Restriction map for
pF1KE0073
(Linear) (Six-base) MAPSORT of: KIEE0073.seq Check: 7374 from: 1 to: 3960
KIEE0073.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 0 683 3960
Size: 683 3277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3451 3550 3960
Size: 3451 99 410
Fragments arranged by size:
3451 410 99
AfeI AGC'GCT
Cuts at: 0 2169 3672 3960
Size: 2169 1503 288
Fragments arranged by size:
2169 1503 288
AflIII A'CryG_T
Cuts at: 0 734 3960
Size: 734 3226
AhdI GACnn_n'nnGTC
Cuts at: 0 1557 3174 3960
Size: 1557 1617 786
Fragments arranged by size:
1617 1557 786
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3689 3847 3960
Size: 3689 158 113
Fragments arranged by size:
3689 158 113
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2440 2472 3960
Size: 2440 32 1488
Fragments arranged by size:
2440 1488 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1323 1563 1707 3522 3605 3840 3960
Size: 1323 240 144 1815 83 235 120
Fragments arranged by size:
1815 1323 240 235 144 120 83
ApaI G_GGCC'C
Cuts at: 0 131 2035 2523 2661 3370 3960
Size: 131 1904 488 138 709 590
Fragments arranged by size:
1904 709 590 488 138 131
ApaLI G'TGCA_C
Cuts at: 0 262 3960
Size: 262 3698
ApoI r'AATT_y
Cuts at: 0 1113 3960
Size: 1113 2847
AvaI C'yCGr_G
Cuts at: 0 303 367 705 720 810 1093 1741 1947
Size: 303 64 338 15 90 283 648 206
Cuts at: 1947 2013 2143 2218 2515 2619 2709 3369 3881
Size: 66 130 75 297 104 90 660 512
Cuts at: 3881 3960
Size: 79
Fragments arranged by size:
660 648 512 338 303 297 283 206
130 104 90 90 79 75 66 64
15
BamHI G'GATC_C
Cuts at: 0 144 3960
Size: 144 3816
BanI G'GyrC_C
Cuts at: 0 538 714 814 1001 1308 1831 1846 1863
Size: 538 176 100 187 307 523 15 17
Cuts at: 1863 1989 2347 2743 2866 2988 3268 3291 3427
Size: 126 358 396 123 122 280 23 136
Cuts at: 3427 3735 3960
Size: 308 225
Fragments arranged by size:
538 523 396 358 308 307 280 225
187 176 136 126 123 122 100 23
17 15
BanII G_rGCy'C
Cuts at: 0 26 131 200 589 986 997 2035 2162
Size: 26 105 69 389 397 11 1038 127
Cuts at: 2162 2425 2523 2626 2661 2863 2926 3370 3960
Size: 263 98 103 35 202 63 444 590
Fragments arranged by size:
1038 590 444 397 389 263 202 127
105 103 98 69 63 35 26 11
BbeI G_GCGC'C
Cuts at: 0 718 818 1312 1835 1867 1993 3272 3295
Size: 718 100 494 523 32 126 1279 23
Cuts at: 3295 3431 3739 3960
Size: 136 308 221
Fragments arranged by size:
1279 718 523 494 308 221 136 126
100 32 23
BbvCI CC'TCA_GC
Cuts at: 0 1043 3807 3960
Size: 1043 2764 153
Fragments arranged by size:
2764 1043 153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 373 407 2037 2071 3775 3809 3960
Size: 373 34 1630 34 1704 34 151
Fragments arranged by size:
1704 1630 373 151 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1235 3960
Size: 1235 2725
BglI GCCn_nnn'nGGC
Cuts at: 0 401 559 1095 1484 1760 1784 2231 3380
Size: 401 158 536 389 276 24 447 1149
Cuts at: 3380 3525 3562 3571 3900 3960
Size: 145 37 9 329 60
