Restriction map for
pF1KE0053
(Linear) (Six-base) MAPSORT of: KIEE0053.seq Check: 5844 from: 1 to: 4704
KIEE0053.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AatII G_ACGT'C
Cuts at: 0 914 4214 4704
Size: 914 3300 490
Fragments arranged by size:
3300 914 490
AccI GT'mk_AC
Cuts at: 0 3299 4066 4704
Size: 3299 767 638
Fragments arranged by size:
3299 767 638
Acc65I G'GTAC_C
Cuts at: 0 524 4704
Size: 524 4180
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 975 3134 3736 3914 4288 4704
Size: 297 678 2159 602 178 374 416
Fragments arranged by size:
2159 678 602 416 374 297 178
AflII C'TTAA_G
Cuts at: 0 1198 3723 4704
Size: 1198 2525 981
Fragments arranged by size:
2525 1198 981
AleI CACnn'nnGTG
Cuts at: 0 454 547 814 4704
Size: 454 93 267 3890
Fragments arranged by size:
3890 454 267 93
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 340 454 832 1530 4704
Size: 340 114 378 698 3174
Fragments arranged by size:
3174 698 378 340 114
AlwNI CAG_nnn'CTG
Cuts at: 0 657 1909 2105 4207 4704
Size: 657 1252 196 2102 497
Fragments arranged by size:
2102 1252 657 497 196
ApaLI G'TGCA_C
Cuts at: 0 1440 4704
Size: 1440 3264
ApoI r'AATT_y
Cuts at: 0 1218 1346 1523 1743 2158 2331 2585 3583
Size: 1218 128 177 220 415 173 254 998
Cuts at: 3583 3921 4504 4540 4568 4704
Size: 338 583 36 28 136
Fragments arranged by size:
1218 998 583 415 338 254 220 177
173 136 128 36 28
AvaI C'yCGr_G
Cuts at: 0 301 355 2063 4704
Size: 301 54 1708 2641
Fragments arranged by size:
2641 1708 301 54
BamHI G'GATC_C
Cuts at: 0 2236 3889 4704
Size: 2236 1653 815
Fragments arranged by size:
2236 1653 815
BanI G'GyrC_C
Cuts at: 0 21 279 394 448 524 662 1141 1421
Size: 21 258 115 54 76 138 479 280
Cuts at: 1421 4704
Size: 3283
Fragments arranged by size:
3283 479 280 258 138 115 76 54
21
BanII G_rGCy'C
Cuts at: 0 113 481 1634 2801 2963 3467 4140 4704
Size: 113 368 1153 1167 162 504 673 564
Fragments arranged by size:
1167 1153 673 564 504 368 162 113
BbeI G_GCGC'C
Cuts at: 0 25 283 398 666 4704
Size: 25 258 115 268 4038
Fragments arranged by size:
4038 268 258 115 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 1924 1941 1974 3563 4306 4704
Size: 1924 17 33 1589 743 398
Fragments arranged by size:
1924 1589 743 398 33 17
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3354 3388 4704
Size: 3354 34 1316
Fragments arranged by size:
3354 1316 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1855 2212 4704
Size: 1855 357 2492
Fragments arranged by size:
2492 1855 357
BfrBI ATG'CAT
Cuts at: 0 3123 4354 4704
Size: 3123 1231 350
Fragments arranged by size:
3123 1231 350
BglI GCCn_nnn'nGGC
Cuts at: 0 117 148 4704
Size: 117 31 4556
Fragments arranged by size:
4556 117 31
BglII A'GATC_T
Cuts at: 0 2700 4704
Size: 2700 2004
BlpI GC'TnA_GC
Cuts at: 0 2990 4704
Size: 2990 1714
Bme1580I G_kGCm'C
Cuts at: 0 1444 1519 3790 4704
Size: 1444 75 2271 914
Fragments arranged by size:
2271 1444 914 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 2426 4704
Size: 2426 2278
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 288 978 4704
Size: 288 690 3726
Fragments arranged by size:
3726 690 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 873 962 1202 1523 2815 4704
Size: 235 638 89 240 321 1292 1889
Fragments arranged by size:
1889 1292 638 321 240 235 89
Bpu10I CC'TnA_GC
Cuts at: 0 424 557 4704
Size: 424 133 4147
Fragments arranged by size:
4147 424 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 652 3793 4704
Size: 604 48 3141 911
Fragments arranged by size:
3141 911 604 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 12 276 4704
Size: 12 264 4428
Fragments arranged by size:
4428 264 12
BsaAI yAC'GTr
Cuts at: 0 549 2977 4704
