Restriction map for
pF1KE0062
(Linear) (Six-base) MAPSORT of: KIEE0062.seq Check: 3046 from: 1 to: 1902
KIEE0062.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 0 629 997 1902
Size: 629 368 905
Fragments arranged by size:
905 629 368
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 1754 1902
Size: 683 1071 148
Fragments arranged by size:
1071 683 148
AflIII A'CryG_T
Cuts at: 0 254 297 467 1170 1902
Size: 254 43 170 703 732
Fragments arranged by size:
732 703 254 170 43
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1790 1822 1902
Size: 1790 32 80
Fragments arranged by size:
1790 80 32
AlwNI CAG_nnn'CTG
Cuts at: 0 737 855 1902
Size: 737 118 1047
Fragments arranged by size:
1047 737 118
ApoI r'AATT_y
Cuts at: 0 1225 1848 1902
Size: 1225 623 54
Fragments arranged by size:
1225 623 54
AvaI C'yCGr_G
Cuts at: 0 91 610 1902
Size: 91 519 1292
Fragments arranged by size:
1292 519 91
BamHI G'GATC_C
Cuts at: 0 37 1203 1902
Size: 37 1166 699
Fragments arranged by size:
1166 699 37
BanI G'GyrC_C
Cuts at: 0 209 1902
Size: 209 1693
BanII G_rGCy'C
Cuts at: 0 623 1537 1902
Size: 623 914 365
Fragments arranged by size:
914 623 365
BbsI GAAGACnn'nnnn_
Cuts at: 0 360 1902
Size: 360 1542
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1088 1122 1902
Size: 1088 34 780
Fragments arranged by size:
1088 780 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 482 1902
Size: 482 1420
BmrI ACTGGGnnnn_n'
Cuts at: 0 626 1902
Size: 626 1276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 1902
Size: 200 1702
BsaBI GATnn'nnATC
Cuts at: 0 428 1902
Size: 428 1474
BsaHI Gr'CG_yC
Cuts at: 0 816 1902
Size: 816 1086
BsaWI w'CCGG_w
Cuts at: 0 200 1902
Size: 200 1702
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1790 1820 1902
Size: 1790 30 82
Fragments arranged by size:
1790 82 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 146 197 1902
Size: 146 51 1705
Fragments arranged by size:
1705 146 51
BseYI C'CCAG_C
Cuts at: 0 1887 1902
Size: 1887 15
BsiHKAI G_wGCw'C
Cuts at: 0 806 1254 1537 1902
Size: 806 448 283 365
Fragments arranged by size:
806 448 365 283
Bsp1286I G_dGCh'C
Cuts at: 0 623 806 1254 1537 1902
Size: 623 183 448 283 365
Fragments arranged by size:
623 448 365 283 183
BspHI T'CATG_A
Cuts at: 0 519 1212 1902
Size: 519 693 690
Fragments arranged by size:
693 690 519
BstBI TT'CG_AA
Cuts at: 0 1629 1902
Size: 1629 273
BstYI r'GATC_y
Cuts at: 0 37 451 1203 1902
Size: 37 414 752 699
Fragments arranged by size:
752 699 414 37
BstZ17I GTA'TAC
Cuts at: 0 630 1902
Size: 630 1272
BtgI C'CryG_G
Cuts at: 0 1085 1902
Size: 1085 817
BtsI GCAGTG_nn'
Cuts at: 0 756 804 1902
Size: 756 48 1098
Fragments arranged by size:
1098 756 48
ClaI AT'CG_AT
Cuts at: 0 1319 1902
Size: 1319 583
EarI CTCTTCn'nnn_
Cuts at: 0 79 1773 1902
Size: 79 1694 129
Fragments arranged by size:
1694 129 79
EcoICRI GAG'CTC
Cuts at: 0 1535 1902
Size: 1535 367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 683 1754 1902
