Restriction map for
pF1KE0058
(Linear) (Six-base) MAPSORT of: KIEE0058.seq Check: 2208 from: 1 to: 2277
KIEE0058.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 618 2277
Size: 618 1659
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 812 1402 2277
Size: 797 15 590 875
Fragments arranged by size:
875 797 590 15
AflIII A'CryG_T
Cuts at: 0 1529 2277
Size: 1529 748
AlwNI CAG_nnn'CTG
Cuts at: 0 349 610 2277
Size: 349 261 1667
Fragments arranged by size:
1667 349 261
ApaI G_GGCC'C
Cuts at: 0 2110 2277
Size: 2110 167
ApoI r'AATT_y
Cuts at: 0 339 833 1168 1224 1822 2277
Size: 339 494 335 56 598 455
Fragments arranged by size:
598 494 455 339 335 56
AvaI C'yCGr_G
Cuts at: 0 1580 2277
Size: 1580 697
BanI G'GyrC_C
Cuts at: 0 798 1973 2277
Size: 798 1175 304
Fragments arranged by size:
1175 798 304
BanII G_rGCy'C
Cuts at: 0 539 1194 2110 2277
Size: 539 655 916 167
Fragments arranged by size:
916 655 539 167
BbsI GAAGACnn'nnnn_
Cuts at: 0 517 2277
Size: 517 1760
BbvCI CC'TCA_GC
Cuts at: 0 1859 2277
Size: 1859 418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1596 1630 2277
Size: 1596 34 647
Fragments arranged by size:
1596 647 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 556 2277
Size: 556 1721
BclI T'GATC_A
Cuts at: 0 2 2277
Size: 2 2275
BfrBI ATG'CAT
Cuts at: 0 1613 2277
Size: 1613 664
BglII A'GATC_T
Cuts at: 0 1024 2277
Size: 1024 1253
Bme1580I G_kGCm'C
Cuts at: 0 801 2110 2277
Size: 801 1309 167
Fragments arranged by size:
1309 801 167
BmrI ACTGGGnnnn_n'
Cuts at: 0 51 515 2277
Size: 51 464 1762
Fragments arranged by size:
1762 464 51
BmtI G_CTAG'C
Cuts at: 0 752 2277
Size: 752 1525
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 419 711 975 1427 2277
Size: 404 15 292 264 452 850
Fragments arranged by size:
850 452 404 292 264 15
Bpu10I CC'TnA_GC
Cuts at: 0 1119 1859 2277
Size: 1119 740 418
Fragments arranged by size:
1119 740 418
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1763 2277
Size: 1763 514
BsaI GGTCTCn'nnnn_
Cuts at: 0 104 1474 2277
Size: 104 1370 803
Fragments arranged by size:
1370 803 104
BsaBI GATnn'nnATC
Cuts at: 0 1239 2277
Size: 1239 1038
BsaHI Gr'CG_yC
Cuts at: 0 43 2277
Size: 43 2234
BsaWI w'CCGG_w
Cuts at: 0 372 2277
Size: 372 1905
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 84 114 2277
Size: 84 30 2163
Fragments arranged by size:
2163 84 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 668 749 1300 1513 1764 2277
Size: 668 81 551 213 251 513
Fragments arranged by size:
668 551 513 251 213 81
BseYI C'CCAG_C
Cuts at: 0 1626 2152 2277
Size: 1626 526 125
Fragments arranged by size:
1626 526 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 1364 2277
Size: 596 768 913
Fragments arranged by size:
913 768 596
BsiEI CG_ry'CG
Cuts at: 0 303 2277
Size: 303 1974
BsiHKAI G_wGCw'C
Cuts at: 0 1301 2277
Size: 1301 976
BsmI GAATG_Cn'
Cuts at: 0 582 1055 2277
Size: 582 473 1222
Fragments arranged by size:
1222 582 473
BsmBI CGTCTCn'nnnn_
Cuts at: 0 638 2277
Size: 638 1639
Bsp1286I G_dGCh'C
Cuts at: 0 539 801 1194 1301 2110 2277
Size: 539 262 393 107 809 167
Fragments arranged by size:
809 539 393 262 167 107
BspEI T'CCGG_A
Cuts at: 0 372 2277
Size: 372 1905
BspHI T'CATG_A
Cuts at: 0 1931 2277
Size: 1931 346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 618 830 2277
Size: 618 212 1447
Fragments arranged by size:
1447 618 212
BsrDI GCAATG_nn'
Cuts at: 0 1460 2277
Size: 1460 817
BsrFI r'CCGG_y
Cuts at: 0 1651 2277
Size: 1651 626
BsrGI T'GTAC_A
Cuts at: 0 1043 2277
Size: 1043 1234
BstEII G'GTnAC_C
Cuts at: 0 1413 2277
Size: 1413 864
BstXI CCAn_nnnn'nTGG
Cuts at: 0 892 1451 2277
Size: 892 559 826
Fragments arranged by size:
892 826 559
BstYI r'GATC_y
Cuts at: 0 1024 1240 2277
Size: 1024 216 1037
Fragments arranged by size:
1037 1024 216
BtgI C'CryG_G
Cuts at: 0 891 2277
Size: 891 1386
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1253 1599 2277
Size: 1253 346 678
Fragments arranged by size:
1253 678 346
BtsI GCAGTG_nn'
Cuts at: 0 43 383 2035 2105 2277
Size: 43 340 1652 70 172
Fragments arranged by size:
1652 340 172 70 43
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 674 709 2277
Size: 674 35 1568
Fragments arranged by size:
1568 674 35
