Restriction map for
pF1KE0065
(Linear) (Six-base) MAPSORT of: KIEE0065.seq Check: 5932 from: 1 to: 3090
KIEE0065.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 0 1174 3090
Size: 1174 1916
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1259 1526 3090
Size: 853 406 267 1564
Fragments arranged by size:
1564 853 406 267
AflIII A'CryG_T
Cuts at: 0 578 3000 3090
Size: 578 2422 90
Fragments arranged by size:
2422 578 90
AhdI GACnn_n'nnGTC
Cuts at: 0 582 1485 3090
Size: 582 903 1605
Fragments arranged by size:
1605 903 582
AlwNI CAG_nnn'CTG
Cuts at: 0 1044 3090
Size: 1044 2046
ApaI G_GGCC'C
Cuts at: 0 1518 3033 3090
Size: 1518 1515 57
Fragments arranged by size:
1518 1515 57
ApoI r'AATT_y
Cuts at: 0 34 127 791 825 1572 2604 2618 3090
Size: 34 93 664 34 747 1032 14 472
Fragments arranged by size:
1032 747 664 472 93 34 34 14
AvaI C'yCGr_G
Cuts at: 0 1061 2467 3090
Size: 1061 1406 623
Fragments arranged by size:
1406 1061 623
BanI G'GyrC_C
Cuts at: 0 374 2011 2462 3090
Size: 374 1637 451 628
Fragments arranged by size:
1637 628 451 374
BanII G_rGCy'C
Cuts at: 0 740 1518 1532 2859 3033 3090
Size: 740 778 14 1327 174 57
Fragments arranged by size:
1327 778 740 174 57 14
BbeI G_GCGC'C
Cuts at: 0 2466 3090
Size: 2466 624
BbsI GAAGACnn'nnnn_
Cuts at: 0 2739 3090
Size: 2739 351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1602 1636 1967 2001 3090
Size: 1602 34 331 34 1089
Fragments arranged by size:
1602 1089 331 34 34
BglII A'GATC_T
Cuts at: 0 1018 1185 1315 1981 2474 2481 3090
Size: 1018 167 130 666 493 7 609
Fragments arranged by size:
1018 666 609 493 167 130 7
BlpI GC'TnA_GC
Cuts at: 0 610 1624 3090
Size: 610 1014 1466
Fragments arranged by size:
1466 1014 610
Bme1580I G_kGCm'C
Cuts at: 0 1473 1518 2926 3033 3090
Size: 1473 45 1408 107 57
Fragments arranged by size:
1473 1408 107 57 45
BmgBI CAC'GTC
Cuts at: 0 1672 2164 3090
Size: 1672 492 926
Fragments arranged by size:
1672 926 492
BmrI ACTGGGnnnn_n'
Cuts at: 0 1416 1526 1795 3090
Size: 1416 110 269 1295
Fragments arranged by size:
1416 1295 269 110
BmtI G_CTAG'C
Cuts at: 0 2435 3090
Size: 2435 655
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1972 2493 3090
Size: 1972 521 597
Fragments arranged by size:
1972 597 521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 1136 1946 1967 1986 2686 2859 2884
Size: 656 480 810 21 19 700 173 25
Cuts at: 2884 2917 3090
Size: 33 173
Fragments arranged by size:
810 700 656 480 173 173 33 25
21 19
Bpu10I CC'TnA_GC
Cuts at: 0 2635 3090
Size: 2635 455
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1285 1905 2296 3090
Size: 184 1101 620 391 794
Fragments arranged by size:
1101 794 620 391 184
BsaI GGTCTCn'nnnn_
Cuts at: 0 1081 1990 3090
Size: 1081 909 1100
Fragments arranged by size:
1100 1081 909
BsaHI Gr'CG_yC
Cuts at: 0 2463 3090
Size: 2463 627
BsaWI w'CCGG_w
Cuts at: 0 740 3090
Size: 740 2350
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2121 2151 3090
Size: 2121 30 939
Fragments arranged by size:
2121 939 30
BseYI C'CCAG_C
Cuts at: 0 843 1363 1811 1940 2969 3090
Size: 843 520 448 129 1029 121
Fragments arranged by size:
1029 843 520 448 129 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1120 1742 3090
Size: 1120 622 1348
Fragments arranged by size:
1348 1120 622
BsiHKAI G_wGCw'C
Cuts at: 0 1727 3090
