Restriction map for
pF1KE0075
(Linear) (Six-base) MAPSORT of: KIEE0075.seq Check: 702 from: 1 to: 4341
KIEE0075.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AatII G_ACGT'C
Cuts at: 0 1310 4341
Size: 1310 3031
AccI GT'mk_AC
Cuts at: 0 569 4341
Size: 569 3772
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1548 1642 2526 2539 2548 4001 4341
Size: 1548 94 884 13 9 1453 340
Fragments arranged by size:
1548 1453 884 340 94 13 9
AfeI AGC'GCT
Cuts at: 0 521 2751 4341
Size: 521 2230 1590
Fragments arranged by size:
2230 1590 521
AflIII A'CryG_T
Cuts at: 0 572 2018 2405 3362 4341
Size: 572 1446 387 957 979
Fragments arranged by size:
1446 979 957 572 387
AhdI GACnn_n'nnGTC
Cuts at: 0 3870 4341
Size: 3870 471
AleI CACnn'nnGTG
Cuts at: 0 2017 2308 4108 4341
Size: 2017 291 1800 233
Fragments arranged by size:
2017 1800 291 233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3361 3383 4096 4341
Size: 3361 22 713 245
Fragments arranged by size:
3361 713 245 22
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4042 4074 4341
Size: 4042 32 267
Fragments arranged by size:
4042 267 32
AlwNI CAG_nnn'CTG
Cuts at: 0 56 164 1444 2549 2585 2718 4341
Size: 56 108 1280 1105 36 133 1623
Fragments arranged by size:
1623 1280 1105 133 108 56 36
ApaI G_GGCC'C
Cuts at: 0 4011 4021 4032 4341
Size: 4011 10 11 309
Fragments arranged by size:
4011 309 11 10
ApaLI G'TGCA_C
Cuts at: 0 4141 4341
Size: 4141 200
ApoI r'AATT_y
Cuts at: 0 787 933 1475 3971 4341
Size: 787 146 542 2496 370
Fragments arranged by size:
2496 787 542 370 146
AseI AT'TA_AT
Cuts at: 0 2741 4341
Size: 2741 1600
AvaI C'yCGr_G
Cuts at: 0 69 361 2167 2306 2345 3843 4178 4322
Size: 69 292 1806 139 39 1498 335 144
Cuts at: 4322 4341
Size: 19
Fragments arranged by size:
1806 1498 335 292 144 139 69 39
19
AvrII C'CTAG_G
Cuts at: 0 744 4341
Size: 744 3597
BanI G'GyrC_C
Cuts at: 0 64 1399 2299 3385 4341
Size: 64 1335 900 1086 956
Fragments arranged by size:
1335 1086 956 900 64
BanII G_rGCy'C
Cuts at: 0 29 2104 2174 2903 4011 4021 4032 4341
Size: 29 2075 70 729 1108 10 11 309
Fragments arranged by size:
2075 1108 729 309 70 29 11 10
BbeI G_GCGC'C
Cuts at: 0 3389 4341
Size: 3389 952
BbsI GAAGACnn'nnnn_
Cuts at: 0 3126 3780 4341
Size: 3126 654 561
Fragments arranged by size:
3126 654 561
BbvCI CC'TCA_GC
Cuts at: 0 854 2089 3700 4341
Size: 854 1235 1611 641
Fragments arranged by size:
1611 1235 854 641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3102 3136 4341
Size: 3102 34 1205
Fragments arranged by size:
3102 1205 34
BfrBI ATG'CAT
Cuts at: 0 2572 4341
Size: 2572 1769
BglI GCCn_nnn'nGGC
Cuts at: 0 231 1642 2297 2812 4341
Size: 231 1411 655 515 1529
Fragments arranged by size:
1529 1411 655 515 231
BglII A'GATC_T
Cuts at: 0 2051 4341
Size: 2051 2290
BlpI GC'TnA_GC
Cuts at: 0 2036 4341
Size: 2036 2305
Bme1580I G_kGCm'C
Cuts at: 0 69 3245 4011 4021 4032 4085 4145 4341
Size: 69 3176 766 10 11 53 60 196
Fragments arranged by size:
3176 766 196 69 60 53 11 10
BmgBI CAC'GTC
Cuts at: 0 1652 4251 4302 4341
Size: 1652 2599 51 39
Fragments arranged by size:
2599 1652 51 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 277 1462 2133 2595 2910 4341
Size: 277 1185 671 462 315 1431
Fragments arranged by size:
1431 1185 671 462 315 277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1161 2061 4204 4341
Size: 1161 900 2143 137
Fragments arranged by size:
2143 1161 900 137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 205 532 1207 1708 2136 3376 3539
Size: 104 101 327 675 501 428 1240 163
Cuts at: 3539 4341
Size: 802
Fragments arranged by size:
1240 802 675 501 428 327 163 104
101
Bpu10I CC'TnA_GC
Cuts at: 0 854 2089 3593 3700 4341
Size: 854 1235 1504 107 641
Fragments arranged by size:
1504 1235 854 641 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 358 4341
Size: 358 3983
BsaAI yAC'GTr
Cuts at: 0 2019 4239 4341
Size: 2019 2220 102
Fragments arranged by size:
2220 2019 102
BsaBI GATnn'nnATC
Cuts at: 0 1799 4341
Size: 1799 2542
BsaHI Gr'CG_yC
Cuts at: 0 170 1307 1418 3386 4341
Size: 170 1137 111 1968 955
Fragments arranged by size:
1968 1137 955 170 111
BsaWI w'CCGG_w
Cuts at: 0 4253 4341
Size: 4253 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 367 389 397 419 1625 1655 2119 2149
Size: 367 22 8 22 1206 30 464 30
Cuts at: 2149 2463 2493 3349 3379 4341
Size: 314 30 856 30 962
Fragments arranged by size:
1206 962 856 464 367 314 30 30
30 30 22 22 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 307 391 1030 3721 4278 4327 4341
Size: 307 84 639 2691 557 49 14
Fragments arranged by size:
2691 639 557 307 84 49 14
BseYI C'CCAG_C
Cuts at: 0 157 830 1376 2280 2746 3402 3604 3728
Size: 157 673 546 904 466 656 202 124
Cuts at: 3728 4013 4053 4341
Size: 285 40 288
Fragments arranged by size:
904 673 656 546 466 288 285 202
157 124 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 2080 2360 2518 3096 4341
Size: 133 1947 280 158 578 1245
Fragments arranged by size:
1947 1245 578 280 158 133
BsiEI CG_ry'CG
Cuts at: 0 79 899 3158 3194 4341
Size: 79 820 2259 36 1147
Fragments arranged by size:
2259 1147 820 79 36
BsiHKAI G_wGCw'C
Cuts at: 0 1124 1465 2113 3362 4145 4341
Size: 1124 341 648 1249 783 196
Fragments arranged by size:
1249 1124 783 648 341 196
BsmI GAATG_Cn'
Cuts at: 0 3987 4312 4341
Size: 3987 325 29
Fragments arranged by size:
3987 325 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2309 3953 4341
Size: 2309 1644 388
Fragments arranged by size:
2309 1644 388
Bsp1286I G_dGCh'C
Cuts at: 0 29 69 1124 1465 2104 2113 2174 2903
Size: 29 40 1055 341 639 9 61 729
Cuts at: 2903 3245 3362 4011 4021 4032 4085 4145 4341
Size: 342 117 649 10 11 53 60 196
Fragments arranged by size:
1055 729 649 639 342 341 196 117
61 60 53 40 29 11 10 9
BspHI T'CATG_A
Cuts at: 0 470 4341
Size: 470 3871
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2138 4341
Size: 2138 2203
BsrBI CCG'CTC
Cuts at: 0 1705 4341
Size: 1705 2636
BsrDI GCAATG_nn'
Cuts at: 0 1668 4341
Size: 1668 2673
BsrFI r'CCGG_y
Cuts at: 0 78 152 2668 4341
Size: 78 74 2516 1673
Fragments arranged by size:
2516 1673 78 74
BsrGI T'GTAC_A
Cuts at: 0 575 4341
Size: 575 3766
BssSI C'ACGA_G
Cuts at: 0 3121 4341
Size: 3121 1220
BstBI TT'CG_AA
Cuts at: 0 4273 4341
Size: 4273 68
BstXI CCAn_nnnn'nTGG
Cuts at: 0 439 1278 2253 3402 4068 4341
Size: 439 839 975 1149 666 273
Fragments arranged by size:
1149 975 839 666 439 273
BstYI r'GATC_y
Cuts at: 0 2051 3424 4341
Size: 2051 1373 917
Fragments arranged by size:
2051 1373 917
BstZ17I GTA'TAC
Cuts at: 0 570 4341
Size: 570 3771
Bsu36I CC'TnA_GG
Cuts at: 0 1880 3545 4341
Size: 1880 1665 796
Fragments arranged by size:
1880 1665 796
BtgI C'CryG_G
Cuts at: 0 36 1403 1757 3245 4067 4215 4341
Size: 36 1367 354 1488 822 148 126
Fragments arranged by size:
1488 1367 822 354 148 126 36
BtsI GCAGTG_nn'
Cuts at: 0 592 2160 4061 4341
