Restriction map for
pF1KE0071
(Linear) (Six-base) MAPSORT of: KIEE0071.seq Check: 9702 from: 1 to: 2169
KIEE0071.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 808 916 979 1378 1411 1439 1492 2169
Size: 808 108 63 399 33 28 53 677
Fragments arranged by size:
808 677 399 108 63 53 33 28
AccI GT'mk_AC
Cuts at: 0 266 2169
Size: 266 1903
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 258 2169
Size: 100 158 1911
Fragments arranged by size:
1911 158 100
AleI CACnn'nnGTG
Cuts at: 0 156 253 2169
Size: 156 97 1916
Fragments arranged by size:
1916 156 97
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1033 1065 1378 1410 2169
Size: 1033 32 313 32 759
Fragments arranged by size:
1033 759 313 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 486 864 1430 1554 2169
Size: 486 378 566 124 615
Fragments arranged by size:
615 566 486 378 124
ApaI G_GGCC'C
Cuts at: 0 656 1579 2169
Size: 656 923 590
Fragments arranged by size:
923 656 590
AvaI C'yCGr_G
Cuts at: 0 217 581 1036 1305 1523 1561 1572 2079
Size: 217 364 455 269 218 38 11 507
Cuts at: 2079 2169
Size: 90
Fragments arranged by size:
507 455 364 269 218 217 90 38
11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 753 786 948 981 1038 1071 2169
Size: 753 33 162 33 57 33 1098
Fragments arranged by size:
1098 753 162 57 33 33 33
BanI G'GyrC_C
Cuts at: 0 295 352 452 796 964 1249 1366 2169
Size: 295 57 100 344 168 285 117 803
Fragments arranged by size:
803 344 295 285 168 117 100 57
BanII G_rGCy'C
Cuts at: 0 64 656 1579 2008 2169
Size: 64 592 923 429 161
Fragments arranged by size:
923 592 429 161 64
BbeI G_GCGC'C
Cuts at: 0 299 2169
Size: 299 1870
BbvCI CC'TCA_GC
Cuts at: 0 1711 2169
Size: 1711 458
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 660 694 1017 1051 2169
Size: 660 34 323 34 1118
Fragments arranged by size:
1118 660 323 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 781 2169
Size: 781 1388
BglI GCCn_nnn'nGGC
Cuts at: 0 140 833 1067 1184 1298 1379 1412 2169
Size: 140 693 234 117 114 81 33 757
Fragments arranged by size:
757 693 234 140 117 114 81 33
Bme1580I G_kGCm'C
Cuts at: 0 355 656 1369 1440 1524 1579 2169
Size: 355 301 713 71 84 55 590
Fragments arranged by size:
713 590 355 301 84 71 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 579 2169
Size: 579 1590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 2169
Size: 78 2091
Bpu10I CC'TnA_GC
Cuts at: 0 1711 2169
Size: 1711 458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 496 1924 2169
Size: 175 321 1428 245
Fragments arranged by size:
1428 321 245 175
BsaI GGTCTCn'nnnn_
Cuts at: 0 1756 2169
Size: 1756 413
BsaHI Gr'CG_yC
Cuts at: 0 263 296 2169
Size: 263 33 1873
Fragments arranged by size:
1873 263 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 375 405 616 646 1033 1063 1302 1327
Size: 375 30 211 30 387 30 239 25
Cuts at: 1327 1332 1357 1378 1408 2169
Size: 5 25 21 30 761
Fragments arranged by size:
761 387 375 239 211 30 30 30
30 25 25 21 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 484 1335 1998 2032 2149 2169
Size: 484 851 663 34 117 20
Fragments arranged by size:
851 663 484 117 34 20
BseYI C'CCAG_C
Cuts at: 0 114 258 836 978 1000 1578 1677 1734
Size: 114 144 578 142 22 578 99 57
Cuts at: 1734 1908 2169
Size: 174 261
Fragments arranged by size:
578 578 261 174 144 142 114 99
57 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 472 645 753 858 894 1011 1383
Size: 213 259 173 108 105 36 117 372