Size: 200 483 1071 148
Fragments arranged by size:
1071 483 200 148
EcoRV GAT'ATC
Cuts at: 0 915 1636 1902
Size: 915 721 266
Fragments arranged by size:
915 721 266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1321 1902
Size: 1321 581
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1160 1192 1902
Size: 1160 32 710
Fragments arranged by size:
1160 710 32
MslI CAynn'nnrTG
Cuts at: 0 518 1361 1902
Size: 518 843 541
Fragments arranged by size:
843 541 518
MspA1I CmG'CkG
Cuts at: 0 679 855 1150 1236 1902
Size: 679 176 295 86 666
Fragments arranged by size:
679 666 295 176 86
NcoI C'CATG_G
Cuts at: 0 1085 1902
Size: 1085 817
NspI r_CATG'y
Cuts at: 0 258 301 402 441 471 979 1174 1345
Size: 258 43 101 39 30 508 195 171
Cuts at: 1345 1902
Size: 557
Fragments arranged by size:
557 508 258 195 171 101 43 39
30
PciI A'CATG_T
Cuts at: 0 254 297 467 1170 1902
Size: 254 43 170 703 732
Fragments arranged by size:
732 703 254 170 43
PflMI CCAn_nnn'nTGG
Cuts at: 0 855 1744 1902
Size: 855 889 158
Fragments arranged by size:
889 855 158
PfoI T'CCnGG_A
Cuts at: 0 1199 1902
Size: 1199 703
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1790 1822 1902
Size: 1790 32 80
Fragments arranged by size:
1790 80 32
PspXI vC'TCGA_Gb
Cuts at: 0 91 1902
Size: 91 1811
PstI C_TGCA'G
Cuts at: 0 678 1902
Size: 678 1224
PvuII CAG'CTG
Cuts at: 0 679 855 1150 1236 1902
Size: 679 176 295 86 666
Fragments arranged by size:
679 666 295 176 86
SacI G_AGCT'C
Cuts at: 0 1537 1902
Size: 1537 365
ScaI AGT'ACT
Cuts at: 0 569 1902
Size: 569 1333
SfcI C'TryA_G
Cuts at: 0 674 1902
Size: 674 1228
SmlI C'TyrA_G
Cuts at: 0 91 610 1902
Size: 91 519 1292
Fragments arranged by size:
1292 519 91
SpeI A'CTAG_T
Cuts at: 0 1720 1902
Size: 1720 182
SphI G_CATG'C
Cuts at: 0 441 1345 1902
Size: 441 904 557
Fragments arranged by size:
904 557 441
SspI AAT'ATT
Cuts at: 0 556 1188 1902
Size: 556 632 714
Fragments arranged by size:
714 632 556
StuI AGG'CCT
Cuts at: 0 1573 1902
Size: 1573 329
StyI C'CwwG_G
Cuts at: 0 866 1085 1568 1640 1902
Size: 866 219 483 72 262
Fragments arranged by size:
866 483 262 219 72
TatI w'GTAC_w
Cuts at: 0 567 1902
Size: 567 1335
XhoI C'TCGA_G
Cuts at: 0 91 610 1902
Size: 91 519 1292
Fragments arranged by size:
1292 519 91
XmnI GAAnn'nnTTC
Cuts at: 0 1268 1902
Size: 1268 634
Enzymes that do cut:
AccI AcuI AflIII AloI AlwNI ApoI AvaI BamHI
BanI BanII BbsI BcgI BciVI BmrI BpmI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI
BstBI BstYI BstZ17I BtgI BtsI ClaI EarI EcoICRI
Eco57MI EcoRV FalI Hin4I MslI MspA1I NcoI NspI
PciI PflMI PfoI PpiI PspXI PstI PvuII SacI
ScaI SfcI SmlI SpeI SphI SspI StuI StyI
TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
Bsu36I BtgZI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpuMI PshAI PsiI
PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SrfI SwaI TaqII TstI Tth111I XbaI XcmI XmaI
ZraI