Size: 549 2428 1727
Fragments arranged by size:
2428 1727 549
BsaBI GATnn'nnATC
Cuts at: 0 2241 4704
Size: 2241 2463
BsaHI Gr'CG_yC
Cuts at: 0 22 280 395 663 707 911 3134 4175
Size: 22 258 115 268 44 204 2223 1041
Cuts at: 4175 4211 4704
Size: 36 493
Fragments arranged by size:
2223 1041 493 268 258 204 115 44
36 22
BsaWI w'CCGG_w
Cuts at: 0 1658 2963 3579 4704
Size: 1658 1305 616 1125
Fragments arranged by size:
1658 1305 1125 616
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1335 1365 1445 1475 3808 3838 4704
Size: 1335 30 80 30 2333 30 866
Fragments arranged by size:
2333 1335 866 80 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 871 876 1356 1943 3397 4704
Size: 871 5 480 587 1454 1307
Fragments arranged by size:
1454 1307 871 587 480 5
BseYI C'CCAG_C
Cuts at: 0 657 784 1031 1327 1794 2784 4704
Size: 657 127 247 296 467 990 1920
Fragments arranged by size:
1920 990 657 467 296 247 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 679 1650 3659 4704
Size: 243 436 971 2009 1045
Fragments arranged by size:
2009 1045 971 436 243
BsiEI CG_ry'CG
Cuts at: 0 96 124 350 390 488 870 938 1049
Size: 96 28 226 40 98 382 68 111
Cuts at: 1049 2885 4704
Size: 1836 1819
Fragments arranged by size:
1836 1819 382 226 111 98 96 68
40 28
BsiHKAI G_wGCw'C
Cuts at: 0 221 811 1444 1634 2801 2852 2963 4140
Size: 221 590 633 190 1167 51 111 1177
Cuts at: 4140 4704
Size: 564
Fragments arranged by size:
1177 1167 633 590 564 221 190 111
51
BsmI GAATG_Cn'
Cuts at: 0 1795 3718 3985 4342 4704
Size: 1795 1923 267 357 362
Fragments arranged by size:
1923 1795 362 357 267
BsmBI CGTCTCn'nnnn_
Cuts at: 0 559 3125 3372 4704
Size: 559 2566 247 1332
Fragments arranged by size:
2566 1332 559 247
Bsp1286I G_dGCh'C
Cuts at: 0 113 221 481 811 1444 1519 1634 2801
Size: 113 108 260 330 633 75 115 1167
Cuts at: 2801 2852 2963 3467 3790 4140 4704
Size: 51 111 504 323 350 564
Fragments arranged by size:
1167 633 564 504 350 330 323 260
115 113 111 108 75 51
BspEI T'CCGG_A
Cuts at: 0 2963 3579 4704
Size: 2963 616 1125
Fragments arranged by size:
2963 1125 616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1672 3564 4704
Size: 1672 1892 1140
Fragments arranged by size:
1892 1672 1140
BsrBI CCG'CTC
Cuts at: 0 182 764 957 4704
Size: 182 582 193 3747
Fragments arranged by size:
3747 582 193 182
BsrDI GCAATG_nn'
Cuts at: 0 2249 2750 3275 4704
Size: 2249 501 525 1429
Fragments arranged by size:
2249 1429 525 501
BsrFI r'CCGG_y
Cuts at: 0 267 349 373 487 775 4704
Size: 267 82 24 114 288 3929
Fragments arranged by size:
3929 288 267 114 82 24
BsrGI T'GTAC_A
Cuts at: 0 1461 3542 4704
Size: 1461 2081 1162
Fragments arranged by size:
2081 1461 1162
BssHII G'CGCG_C
Cuts at: 0 793 1012 1014 4704
Size: 793 219 2 3690
Fragments arranged by size:
3690 793 219 2
BstBI TT'CG_AA
Cuts at: 0 1536 4704
Size: 1536 3168
BstEII G'GTnAC_C
Cuts at: 0 2892 4704
Size: 2892 1812
BstXI CCAn_nnnn'nTGG
Cuts at: 0 897 2212 4704
Size: 897 1315 2492
Fragments arranged by size:
2492 1315 897
BstYI r'GATC_y
Cuts at: 0 1004 2236 2700 3889 4203 4704
Size: 1004 1232 464 1189 314 501
Fragments arranged by size:
1232 1189 1004 501 464 314
BtgI C'CryG_G
Cuts at: 0 587 852 974 1145 2646 2885 3515 4704
Size: 587 265 122 171 1501 239 630 1189
Fragments arranged by size:
1501 1189 630 587 265 239 171 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 129 597 783 4704
Size: 129 468 186 3921
Fragments arranged by size:
3921 468 186 129
BtsI GCAGTG_nn'
Cuts at: 0 52 1056 1410 3280 3954 4704
Size: 52 1004 354 1870 674 750
Fragments arranged by size:
1870 1004 750 674 354 52
DraI TTT'AAA
Cuts at: 0 2438 4704
Size: 2438 2266
DraIII CAC_nnn'GTG
Cuts at: 0 702 4704