Cuts at: 1383 1951 2169
Size: 568 218
Fragments arranged by size:
568 372 259 218 213 173 117 108
105 36
BsiEI CG_ry'CG
Cuts at: 0 501 2169
Size: 501 1668
BsiHKAI G_wGCw'C
Cuts at: 0 35 64 1144 1460 2169
Size: 35 29 1080 316 709
Fragments arranged by size:
1080 709 316 35 29
BsmI GAATG_Cn'
Cuts at: 0 730 2169
Size: 730 1439
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1750 2169
Size: 1750 419
Bsp1286I G_dGCh'C
Cuts at: 0 35 64 355 656 1144 1369 1440 1460
Size: 35 29 291 301 488 225 71 20
Cuts at: 1460 1524 1579 2008 2169
Size: 64 55 429 161
Fragments arranged by size:
488 429 301 291 225 161 71 64
55 35 29 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 494 808 916 979 1066 1378 1411 1439
Size: 494 314 108 63 87 312 33 28
Cuts at: 1439 1492 2169
Size: 53 677
Fragments arranged by size:
677 494 314 312 108 87 63 53
33 28
BsrFI r'CCGG_y
Cuts at: 0 752 947 1297 1727 2169
Size: 752 195 350 430 442
Fragments arranged by size:
752 442 430 350 195
BssHII G'CGCG_C
Cuts at: 0 14 2169
Size: 14 2155
BssSI C'ACGA_G
Cuts at: 0 1489 1927 2169
Size: 1489 438 242
Fragments arranged by size:
1489 438 242
BstAPI GCAn_nnn'nTGC
Cuts at: 0 11 131 1430 2169
Size: 11 120 1299 739
Fragments arranged by size:
1299 739 120 11
BstBI TT'CG_AA
Cuts at: 0 74 2169
Size: 74 2095
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1260 1631 2169
Size: 1260 371 538
Fragments arranged by size:
1260 538 371
BstYI r'GATC_y
Cuts at: 0 1032 2169
Size: 1032 1137
Bsu36I CC'TnA_GG
Cuts at: 0 2010 2169
Size: 2010 159
BtgI C'CryG_G
Cuts at: 0 135 792 1181 1863 2169
Size: 135 657 389 682 306
Fragments arranged by size:
682 657 389 306 135
BtsI GCAGTG_nn'
Cuts at: 0 823 1440 2169
Size: 823 617 729
Fragments arranged by size:
823 729 617
DraIII CAC_nnn'GTG
Cuts at: 0 1991 2169
Size: 1991 178
DrdI GACnn_nn'nnGTC
Cuts at: 0 1807 1957 2169
Size: 1807 150 212
Fragments arranged by size:
1807 212 150
EaeI y'GGCC_r
Cuts at: 0 346 440 498 597 1637 1778 2169
Size: 346 94 58 99 1040 141 391
Fragments arranged by size:
1040 391 346 141 99 94 58
EagI C'GGCC_G
Cuts at: 0 498 2169
Size: 498 1671
EarI CTCTTCn'nnn_
Cuts at: 0 1236 2169
Size: 1236 933
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1870 2169
Size: 1870 299
EcoICRI GAG'CTC
Cuts at: 0 62 2169
Size: 62 2107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 100 258 2169
Size: 78 22 158 1911
Fragments arranged by size:
1911 158 78 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1930 2169
Size: 1930 239
EcoO109I rG'GnC_Cy
Cuts at: 0 483 652 653 843 861 1537 1935 2027
Size: 483 169 1 190 18 676 398 92
Cuts at: 2027 2169
Size: 142
Fragments arranged by size:
676 483 398 190 169 142 92 18
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1875 1908 2169
Size: 1875 33 261
Fragments arranged by size:
1875 261 33
HaeII r_GCGC'y
Cuts at: 0 245 299 1880 2169
Size: 245 54 1581 289
Fragments arranged by size:
1581 289 245 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 460 492 1327 1359 2169
Size: 460 32 835 32 810
Fragments arranged by size:
835 810 460 32 32
HincII GTy'rAC
Cuts at: 0 99 267 2169
Size: 99 168 1902
Fragments arranged by size:
1902 168 99
KasI G'GCGC_C
Cuts at: 0 295 2169
Size: 295 1874
MscI TGG'CCA
Cuts at: 0 442 1780 2169
Size: 442 1338 389
Fragments arranged by size:
1338 442 389
MslI CAynn'nnrTG
Cuts at: 0 156 253 1505 2169
Size: 156 97 1252 664
Fragments arranged by size:
1252 664 156 97
MspA1I CmG'CkG
Cuts at: 0 137 497 1169 1175 1289 1582 1690 1912