Size: 592 1568 1901 280
Fragments arranged by size:
1901 1568 592 280
DraI TTT'AAA
Cuts at: 0 264 4341
Size: 264 4077
DraIII CAC_nnn'GTG
Cuts at: 0 3759 4341
Size: 3759 582
DrdI GACnn_nn'nnGTC
Cuts at: 0 407 1312 2638 3442 3690 4341
Size: 407 905 1326 804 248 651
Fragments arranged by size:
1326 905 804 651 407 248
EaeI y'GGCC_r
Cuts at: 0 76 213 896 1325 1682 2824 3155 3317
Size: 76 137 683 429 357 1142 331 162
Cuts at: 3317 3633 3934 4341
Size: 316 301 407
Fragments arranged by size:
1142 683 429 407 357 331 316 301
162 137 76
EagI C'GGCC_G
Cuts at: 0 76 896 3155 4341
Size: 76 820 2259 1186
Fragments arranged by size:
2259 1186 820 76
EarI CTCTTCn'nnn_
Cuts at: 0 440 791 3052 4341
Size: 440 351 2261 1289
Fragments arranged by size:
2261 1289 440 351
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3061 4185 4341
Size: 3061 1124 156
Fragments arranged by size:
3061 1124 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 205 532 1207 1548 1642 1708 2136
Size: 104 101 327 675 341 94 66 428
Cuts at: 2136 2526 2539 2548 3376 3539 4001 4341
Size: 390 13 9 828 163 462 340
Fragments arranged by size:
828 675 462 428 390 341 340 327
163 104 101 94 66 13 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1197 3703 4225 4341
Size: 1197 2506 522 116
Fragments arranged by size:
2506 1197 522 116
EcoO109I rG'GnC_Cy
Cuts at: 0 329 2127 3745 3872 3912 4007 4017 4018
Size: 329 1798 1618 127 40 95 10 1
Cuts at: 4018 4028 4230 4319 4341
Size: 10 202 89 22
Fragments arranged by size:
1798 1618 329 202 127 95 89 40
22 10 10 1
EcoRI G'AATT_C
Cuts at: 0 787 4341
Size: 787 3554
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1610 4341
Size: 1610 2731
HaeII r_GCGC'y
Cuts at: 0 226 523 1070 2753 3184 3389 4341
Size: 226 297 547 1683 431 205 952
Fragments arranged by size:
1683 952 547 431 297 226 205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 611 643 2119 2151 3856 3888 4341
Size: 611 32 1476 32 1705 32 453
Fragments arranged by size:
1705 1476 611 453 32 32 32
HincII GTy'rAC
Cuts at: 0 2705 4341
Size: 2705 1636
KasI G'GCGC_C
Cuts at: 0 3385 4341
Size: 3385 956
MfeI C'AATT_G
Cuts at: 0 2969 4341
Size: 2969 1372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 2171 2480 3686 4341
Size: 375 1796 309 1206 655
Fragments arranged by size:
1796 1206 655 375 309
MscI TGG'CCA
Cuts at: 0 1327 3936 4341
Size: 1327 2609 405
Fragments arranged by size:
2609 1327 405
MslI CAynn'nnrTG
Cuts at: 0 1226 1321 2017 2308 3097 3169 3277 4066
Size: 1226 95 696 291 789 72 108 789
Cuts at: 4066 4108 4341
Size: 42 233
Fragments arranged by size:
1226 789 789 696 291 233 108 95
72 42
MspA1I CmG'CkG
Cuts at: 0 38 53 228 830 1331 1732 2035 2093
Size: 38 15 175 602 501 401 303 58
Cuts at: 2093 3647 4013 4341
Size: 1554 366 328
Fragments arranged by size:
1554 602 501 401 366 328 303 175
58 38 15
NaeI GCC'GGC
Cuts at: 0 80 4341
Size: 80 4261
NarI GG'CG_CC
Cuts at: 0 3386 4341
Size: 3386 955
NcoI C'CATG_G
Cuts at: 0 1757 4067 4341
Size: 1757 2310 274
Fragments arranged by size:
2310 1757 274
NdeI CA'TA_TG
Cuts at: 0 1206 4341
Size: 1206 3135
NgoMIV G'CCGG_C
Cuts at: 0 78 4341
Size: 78 4263
NsiI A_TGCA'T
Cuts at: 0 2574 4341
Size: 2574 1767
NspI r_CATG'y
Cuts at: 0 576 2409 2868 3366 4341
Size: 576 1833 459 498 975
Fragments arranged by size:
1833 975 576 498 459
PasI CC'CwG_GG
Cuts at: 0 1395 4159 4341
Size: 1395 2764 182
Fragments arranged by size:
