Restriction map for
pF1KE0072
(Linear) (Six-base) MAPSORT of: KIEE0072.seq Check: 8395 from: 1 to: 4020
KIEE0072.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3161 4020
Size: 3161 859
AccI GT'mk_AC
Cuts at: 0 867 4020
Size: 867 3153
Acc65I G'GTAC_C
Cuts at: 0 1734 3102 4020
Size: 1734 1368 918
Fragments arranged by size:
1734 1368 918
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 2367 3317 4020
Size: 878 1489 950 703
Fragments arranged by size:
1489 950 878 703
AflII C'TTAA_G
Cuts at: 0 2620 4020
Size: 2620 1400
AhdI GACnn_n'nnGTC
Cuts at: 0 1374 4020
Size: 1374 2646
AleI CACnn'nnGTG
Cuts at: 0 895 3010 4020
Size: 895 2115 1010
Fragments arranged by size:
2115 1010 895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3680 3890 4020
Size: 3680 210 130
Fragments arranged by size:
3680 210 130
AlwNI CAG_nnn'CTG
Cuts at: 0 583 922 1417 4020
Size: 583 339 495 2603
Fragments arranged by size:
2603 583 495 339
ApoI r'AATT_y
Cuts at: 0 194 1304 1630 1822 2646 2942 2964 4020
Size: 194 1110 326 192 824 296 22 1056
Fragments arranged by size:
1110 1056 824 326 296 194 192 22
AseI AT'TA_AT
Cuts at: 0 2441 4020
Size: 2441 1579
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 725 758 3094 3127 3610 3643 4020
Size: 725 33 2336 33 483 33 377
Fragments arranged by size:
2336 725 483 377 33 33 33
BanI G'GyrC_C
Cuts at: 0 160 1734 2554 2610 3102 4020
Size: 160 1574 820 56 492 918
Fragments arranged by size:
1574 918 820 492 160 56
BbeI G_GCGC'C
Cuts at: 0 164 4020
Size: 164 3856
BbvCI CC'TCA_GC
Cuts at: 0 2030 3699 4020
Size: 2030 1669 321
Fragments arranged by size:
2030 1669 321
BciVI GTATCCnnnnn_n'
Cuts at: 0 327 403 726 2514 4020
Size: 327 76 323 1788 1506
Fragments arranged by size:
1788 1506 327 323 76
BclI T'GATC_A
Cuts at: 0 1779 3855 4020
Size: 1779 2076 165
Fragments arranged by size:
2076 1779 165
BglII A'GATC_T
Cuts at: 0 344 2259 4020
Size: 344 1915 1761
Fragments arranged by size:
1915 1761 344
BmrI ACTGGGnnnn_n'
Cuts at: 0 829 1491 1792 3202 3911 4020
Size: 829 662 301 1410 709 109
Fragments arranged by size:
1410 829 709 662 301 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1548 4020
Size: 1548 2472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1369 1469 3560 4020
Size: 1369 100 2091 460
Fragments arranged by size:
2091 1369 460 100
Bpu10I CC'TnA_GC
Cuts at: 0 2030 3699 4020
Size: 2030 1669 321
Fragments arranged by size:
2030 1669 321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3027 3383 4020
Size: 3027 356 637
Fragments arranged by size:
3027 637 356
BsaI GGTCTCn'nnnn_
Cuts at: 0 3191 4020
Size: 3191 829
BsaHI Gr'CG_yC
Cuts at: 0 161 4020
Size: 161 3859
BsaWI w'CCGG_w
Cuts at: 0 301 4020
Size: 301 3719
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3013 3043 3942 3972 4020
Size: 3013 30 899 30 48
Fragments arranged by size:
3013 899 48 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 38 3274 3638 4020
Size: 38 3236 364 382
Fragments arranged by size:
3236 382 364 38
BseYI C'CCAG_C
Cuts at: 0 513 576 3131 3283 4020
Size: 513 63 2555 152 737
Fragments arranged by size:
2555 737 513 152 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3378 3650 3676 4020
Size: 3378 272 26 344
Fragments arranged by size:
3378 344 272 26
BsmI GAATG_Cn'
Cuts at: 0 1359 2223 2721 4020
Size: 1359 864 498 1299