DraIII CAC_nnn'GTG
Cuts at: 0 1368 2277
Size: 1368 909
EaeI y'GGCC_r
Cuts at: 0 61 1014 1510 1718 2277
Size: 61 953 496 208 559
Fragments arranged by size:
953 559 496 208 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 671 797 2277
Size: 671 126 1480
Fragments arranged by size:
1480 671 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 419 711 797 812 975 1402 1427
Size: 404 15 292 86 15 163 427 25
Cuts at: 1427 2277
Size: 850
Fragments arranged by size:
850 427 404 292 163 86 25 15
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1480 2277
Size: 1480 797
EcoO109I rG'GnC_Cy
Cuts at: 0 241 1330 1438 2107 2277
Size: 241 1089 108 669 170
Fragments arranged by size:
1089 669 241 170 108
EcoRI G'AATT_C
Cuts at: 0 339 833 2277
Size: 339 494 1444
Fragments arranged by size:
1444 494 339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 974 2277
Size: 974 1303
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 160 192 245 268 277 300
Size: 70 32 58 32 53 23 9 23
Cuts at: 300 973 1005 1931 1963 2277
Size: 673 32 926 32 314
Fragments arranged by size:
926 673 314 70 58 53 32 32
32 32 23 23 9
HindIII A'AGCT_T
Cuts at: 0 956 2277
Size: 956 1321
MluI A'CGCG_T
Cuts at: 0 1529 2277
Size: 1529 748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 283 1382 1789 2277
Size: 218 65 1099 407 488
Fragments arranged by size:
1099 488 407 218 65
MscI TGG'CCA
Cuts at: 0 63 1016 1512 1720 2277
Size: 63 953 496 208 557
Fragments arranged by size:
953 557 496 208 63
MslI CAynn'nnrTG
Cuts at: 0 1762 2277
Size: 1762 515
MspA1I CmG'CkG
Cuts at: 0 1626 2277
Size: 1626 651
NcoI C'CATG_G
Cuts at: 0 891 2277
Size: 891 1386
NheI G'CTAG_C
Cuts at: 0 748 2277
Size: 748 1529
NsiI A_TGCA'T
Cuts at: 0 1615 2277
Size: 1615 662
NspI r_CATG'y
Cuts at: 0 55 1767 2277
Size: 55 1712 510
Fragments arranged by size:
1712 510 55
PasI CC'CwG_GG
Cuts at: 0 790 2277
Size: 790 1487
PflMI CCAn_nnn'nTGG
Cuts at: 0 1368 2277
Size: 1368 909
PpuMI rG'GwC_Cy
Cuts at: 0 241 1438 2277
Size: 241 1197 839
Fragments arranged by size:
1197 839 241
PspOMI G'GGCC_C
Cuts at: 0 2106 2277
Size: 2106 171
PstI C_TGCA'G
Cuts at: 0 140 2277
Size: 140 2137
PvuII CAG'CTG
Cuts at: 0 1626 2277
Size: 1626 651
ScaI AGT'ACT
Cuts at: 0 1215 2277
Size: 1215 1062
SexAI A'CCwGG_T
Cuts at: 0 1409 1475 2277
Size: 1409 66 802
Fragments arranged by size:
1409 802 66
SfcI C'TryA_G
Cuts at: 0 123 136 658 1209 2277
Size: 123 13 522 551 1068
Fragments arranged by size:
1068 551 522 123 13
SmlI C'TyrA_G
Cuts at: 0 1778 2277
Size: 1778 499
SpeI A'CTAG_T
Cuts at: 0 291 1662 2277
Size: 291 1371 615
Fragments arranged by size:
1371 615 291
SphI G_CATG'C
Cuts at: 0 55 2277
Size: 55 2222
StyI C'CwwG_G
Cuts at: 0 692 891 1434 2277
Size: 692 199 543 843
Fragments arranged by size:
843 692 543 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 86 266 1334 1447 2277
Size: 86 180 1068 113 830
Fragments arranged by size:
1068 830 180 113 86
TatI w'GTAC_w
Cuts at: 0 1043 1213 1262 1845 1986 2277
Size: 1043 170 49 583 141 291
Fragments arranged by size:
1043 583 291 170 141 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 84 116 852 884 1430 1462 2027 2059
Size: 84 32 736 32 546 32 565 32
Cuts at: 2059 2060 2092 2277
Size: 1 32 185
Fragments arranged by size:
736 565 546 185 84 32 32 32
32 32 1
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1365 2277
Size: 1365 912
XmnI GAAnn'nnTTC
Cuts at: 0 958 1916 2277
Size: 958 958 361
Fragments arranged by size:
958 958 361
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI ApoI AvaI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglII
Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BsrGI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraIII EaeI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I HindIII MluI MmeI MscI MslI MspA1I NcoI
NheI NsiI NspI PasI PflMI PpuMI PspOMI PstI
PvuII ScaI SexAI SfcI SmlI SpeI SphI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BglI BlpI BmgBI BplI BsaAI
BsiWI BsrBI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
ClaI DraI DrdI EagI EarI EcoICRI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
NaeI NarI NdeI NgoMIV NotI NruI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmaI ZraI