2764 1395 182
PciI A'CATG_T
Cuts at: 0 572 2405 3362 4341
Size: 572 1833 957 979
Fragments arranged by size:
1833 979 957 572
PflMI CCAn_nnn'nTGG
Cuts at: 0 2528 4341
Size: 2528 1813
PfoI T'CCnGG_A
Cuts at: 0 791 1184 3377 3882 4341
Size: 791 393 2193 505 459
Fragments arranged by size:
2193 791 505 459 393
PmlI CAC'GTG
Cuts at: 0 2019 4239 4341
Size: 2019 2220 102
Fragments arranged by size:
2220 2019 102
PpuMI rG'GwC_Cy
Cuts at: 0 2127 3872 3912 4230 4341
Size: 2127 1745 40 318 111
Fragments arranged by size:
2127 1745 318 111 40
PspOMI G'GGCC_C
Cuts at: 0 4007 4017 4028 4341
Size: 4007 10 11 313
Fragments arranged by size:
4007 313 11 10
PspXI vC'TCGA_Gb
Cuts at: 0 2167 4341
Size: 2167 2174
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2902 2934 4341
Size: 2902 32 1407
Fragments arranged by size:
2902 1407 32
PstI C_TGCA'G
Cuts at: 0 1082 1449 2995 4298 4341
Size: 1082 367 1546 1303 43
Fragments arranged by size:
1546 1303 1082 367 43
PvuII CAG'CTG
Cuts at: 0 830 2093 4341
Size: 830 1263 2248
Fragments arranged by size:
2248 1263 830
RsrII CG'GwC_CG
Cuts at: 0 135 4341
Size: 135 4206
SacII CC_GC'GG
Cuts at: 0 39 4341
Size: 39 4302
SanDI GG'GwC_CC
Cuts at: 0 3872 3912 4341
Size: 3872 40 429
Fragments arranged by size:
3872 429 40
SfcI C'TryA_G
Cuts at: 0 1078 1445 1914 1932 2991 3945 4294 4341
Size: 1078 367 469 18 1059 954 349 47
Fragments arranged by size:
1078 1059 954 469 367 349 47 18
SfoI GGC'GCC
Cuts at: 0 3387 4341
Size: 3387 954
SmaI CCC'GGG
Cuts at: 0 71 4324 4341
Size: 71 4253 17
Fragments arranged by size:
4253 71 17
SmlI C'TyrA_G
Cuts at: 0 361 2167 4341
Size: 361 1806 2174
Fragments arranged by size:
2174 1806 361
StuI AGG'CCT
Cuts at: 0 1192 1885 3698 4341
Size: 1192 693 1813 643
Fragments arranged by size:
1813 1192 693 643
StyI C'CwwG_G
Cuts at: 0 197 744 1757 2246 4067 4341
Size: 197 547 1013 489 1821 274
Fragments arranged by size:
1821 1013 547 489 274 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 375 1018 2434 3845 4341
Size: 375 643 1416 1411 496
Fragments arranged by size:
1416 1411 643 496 375
TatI w'GTAC_w
Cuts at: 0 575 2021 3059 4341
Size: 575 1446 1038 1282
Fragments arranged by size:
1446 1282 1038 575
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 589 621 1625 1657 4341
Size: 367 32 190 32 1004 32 2684
Fragments arranged by size:
2684 1004 367 190 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2791 3690 4341
Size: 2791 899 651
Fragments arranged by size:
2791 899 651
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1275 4341
Size: 1275 3066
XhoI C'TCGA_G
Cuts at: 0 361 2167 4341
Size: 361 1806 2174
Fragments arranged by size:
2174 1806 361
XmaI C'CCGG_G
Cuts at: 0 69 4322 4341
Size: 69 4253 19
Fragments arranged by size:
4253 69 19
XmnI GAAnn'nnTTC
Cuts at: 0 1297 4341
Size: 1297 3044
ZraI GAC'GTC
Cuts at: 0 1308 4341
Size: 1308 3033
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII
BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII KasI MfeI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmlI PpuMI PspOMI PspXI
PsrI PstI PvuII RsrII SacII SanDI SfcI SfoI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AscI AsiSI BaeI
BamHI BciVI BclI BmtI BpuEI BsiWI BspEI BssHII
BstAPI BstEII BtgZI ClaI CspCI EcoICRI EcoRV FseI
FspI FspAI HindIII HpaI KpnI MluI NheI NotI
NruI PacI PmeI PpiI PshAI PsiI PvuI SacI
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI XbaI