Fragments arranged by size:
1359 1299 864 498
BspHI T'CATG_A
Cuts at: 0 1299 4020
Size: 1299 2721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1613 2744 3161 4020
Size: 1613 1131 417 859
Fragments arranged by size:
1613 1131 859 417
BsrDI GCAATG_nn'
Cuts at: 0 408 977 1118 1451 1758 4020
Size: 408 569 141 333 307 2262
Fragments arranged by size:
2262 569 408 333 307 141
BsrFI r'CCGG_y
Cuts at: 0 355 3441 4020
Size: 355 3086 579
Fragments arranged by size:
3086 579 355
BsrGI T'GTAC_A
Cuts at: 0 293 4020
Size: 293 3727
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1570 2548 3733 4020
Size: 1570 978 1185 287
Fragments arranged by size:
1570 1185 978 287
BstBI TT'CG_AA
Cuts at: 0 321 4020
Size: 321 3699
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2082 4020
Size: 2082 1938
BstYI r'GATC_y
Cuts at: 0 344 781 944 2259 2531 3054 4020
Size: 344 437 163 1315 272 523 966
Fragments arranged by size:
1315 966 523 437 344 272 163
BstZ17I GTA'TAC
Cuts at: 0 868 4020
Size: 868 3152
Bsu36I CC'TnA_GG
Cuts at: 0 3254 4020
Size: 3254 766
BtgI C'CryG_G
Cuts at: 0 3231 4020
Size: 3231 789
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3576 4020
Size: 3576 444
BtsI GCAGTG_nn'
Cuts at: 0 2585 3186 4020
Size: 2585 601 834
Fragments arranged by size:
2585 834 601
DraIII CAC_nnn'GTG
Cuts at: 0 2340 4020
Size: 2340 1680
EaeI y'GGCC_r
Cuts at: 0 3417 4020
Size: 3417 603
EarI CTCTTCn'nnn_
Cuts at: 0 42 56 323 719 1456 2386 2825 3371
Size: 42 14 267 396 737 930 439 546
Cuts at: 3371 3471 3759 4020
Size: 100 288 261
Fragments arranged by size:
930 737 546 439 396 288 267 261
100 42 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 1369 1469 2367 3317 3560 4020
Size: 878 491 100 898 950 243 460
Fragments arranged by size:
950 898 878 491 460 243 100
EcoNI CCTnn'n_nnAGG
Cuts at: 0 596 4020
Size: 596 3424
EcoO109I rG'GnC_Cy
Cuts at: 0 1612 4020
Size: 1612 2408
EcoRI G'AATT_C
Cuts at: 0 1630 2646 4020
Size: 1630 1016 1374
Fragments arranged by size:
1630 1374 1016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 966 4020
Size: 966 3054
FspI TGC'GCA
Cuts at: 0 2455 4020
Size: 2455 1565
HaeII r_GCGC'y
Cuts at: 0 164 362 4020
Size: 164 198 3658
Fragments arranged by size:
3658 198 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1160 1192 1360 1392 2330 2362 2491 2523
Size: 1160 32 168 32 938 32 129 32
Cuts at: 2523 3013 3045 3295 3307 3327 3339 4020
Size: 490 32 250 12 20 12 681
Fragments arranged by size:
1160 938 681 490 250 168 129 32
32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 0 2199 2784 4020
Size: 2199 585 1236
Fragments arranged by size:
2199 1236 585
KasI G'GCGC_C
Cuts at: 0 160 4020
Size: 160 3860
KpnI G_GTAC'C
Cuts at: 0 1738 3106 4020
Size: 1738 1368 914
Fragments arranged by size:
1738 1368 914
MfeI C'AATT_G
Cuts at: 0 2853 3033 4020
Size: 2853 180 987
Fragments arranged by size:
2853 987 180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 998 1995 4020
Size: 998 997 2025
Fragments arranged by size:
2025 998 997
MscI TGG'CCA
Cuts at: 0 3419 4020
Size: 3419 601
MslI CAynn'nnrTG
Cuts at: 0 895 1001 1246 1459 2080 2212 3010 3425
Size: 895 106 245 213 621 132 798 415
Cuts at: 3425 3917 4020
Size: 492 103
Fragments arranged by size:
895 798 621 492 415 245 213 132
106 103
MspA1I CmG'CkG
Cuts at: 0 580 2545 3135 4020