Size: 137 360 672 6 114 293 108 222
Cuts at: 1912 2169
Size: 257
Fragments arranged by size:
672 360 293 257 222 137 114 108
6
NaeI GCC'GGC
Cuts at: 0 949 1299 2169
Size: 949 350 870
Fragments arranged by size:
949 870 350
NarI GG'CG_CC
Cuts at: 0 296 2169
Size: 296 1873
NcoI C'CATG_G
Cuts at: 0 792 2169
Size: 792 1377
NgoMIV G'CCGG_C
Cuts at: 0 947 1297 2169
Size: 947 350 872
Fragments arranged by size:
947 872 350
NotI GC'GGCC_GC
Cuts at: 0 498 2169
Size: 498 1671
PasI CC'CwG_GG
Cuts at: 0 1631 2001 2169
Size: 1631 370 168
Fragments arranged by size:
1631 370 168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1033 1065 1378 1410 2169
Size: 1033 32 313 32 759
Fragments arranged by size:
1033 759 313 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 483 861 1537 1935 2027 2169
Size: 483 378 676 398 92 142
Fragments arranged by size:
676 483 398 378 142 92
PspOMI G'GGCC_C
Cuts at: 0 652 1575 2169
Size: 652 923 594
Fragments arranged by size:
923 652 594
PstI C_TGCA'G
Cuts at: 0 89 1228 1435 1721 2169
Size: 89 1139 207 286 448
Fragments arranged by size:
1139 448 286 207 89
PvuII CAG'CTG
Cuts at: 0 1169 2169
Size: 1169 1000
SacI G_AGCT'C
Cuts at: 0 64 2169
Size: 64 2105
SacII CC_GC'GG
Cuts at: 0 138 2169
Size: 138 2031
SalI G'TCGA_C
Cuts at: 0 265 2169
Size: 265 1904
SanDI GG'GwC_CC
Cuts at: 0 1537 2027 2169
Size: 1537 490 142
Fragments arranged by size:
1537 490 142
SapI GCTCTTCn'nnn_
Cuts at: 0 1236 2169
Size: 1236 933
ScaI AGT'ACT
Cuts at: 0 517 2169
Size: 517 1652
SfcI C'TryA_G
Cuts at: 0 85 1224 1431 1717 2169
Size: 85 1139 207 286 452
Fragments arranged by size:
1139 452 286 207 85
SfoI GGC'GCC
Cuts at: 0 297 2169
Size: 297 1872
SmaI CCC'GGG
Cuts at: 0 583 1574 2169
Size: 583 991 595
Fragments arranged by size:
991 595 583
SmlI C'TyrA_G
Cuts at: 0 190 511 1036 1939 2169
Size: 190 321 525 903 230
Fragments arranged by size:
903 525 321 230 190
StyI C'CwwG_G
Cuts at: 0 792 2169
Size: 792 1377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 460 1623 2169
Size: 460 1163 546
Fragments arranged by size:
1163 546 460
TatI w'GTAC_w
Cuts at: 0 515 2169
Size: 515 1654
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 375 407 616 648 715 747 1302 1334
Size: 375 32 209 32 67 32 555 32
Cuts at: 1334 1419 1451 2169
Size: 85 32 718
Fragments arranged by size:
718 555 375 209 85 67 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 607 975 1773 2169
Size: 607 368 798 396
Fragments arranged by size:
798 607 396 368
XhoI C'TCGA_G
Cuts at: 0 1036 2169
Size: 1036 1133
XmaI C'CCGG_G
Cuts at: 0 581 1572 2169
Size: 581 991 597
Fragments arranged by size:
991 597 581
XmnI GAAnn'nnTTC
Cuts at: 0 1390 1891 2169
Size: 1390 501 278
Fragments arranged by size:
1390 501 278
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI ApaI AvaI
BaeI BanI BanII BbeI BbvCI BcgI BciVI BglI
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII KasI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI PasI PpiI PpuMI PspOMI PstI PvuII
SacI SacII SalI SanDI SapI ScaI SfcI SfoI
SmaI SmlI StyI TaqII TatI TstI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BamHI
BbsI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BsaAI BsaBI BsaWI BsiWI BspEI BspHI BsrBI BsrDI
BsrGI BstEII BstZ17I BtgZI ClaI CspCI DraI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MmeI NdeI NheI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SbfI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI ZraI