Size: 1727 1363
BsmI GAATG_Cn'
Cuts at: 0 1830 2263 3075 3090
Size: 1830 433 812 15
Fragments arranged by size:
1830 812 433 15
Bsp1286I G_dGCh'C
Cuts at: 0 740 1473 1518 1532 1727 2859 2926 3033
Size: 740 733 45 14 195 1132 67 107
Cuts at: 3033 3090
Size: 57
Fragments arranged by size:
1132 740 733 195 107 67 57 45
14
BspHI T'CATG_A
Cuts at: 0 2058 3090
Size: 2058 1032
BsrFI r'CCGG_y
Cuts at: 0 985 3090
Size: 985 2105
BssSI C'ACGA_G
Cuts at: 0 1611 3090
Size: 1611 1479
BstEII G'GTnAC_C
Cuts at: 0 2815 3090
Size: 2815 275
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1872 2459 2647 3090
Size: 1872 587 188 443
Fragments arranged by size:
1872 587 443 188
BstYI r'GATC_y
Cuts at: 0 41 1018 1185 1315 1981 2474 2481 2565
Size: 41 977 167 130 666 493 7 84
Cuts at: 2565 3090
Size: 525
Fragments arranged by size:
977 666 525 493 167 130 84 41
7
BtgI C'CryG_G
Cuts at: 0 572 732 1464 2272 3090
Size: 572 160 732 808 818
Fragments arranged by size:
818 808 732 572 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 629 3090
Size: 629 2461
BtsI GCAGTG_nn'
Cuts at: 0 1132 1369 2512 3090
Size: 1132 237 1143 578
Fragments arranged by size:
1143 1132 578 237
DraIII CAC_nnn'GTG
Cuts at: 0 1477 2840 3090
Size: 1477 1363 250
Fragments arranged by size:
1477 1363 250
EarI CTCTTCn'nnn_
Cuts at: 0 2022 2541 3090
Size: 2022 519 549
Fragments arranged by size:
2022 549 519
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 853 1136 1259 1526 1946 1967 1986
Size: 656 197 283 123 267 420 21 19
Cuts at: 1986 2686 2859 2884 2917 3090
Size: 700 173 25 33 173
Fragments arranged by size:
700 656 420 283 267 197 173 173
123 33 25 21 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1277 1443 2638 3090
Size: 1277 166 1195 452
Fragments arranged by size:
1277 1195 452 166
EcoO109I rG'GnC_Cy
Cuts at: 0 1514 3029 3090
Size: 1514 1515 61
Fragments arranged by size:
1515 1514 61
EcoRI G'AATT_C
Cuts at: 0 1572 3090
Size: 1572 1518
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 273 959 1016 3090
Size: 273 686 57 2074
Fragments arranged by size:
2074 686 273 57
FspI TGC'GCA
Cuts at: 0 2518 3090
Size: 2518 572
HaeII r_GCGC'y
Cuts at: 0 557 2466 3090
Size: 557 1909 624
Fragments arranged by size:
1909 624 557
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 568 600 1471 1503 1973 2005 2053 2085
Size: 568 32 871 32 470 32 48 32
Cuts at: 2085 2466 2498 3090
Size: 381 32 592
Fragments arranged by size:
871 592 568 470 381 48 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 756 2198 3090
Size: 756 1442 892
Fragments arranged by size:
1442 892 756
HindIII A'AGCT_T
Cuts at: 0 706 1092 1881 3090
Size: 706 386 789 1209
Fragments arranged by size:
1209 789 706 386
HpaI GTT'AAC
Cuts at: 0 756 3090
Size: 756 2334
KasI G'GCGC_C
Cuts at: 0 2462 3090
Size: 2462 628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 818 2411 3090
Size: 220 598 1593 679
Fragments arranged by size:
1593 679 598 220
MslI CAynn'nnrTG
Cuts at: 0 383 437 2072 2917 3068 3090
Size: 383 54 1635 845 151 22
Fragments arranged by size:
1635 845 383 151 54 22
MspA1I CmG'CkG
Cuts at: 0 751 847 2706 2960 3090
Size: 751 96 1859 254 130
Fragments arranged by size:
1859 751 254 130 96
NaeI GCC'GGC
Cuts at: 0 987 3090
Size: 987 2103
NarI GG'CG_CC
Cuts at: 0 2463 3090
Size: 2463 627