Size: 580 1965 590 885
Fragments arranged by size:
1965 885 590 580
NaeI GCC'GGC
Cuts at: 0 3443 4020
Size: 3443 577
NarI GG'CG_CC
Cuts at: 0 161 4020
Size: 161 3859
NcoI C'CATG_G
Cuts at: 0 3231 4020
Size: 3231 789
NgoMIV G'CCGG_C
Cuts at: 0 3441 4020
Size: 3441 579
NspI r_CATG'y
Cuts at: 0 1289 1659 2643 4020
Size: 1289 370 984 1377
Fragments arranged by size:
1377 1289 984 370
PflMI CCAn_nnn'nTGG
Cuts at: 0 1852 4020
Size: 1852 2168
PfoI T'CCnGG_A
Cuts at: 0 109 1346 4020
Size: 109 1237 2674
Fragments arranged by size:
2674 1237 109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2981 3013 4020
Size: 2981 32 1007
Fragments arranged by size:
2981 1007 32
PstI C_TGCA'G
Cuts at: 0 1413 1908 4020
Size: 1413 495 2112
Fragments arranged by size:
2112 1413 495
PvuII CAG'CTG
Cuts at: 0 2545 4020
Size: 2545 1475
SapI GCTCTTCn'nnn_
Cuts at: 0 1456 2825 3471 3759 4020
Size: 1456 1369 646 288 261
Fragments arranged by size:
1456 1369 646 288 261
ScaI AGT'ACT
Cuts at: 0 789 3265 4020
Size: 789 2476 755
Fragments arranged by size:
2476 789 755
SfcI C'TryA_G
Cuts at: 0 884 1409 1664 1904 2591 3060 3711 4020
Size: 884 525 255 240 687 469 651 309
Fragments arranged by size:
884 687 651 525 469 309 255 240
SfoI GGC'GCC
Cuts at: 0 162 4020
Size: 162 3858
SmlI C'TyrA_G
Cuts at: 0 2620 3042 3398 4020
Size: 2620 422 356 622
Fragments arranged by size:
2620 622 422 356
SpeI A'CTAG_T
Cuts at: 0 2515 4020
Size: 2515 1505
SphI G_CATG'C
Cuts at: 0 1659 4020
Size: 1659 2361
SspI AAT'ATT
Cuts at: 0 452 1215 1702 1980 4020
Size: 452 763 487 278 2040
Fragments arranged by size:
2040 763 487 452 278
StuI AGG'CCT
Cuts at: 0 3382 3481 3517 3697 4020
Size: 3382 99 36 180 323
Fragments arranged by size:
3382 323 180 99 36
StyI C'CwwG_G
Cuts at: 0 397 1846 2325 3231 3243 4020
Size: 397 1449 479 906 12 777
Fragments arranged by size:
1449 906 777 479 397 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 848 1510 3297 3813 4020
Size: 848 662 1787 516 207
Fragments arranged by size:
1787 848 662 516 207
TatI w'GTAC_w
Cuts at: 0 293 787 2849 3263 4020
Size: 293 494 2062 414 757
Fragments arranged by size:
2062 757 494 414 293
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 494 526 4020
Size: 494 32 3494
Fragments arranged by size:
3494 494 32
Tth111I GACn'n_nGTC
Cuts at: 0 2524 4020
Size: 2524 1496
XbaI T'CTAG_A
Cuts at: 0 2826 4020
Size: 2826 1194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1401 4020
Size: 1401 2619
XmnI GAAnn'nnTTC
Cuts at: 0 194 1214 1841 2147 3908 4020
Size: 194 1020 627 306 1761 112
Fragments arranged by size:
1761 1020 627 306 194 112
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AhdI AleI AlfI
AlwNI ApoI AseI BaeI BanI BbeI BbvCI BciVI
BclI BglII BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsmI BspHI
BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PflMI PfoI PpiI
PstI PvuII SapI ScaI SfcI SfoI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflIII AgeI AloI ApaI ApaLI
AscI AsiSI AvaI AvrII BamHI BanII BbsI BcgI
BfrBI BglI BlpI Bme1580I BmgBI BmtI BsaAI BsaBI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI BssHII
BssSI BstEII ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoRV FseI FspAI HindIII HpaI MluI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SrfI SwaI XhoI XmaI
ZraI