Fragments arranged by size:
1149 536 447 401 389 329 276 158
145 60 37 24 9
BlpI GC'TnA_GC
Cuts at: 0 1727 3960
Size: 1727 2233
Bme1580I G_kGCm'C
Cuts at: 0 131 266 1123 1162 1508 1849 2035 2078
Size: 131 135 857 39 346 341 186 43
Cuts at: 2078 2523 2661 2991 3370 3385 3896 3960
Size: 445 138 330 379 15 511 64
Fragments arranged by size:
857 511 445 379 346 341 330 186
138 135 131 64 43 39 15
BmgBI CAC'GTC
Cuts at: 0 1749 2780 3017 3864 3960
Size: 1749 1031 237 847 96
Fragments arranged by size:
1749 1031 847 237 96
BmrI ACTGGGnnnn_n'
Cuts at: 0 131 3960
Size: 131 3829
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 735 3150 3960
Size: 735 2415 810
Fragments arranged by size:
2415 810 735
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 609 670 981 1000 1261 2711 3415
Size: 528 81 61 311 19 261 1450 704
Cuts at: 3415 3478 3960
Size: 63 482
Fragments arranged by size:
1450 704 528 482 311 261 81 63
61 19
Bpu10I CC'TnA_GC
Cuts at: 0 1043 3807 3960
Size: 1043 2764 153
Fragments arranged by size:
2764 1043 153
BsaI GGTCTCn'nnnn_
Cuts at: 0 67 1913 2584 2808 3960
Size: 67 1846 671 224 1152
Fragments arranged by size:
1846 1152 671 224 67
BsaAI yAC'GTr
Cuts at: 0 2073 2856 3960
Size: 2073 783 1104
Fragments arranged by size:
2073 1104 783
BsaHI Gr'CG_yC
Cuts at: 0 715 815 1309 1684 1832 1839 1864 1990
Size: 715 100 494 375 148 7 25 126
Cuts at: 1990 2995 3269 3292 3428 3736 3872 3960
Size: 1005 274 23 136 308 136 88
Fragments arranged by size:
1005 715 494 375 308 274 148 136
136 126 100 88 25 23 7
BsaWI w'CCGG_w
Cuts at: 0 76 1247 2895 3960
Size: 76 1171 1648 1065
Fragments arranged by size:
1648 1171 1065 76
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1286 1988 3960
Size: 1286 702 1972
Fragments arranged by size:
1972 1286 702
BseYI C'CCAG_C
Cuts at: 0 162 869 890 908 1022 1142 1165 1201
Size: 162 707 21 18 114 120 23 36
Cuts at: 1201 1796 1981 2164 2299 2377 2418 2437 3594
Size: 595 185 183 135 78 41 19 1157
Cuts at: 3594 3815 3960
Size: 221 145
Fragments arranged by size:
1157 707 595 221 185 183 162 145
135 120 114 78 41 36 23 21
19 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 2267 3837 3960
Size: 367 1900 1570 123
Fragments arranged by size:
1900 1570 367 123
BsiEI CG_ry'CG
Cuts at: 0 508 2256 2820 3960
Size: 508 1748 564 1140
Fragments arranged by size:
1748 1140 564 508
BsiHKAI G_wGCw'C
Cuts at: 0 266 464 3151 3637 3668 3960
Size: 266 198 2687 486 31 292
Fragments arranged by size:
2687 486 292 266 198 31
BsmI GAATG_Cn'
Cuts at: 0 3912 3960
Size: 3912 48
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2971 3960
Size: 2971 989
Bsp1286I G_dGCh'C
Cuts at: 0 26 131 200 266 464 589 986 997
Size: 26 105 69 66 198 125 397 11
Cuts at: 997 1123 1162 1508 1849 2035 2078 2162 2425
Size: 126 39 346 341 186 43 84 263
Cuts at: 2425 2523 2626 2661 2863 2926 2991 3151 3370
Size: 98 103 35 202 63 65 160 219
Cuts at: 3370 3385 3637 3668 3896 3960
Size: 15 252 31 228 64