NcoI C'CATG_G
Cuts at: 0 732 1464 2272 3090
Size: 732 732 808 818
Fragments arranged by size:
818 808 732 732
NgoMIV G'CCGG_C
Cuts at: 0 985 3090
Size: 985 2105
NheI G'CTAG_C
Cuts at: 0 2431 3090
Size: 2431 659
NspI r_CATG'y
Cuts at: 0 582 3090
Size: 582 2508
PasI CC'CwG_GG
Cuts at: 0 1285 3090
Size: 1285 1805
PciI A'CATG_T
Cuts at: 0 578 3090
Size: 578 2512
PflMI CCAn_nnn'nTGG
Cuts at: 0 524 2716 3090
Size: 524 2192 374
Fragments arranged by size:
2192 524 374
PfoI T'CCnGG_A
Cuts at: 0 633 3090
Size: 633 2457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1318 1350 3090
Size: 1318 32 1740
Fragments arranged by size:
1740 1318 32
PshAI GACnn'nnGTC
Cuts at: 0 1073 1670 3090
Size: 1073 597 1420
Fragments arranged by size:
1420 1073 597
PspOMI G'GGCC_C
Cuts at: 0 1514 3029 3090
Size: 1514 1515 61
Fragments arranged by size:
1515 1514 61
PstI C_TGCA'G
Cuts at: 0 1376 2489 3090
Size: 1376 1113 601
Fragments arranged by size:
1376 1113 601
PvuII CAG'CTG
Cuts at: 0 751 847 2706 2960 3090
Size: 751 96 1859 254 130
Fragments arranged by size:
1859 751 254 130 96
ScaI AGT'ACT
Cuts at: 0 996 2897 3090
Size: 996 1901 193
Fragments arranged by size:
1901 996 193
SfcI C'TryA_G
Cuts at: 0 714 1372 1443 2485 3090
Size: 714 658 71 1042 605
Fragments arranged by size:
1042 714 658 605 71
SfoI GGC'GCC
Cuts at: 0 2464 3090
Size: 2464 626
SmaI CCC'GGG
Cuts at: 0 1063 3090
Size: 1063 2027
SmlI C'TyrA_G
Cuts at: 0 199 1300 1884 2311 3090
Size: 199 1101 584 427 779
Fragments arranged by size:
1101 779 584 427 199
SspI AAT'ATT
Cuts at: 0 1260 2292 3090
Size: 1260 1032 798
Fragments arranged by size:
1260 1032 798
StuI AGG'CCT
Cuts at: 0 1055 2644 3090
Size: 1055 1589 446
Fragments arranged by size:
1589 1055 446
StyI C'CwwG_G
Cuts at: 0 45 732 770 786 1451 1464 1865 2125
Size: 45 687 38 16 665 13 401 260
Cuts at: 2125 2272 3090
Size: 147 818
Fragments arranged by size:
818 687 665 401 260 147 45 38
16 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 521 1977 3090
Size: 521 1456 1113
Fragments arranged by size:
1456 1113 521
TatI w'GTAC_w
Cuts at: 0 78 254 994 2895 3090
Size: 78 176 740 1901 195
Fragments arranged by size:
1901 740 195 176 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 557 589 2542 2574 2921 2953 3090
Size: 557 32 1953 32 347 32 137
Fragments arranged by size:
1953 557 347 137 32 32 32
XmaI C'CCGG_G
Cuts at: 0 1061 3090
Size: 1061 2029
XmnI GAAnn'nnTTC
Cuts at: 0 233 709 3090
Size: 233 476 2381
Fragments arranged by size:
2381 476 233
Enzymes that do cut:
AccI AcuI AflIII AhdI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbsI BcgI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BsrFI BssSI BstEII BstXI BstYI BtgI BtgZI
BtsI DraIII EarI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI MmeI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PfoI PpiI PshAI PspOMI PstI
PvuII ScaI SfcI SfoI SmaI SmlI SspI StuI
StyI TaqII TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BciVI BclI BfrBI BglI BsaAI BsaBI
BseRI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI
BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EaeI EagI EciI EcoICRI EcoRV FseI
FspAI KpnI MfeI MluI MscI NdeI NotI NruI
NsiI PacI PmeI PmlI PpuMI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SwaI Tth111I XbaI XcmI XhoI ZraI