIII CAC_nnn'GTG
Cuts at: 0 786 1410 2556
Size: 786 624 1146
Fragments arranged by size:
1146 786 624
EaeI y'GGCC_r
Cuts at: 0 221 233 323 350 584 626 654 686
Size: 221 12 90 27 234 42 28 32
Cuts at: 686 893 1031 1623 1875 1964 2060 2345 2556
Size: 207 138 592 252 89 96 285 211
Fragments arranged by size:
592 285 252 234 221 211 207 138
96 90 89 42 32 28 27 12
EagI C'GGCC_G
Cuts at: 0 584 654 1964 2556
Size: 584 70 1310 592
Fragments arranged by size:
1310 592 584 70
EarI CTCTTCn'nnn_
Cuts at: 0 853 1226 1957 2556
Size: 853 373 731 599
Fragments arranged by size:
853 731 599 373
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1654 2556
Size: 1654 902
EcoICRI GAG'CTC
Cuts at: 0 444 2556
Size: 444 2112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 958 1102 1207 1300 1387 1468 2556
Size: 453 505 144 105 93 87 81 1088
Fragments arranged by size:
1088 505 453 144 105 93 87 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 130 352 781 2556
Size: 130 222 429 1775
Fragments arranged by size:
1775 429 222 130
EcoO109I rG'GnC_Cy
Cuts at: 0 7 59 60 428 663 720 1206 1254
Size: 7 52 1 368 235 57 486 48
Cuts at: 1254 1810 1811 2032 2033 2496 2497 2556
Size: 556 1 221 1 463 1 59
Fragments arranged by size:
556 486 463 368 235 221 59 57
52 48 7 1 1 1 1
HaeII r_GCGC'y
Cuts at: 0 527 1107 1446 1738 2155 2390 2556
Size: 527 580 339 292 417 235 166
Fragments arranged by size:
580 527 417 339 292 235 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 1621 1653 2556
Size: 292 32 1297 32 903
Fragments arranged by size:
1297 903 292 32 32
KasI G'GCGC_C
Cuts at: 0 2386 2556
Size: 2386 170
KpnI G_GTAC'C
Cuts at: 0 2097 2556
Size: 2097 459
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 2556
Size: 239 2317
MscI TGG'CCA
Cuts at: 0 223 325 352 895 1033 1625 1877 2062
Size: 223 102 27 543 138 592 252 185
Cuts at: 2062 2347 2556
Size: 285 209
Fragments arranged by size:
592 543 285 252 223 209 185 138
102 27
MslI CAynn'nnrTG
Cuts at: 0 229 2068 2556
Size: 229 1839 488
Fragments arranged by size:
1839 488 229
MspA1I CmG'CkG
Cuts at: 0 579 592 691 732 990 1186 1323 1340
Size: 579 13 99 41 258 196 137 17
Cuts at: 1340 1457 2556
Size: 117 1099
Fragments arranged by size:
1099 579 258 196 137 117 99 41
17 13
NaeI GCC'GGC
Cuts at: 0 658 1700 2556
Size: 658 1042 856
Fragments arranged by size:
1042 856 658
NarI GG'CG_CC
Cuts at: 0 2387 2556
Size: 2387 169
NcoI C'CATG_G
Cuts at: 0 449 2556
Size: 449 2107
NgoMIV G'CCGG_C
Cuts at: 0 656 1698 2556
Size: 656 1042 858
Fragments arranged by size:
1042 858 656
NheI G'CTAG_C
Cuts at: 0 505 2556
Size: 505 2051
NspI r_CATG'y
Cuts at: 0 1290 2139 2556
Size: 1290 849 417
Fragments arranged by size:
1290 849 417
PasI CC'CwG_GG
Cuts at: 0 49 126 633 1059 2036 2500 2556
Size: 49 77 507 426 977 464 56
Fragments arranged by size:
977 507 464 426 77 56 49
PciI A'CATG_T
Cuts at: 0 1286 2556
Size: 1286 1270
PflMI CCAn_nnn'nTGG
Cuts at: 0 887 1410 2556
Size: 887 523 1146
Fragments arranged by size:
1146 887 523
PfoI T'CCnGG_A
Cuts at: 0 1277 2556
Size: 1277 1279
PmlI CAC'GTG
Cuts at: 0 1308 2556
Size: 1308 1248
PpuMI rG'GwC_Cy
Cuts at: 0 1254 2556
Size: 1254 1302
PspOMI G'GGCC_C
Cuts at: 0 6 59 427 1810 2032 2496 2556
Size: 6 53 368 1383 222 464 60
Fragments arranged by size:
1383 464 368 222 60 53 6
PstI C_TGCA'G
Cuts at: 0 605 1136 1961 2556
Size: 605 531 825 595
Fragments arranged by size:
825 605 595 531
PvuII CAG'CTG
Cuts at: 0 579 990 1186 2556
Size: 579 411 196 1370
Fragments arranged by size:
1370 579 411 196
SacI G_AGCT'C
Cuts at: 0 446 2556
Size: 446 2110
SacII CC_GC'GG
Cuts at: 0 593 692 2556
Size: 593 99 1864
Fragments arranged by size:
1864 593 99
SbfI CC_TGCA'GG
Cuts at: 0 1961 2556
Size: 1961 595
SexAI A'CCwGG_T
Cuts at: 0 187 782 920 2089 2096 2556
Size: 187 595 138 1169 7 460
Fragments arranged by size:
1169 595 460 187 138 7
SfcI C'TryA_G
Cuts at: 0 601 855 1132 1957 2556
Size: 601 254 277 825 599
Fragments arranged by size:
825 601 599 277 254
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 662 901 2556
Size: 662 239 1655
Fragments arranged by size:
1655 662 239
SfoI GGC'GCC
Cuts at: 0 2388 2556
Size: 2388 168
SmaI CCC'GGG
Cuts at: 0 273 529 2556
Size: 273 256 2027
Fragments arranged by size:
2027 273 256
SrfI GCCC'GGGC
Cuts at: 0 273 2556
Size: 273 2283
StuI AGG'CCT
Cuts at: 0 904 1801 2556
Size: 904 897 755
Fragments arranged by size:
904 897 755
StyI C'CwwG_G
Cuts at: 0 353 449 881 2511 2556
Size: 353 96 432 1630 45
Fragments arranged by size:
1630 432 353 96 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 612 879 1365 2556
Size: 612 267 486 1191
Fragments arranged by size:
1191 612 486 267
TatI w'GTAC_w
Cuts at: 0 1289 2556
Size: 1289 1267
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1646 1678 2556
Size: 1646 32 878
Fragments arranged by size:
1646 878 32
Tth111I GACn'n_nGTC
Cuts at: 0 922 2556
Size: 922 1634
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 888 2151 2214 2355 2556
Size: 888 1263 63 141 201
Fragments arranged by size:
1263 888 201 141 63
XmaI C'CCGG_G
Cuts at: 0 271 527 2556
Size: 271 256 2029
Fragments arranged by size:
2029 271 256
ZraI GAC'GTC
Cuts at: 0 774 2556
Size: 774 1782
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AlfI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SacII SbfI SexAI SfcI SfiI
SfoI SmaI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI AleI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI
BplI BpuEI BsaBI BsaWI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BssHII BstBI BstZ17I ClaI CspCI DraI
DrdI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI MfeI MluI NdeI NotI NruI NsiI
PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI SapI ScaI SgfI SgrAI SmlI
SnaBI SpeI SphI SspI SwaI XbaI XhoI XmnI