Fragments arranged by size:
397 346 341 263 252 228 219 202
198 186 160 126 125 105 103 98
84 69 66 65 64 63 43 39
35 31 26 15 11
BspEI T'CCGG_A
Cuts at: 0 2895 3960
Size: 2895 1065
BspHI T'CATG_A
Cuts at: 0 1667 3960
Size: 1667 2293
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1931 3390 3960
Size: 1931 1459 570
Fragments arranged by size:
1931 1459 570
BsrFI r'CCGG_y
Cuts at: 0 558 1235 2183 2251 2255 2611 2799 3235
Size: 558 677 948 68 4 356 188 436
Cuts at: 3235 3960
Size: 725
Fragments arranged by size:
948 725 677 558 436 356 188 68
4
BssHII G'CGCG_C
Cuts at: 0 158 214 2408 3960
Size: 158 56 2194 1552
Fragments arranged by size:
2194 1552 158 56
BssSI C'ACGA_G
Cuts at: 0 375 3150 3960
Size: 375 2775 810
Fragments arranged by size:
2775 810 375
BstAPI GCAn_nnn'nTGC
Cuts at: 0 261 1707 3605 3960
Size: 261 1446 1898 355
Fragments arranged by size:
1898 1446 355 261
BstEII G'GTnAC_C
Cuts at: 0 3231 3960
Size: 3231 729
BstXI CCAn_nnnn'nTGG
Cuts at: 0 796 2403 3645 3960
Size: 796 1607 1242 315
Fragments arranged by size:
1607 1242 796 315
BstYI r'GATC_y
Cuts at: 0 144 3446 3461 3476 3960
Size: 144 3302 15 15 484
Fragments arranged by size:
3302 484 144 15 15
Bsu36I CC'TnA_GG
Cuts at: 0 3567 3960
Size: 3567 393
BtgI C'CryG_G
Cuts at: 0 294 609 655 3258 3960
Size: 294 315 46 2603 702
Fragments arranged by size:
2603 702 315 294 46
BtsI GCAGTG_nn'
Cuts at: 0 2612 3841 3849 3960
Size: 2612 1229 8 111
Fragments arranged by size:
2612 1229 111 8
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 336 371 3960
Size: 336 35 3589
Fragments arranged by size:
3589 336 35
DraIII CAC_nnn'GTG
Cuts at: 0 555 3960
Size: 555 3405
EaeI y'GGCC_r
Cuts at: 0 48 219 279 297 821 1358 2253 2393
Size: 48 171 60 18 524 537 895 140
Cuts at: 2393 2460 2609 2817 3960
Size: 67 149 208 1143
Fragments arranged by size:
1143 895 537 524 208 171 149 140
67 60 48 18
EagI C'GGCC_G
Cuts at: 0 2253 2817 3960
Size: 2253 564 1143
Fragments arranged by size:
2253 1143 564
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 484 1455 1898 1991 3960
Size: 484 971 443 93 1969
Fragments arranged by size:
1969 971 484 443 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 609 670 981 1000 1261 2711 3415
Size: 528 81 61 311 19 261 1450 704
Cuts at: 3415 3451 3478 3550 3960
Size: 36 27 72 410
Fragments arranged by size:
1450 704 528 410 311 261 81 72
61 36 27 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3397 3960
Size: 3397 563
EcoO109I rG'GnC_Cy
Cuts at: 0 105 127 128 371 481 1029 1189 2031
Size: 105 22 1 243 110 548 160 842
Cuts at: 2031 2032 2519 2520 2657 2658 2665 3077 3366
Size: 1 487 1 137 1 7 412 289
Cuts at: 3366 3563 3699 3960
Size: 197 136 261
Fragments arranged by size:
842 548 487 412 289 261 243 197
160 137 136 110 105 22 7 1
1 1 1
HaeII r_GCGC'y
Cuts at: 0 718 806 818 1312 1442 1653 1835 1867
Size: 718 88 12 494 130 211 182 32
Cuts at: 1867 1993 2171 2184 3013 3272 3295 3431 3629
Size: 126 178 13 829 259 23 136 198
Cuts at: 3629 3674 3739 3960
Size: 45 65 221
Fragments arranged by size:
829 718 494 259 221 211 198 182
178 136 130 126 88 65 45 32
23 13 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1543 1575 2869 2901 3160 3163 3192 3195
Size: 1543 32 1294 32 259 3 29 3
Cuts at: 3195 3207 3239 3634 3666 3960
Size: 12 32 395 32 294
Fragments arranged by size:
1543 1294 395 294 259 32 32 32
32 29 12 3 3
HincII GTy'rAC
Cuts at: 0 33 684 3960
Size: 33 651 3276
Fragments arranged by size:
3276 651 33
KasI G'GCGC_C
Cuts at: 0 714 814 1308 1831 1863 1989 3268 3291
Size: 714 100 494 523 32 126 1279 23
Cuts at: 3291 3427 3735 3960
Size: 136 308 225
Fragments arranged by size:
1279 714 523 494 308 225 136 126
100 32 23
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1131 3080 3950 3960
Size: 1131 1949 870 10
Fragments arranged by size:
1949 1131 870 10
MscI TGG'CCA
Cuts at: 0 50 1360 3960
Size: 50 1310 2600
Fragments arranged by size:
2600 1310 50
MslI CAynn'nnrTG
Cuts at: 0 190 1354 3960
Size: 190 1164 2606
Fragments arranged by size:
2606 1164 190
MspA1I CmG'CkG
Cuts at: 0 576 629 765 781 1169 1320 1406 1704
Size: 576 53 136 16 388 151 86 298
Cuts at: 1704 1960 2133 2276 2696 2903 3425 3602 3691
Size: 256 173 143 420 207 522 177 89
Cuts at: 3691 3960
Size: 269
Fragments arranged by size:
576 522 420 388 298 269 256 207
177 173 151 143 136 89 86 53
16
NaeI GCC'GGC
Cuts at: 0 560 2185 2253 2257 2613 3960
Size: 560 1625 68 4 356 1347
Fragments arranged by size:
1625 1347 560 356 68 4
NarI GG'CG_CC
Cuts at: 0 715 815 1309 1832 1864 1990 3269 3292
Size: 715 100 494 523 32 126 1279 23
Cuts at: 3292 3428 3736 3960
Size: 136 308 224
Fragments arranged by size:
1279 715 523 494 308 224 136 126
100 32 23
NgoMIV G'CCGG_C
Cuts at: 0 558 2183 2251 2255 2611 3960
Size: 558 1625 68 4 356 1349
Fragments arranged by size:
1625 1349 558 356 68 4
NspI r_CATG'y
Cuts at: 0 738 1257 1287 1431 3687 3960
Size: 738 519 30 144 2256 273
Fragments arranged by size:
2256 738 519 273 144 30
PasI CC'CwG_GG
Cuts at: 0 2733 2926 3740 3960
Size: 2733 193 814 220
Fragments arranged by size:
2733 814 220 193
PciI A'CATG_T
Cuts at: 0 734 3960
Size: 734 3226
PflMI CCAn_nnn'nTGG
Cuts at: 0 648 1239 3456 3522 3960
Size: 648 591 2217 66 438
Fragments arranged by size:
2217 648 591 438 66
PfoI T'CCnGG_A
Cuts at: 0 518 2680 3960
Size: 518 2162 1280
Fragments arranged by size:
2162 1280 518
PmlI CAC'GTG
Cuts at: 0 2073 2856 3960
Size: 2073 783 1104
Fragments arranged by size:
2073 1104 783
PpuMI rG'GwC_Cy
Cuts at: 0 2665 3077 3960
Size: 2665 412 883
Fragments arranged by size:
2665 883 412
PshAI GACnn'nnGTC
Cuts at: 0 2849 3960
Size: 2849 1111
PspOMI G'GGCC_C
Cuts at: 0 127 2031 2519 2657 3366 3960
Size: 127 1904 488 138 709 594
Fragments arranged by size:
1904 709 594 488 138 127
PspXI vC'TCGA_Gb
Cuts at: 0 3881 3960
Size: 3881 79
PstI C_TGCA'G
Cuts at: 0 1181 1328 1538 2042 2102 2138 3960
Size: 1181 147 210 504 60 36 1822
Fragments arranged by size:
1822 1181 504 210 147 60 36
PvuII CAG'CTG
Cuts at: 0 765 2133 3602 3960
Size: 765 1368 1469 358
Fragments arranged by size:
1469 1368 765 358
RsrII CG'GwC_CG
Cuts at: 0 3283 3960
Size: 3283 677
SalI G'TCGA_C
Cuts at: 0 682 3960
Size: 682 3278
SanDI GG'GwC_CC
Cuts at: 0 3077 3960
Size: 3077 883
SbfI CC_TGCA'GG
Cuts at: 0 1181 3960
Size: 1181 2779
ScaI AGT'ACT
Cuts at: 0 2884 3960
Size: 2884 1076
SfcI C'TryA_G
Cuts at: 0 1177 1324 1534 2038 2098 2134 3484 3960
Size: 1177 147 210 504 60 36 1350 476
Fragments arranged by size:
1350 1177 504 476 210 147 60 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1760 3960
Size: 1760 2200
SfoI GGC'GCC
Cuts at: 0 716 816 1310 1833 1865 1991 3270 3293
Size: 716 100 494 523 32 126 1279 23
Cuts at: 3293 3429 3737 3960
Size: 136 308 223
Fragments arranged by size:
1279 716 523 494 308 223 136 126
100 32 23
SmaI CCC'GGG
Cuts at: 0 305 369 707 812 1095 1743 2145 2621
Size: 305 64 338 105 283 648 402 476
Cuts at: 2621 3371 3960
Size: 750 589
Fragments arranged by size:
750 648 589 476 402 338 305 283
105 64
SmlI C'TyrA_G
Cuts at: 0 3881 3960
Size: 3881 79
SpeI A'CTAG_T
Cuts at: 0 3204 3960
Size: 3204 756
SphI G_CATG'C
Cuts at: 0 1257 1287 1431 3960
Size: 1257 30 144 2529
Fragments arranged by size:
2529 1257 144 30
SrfI GCCC'GGGC
Cuts at: 0 3371 3960
Size: 3371 589
StuI AGG'CCT
Cuts at: 0 425 1478 2123 3805 3960
Size: 425 1053 645 1682 155
Fragments arranged by size:
1682 1053 645 425 155
StyI C'CwwG_G
Cuts at: 0 672 2444 3875 3960
Size: 672 1772 1431 85
Fragments arranged by size:
1772 1431 672 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 109 2608 3112 3300 3856 3960
Size: 109 2499 504 188 556 104
Fragments arranged by size:
2499 556 504 188 109 104
TatI w'GTAC_w
Cuts at: 0 2882 3960
Size: 2882 1078
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1249 1281 3960
Size: 1249 32 2679
Fragments arranged by size:
2679 1249 32
Tth111I GACn'n_nGTC
Cuts at: 0 2828 3960
Size: 2828 1132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1236 2766 3960
Size: 1236 1530 1194
Fragments arranged by size:
1530 1236 1194
XhoI C'TCGA_G
Cuts at: 0 3881 3960
Size: 3881 79
XmaI C'CCGG_G
Cuts at: 0 303 367 705 810 1093 1741 2143 2619
Size: 303 64 338 105 283 648 402 476
Cuts at: 2619 3369 3960
Size: 750 591
Fragments arranged by size:
750 648 591 476 402 338 303 283
105 64
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrFI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI EagI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PstI PvuII RsrII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AleI AscI
AseI AsiSI AvrII BaeI BbsI BclI BfrBI BglII
BmtI BpuEI BsaBI BsaXI BsiWI BsrBI BsrDI BsrGI
BstBI BstZ17I BtgZI ClaI DraI DrdI EarI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NcoI NdeI NheI NotI NruI
NsiI PacI PmeI PpiI PsiI PsrI PvuI SacI
SacII SapI SexAI SgfI SgrAI SnaBI SspI SwaI
XbaI XmnI ZraI