Size: 702 4002
EaeI y'GGCC_r
Cuts at: 0 93 347 485 536 608 644 849 855
Size: 93 254 138 51 72 36 205 6
Cuts at: 855 867 935 1046 1188 2882 3690 3867 4704
Size: 12 68 111 142 1694 808 177 837
Fragments arranged by size:
1694 837 808 254 205 177 142 138
111 93 72 68 51 36 12 6
EagI C'GGCC_G
Cuts at: 0 93 347 485 867 935 1046 2882 4704
Size: 93 254 138 382 68 111 1836 1822
Fragments arranged by size:
1836 1822 382 254 138 111 93 68
EarI CTCTTCn'nnn_
Cuts at: 0 994 1256 2171 2232 2647 2766 4250 4704
Size: 994 262 915 61 415 119 1484 454
Fragments arranged by size:
1484 994 915 454 415 262 119 61
EcoICRI GAG'CTC
Cuts at: 0 1632 2799 2961 4138 4704
Size: 1632 1167 162 1177 566
Fragments arranged by size:
1632 1177 1167 566 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 297 873 962 975 1202 1523 2815
Size: 235 62 576 89 13 227 321 1292
Cuts at: 2815 3134 3736 3914 4288 4704
Size: 319 602 178 374 416
Fragments arranged by size:
1292 602 576 416 374 321 319 235
227 178 89 62 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 898 3086 4704
Size: 898 2188 1618
Fragments arranged by size:
2188 1618 898
EcoO109I rG'GnC_Cy
Cuts at: 0 903 2526 3511 4704
Size: 903 1623 985 1193
Fragments arranged by size:
1623 1193 985 903
EcoRI G'AATT_C
Cuts at: 0 1346 3583 4704
Size: 1346 2237 1121
Fragments arranged by size:
2237 1346 1121
EcoRV GAT'ATC
Cuts at: 0 3881 4422 4704
Size: 3881 541 282
Fragments arranged by size:
3881 541 282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1460 4704
Size: 1460 3244
FseI GG_CCGG'CC
Cuts at: 0 353 4704
Size: 353 4351
FspI TGC'GCA
Cuts at: 0 442 2455 4704
Size: 442 2013 2249
Fragments arranged by size:
2249 2013 442
FspAI rTGC'GCAy
Cuts at: 0 2455 4704
Size: 2455 2249
HaeII r_GCGC'y
Cuts at: 0 25 52 283 398 666 770 963 982
Size: 25 27 231 115 268 104 193 19
Cuts at: 982 4704
Size: 3722
Fragments arranged by size:
3722 268 231 193 115 104 27 25
19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 1114 1146 1483 1515 1906 1938
Size: 445 32 637 32 337 32 391 32
Cuts at: 1938 2800 2832 3808 3840 4704
Size: 862 32 976 32 864
Fragments arranged by size:
976 864 862 637 445 391 337 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1716 2935 4067 4704
Size: 1716 1219 1132 637
Fragments arranged by size:
1716 1219 1132 637
HindIII A'AGCT_T
Cuts at: 0 1575 3189 4402 4704
Size: 1575 1614 1213 302
Fragments arranged by size:
1614 1575 1213 302
KasI G'GCGC_C
Cuts at: 0 21 279 394 662 4704
Size: 21 258 115 268 4042
Fragments arranged by size:
4042 268 258 115 21
KpnI G_GTAC'C
Cuts at: 0 528 4704
Size: 528 4176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 620 1113 1145 3438 3631 4704
Size: 104 516 493 32 2293 193 1073
Fragments arranged by size:
2293 1073 516 493 193 104 32
MscI TGG'CCA
Cuts at: 0 646 1190 3692 3869 4704
Size: 646 544 2502 177 835
Fragments arranged by size:
2502 835 646 544 177
MslI CAynn'nnrTG
Cuts at: 0 454 547 814 2080 2152 4535 4704
Size: 454 93 267 1266 72 2383 169
Fragments arranged by size:
2383 1266 454 267 169 93 72
MspA1I CmG'CkG
Cuts at: 0 188 201 521 578 589 940 1031 1327
Size: 188 13 320 57 11 351 91 296
Cuts at: 1327 4704
Size: 3377
Fragments arranged by size:
3377 351 320 296 188 91 57 13
11
NaeI GCC'GGC
Cuts at: 0 351 489 4704
Size: 351 138 4215
Fragments arranged by size:
4215 351 138
NarI GG'CG_CC
Cuts at: 0 22 280 395 663 4704
Size: 22 258 115 268 4041
Fragments arranged by size:
4041 268 258 115 22
NcoI C'CATG_G
Cuts at: 0 2646 4704
Size: 2646 2058
NgoMIV G'CCGG_C
Cuts at: 0 349 487 4704
Size: 349 138 4217
Fragments arranged by size:
4217 349 138
NotI GC'GGCC_GC
Cuts at: 0 935 4704
Size: 935 3769
NsiI A_TGCA'T
Cuts at: 0 3125 4356 4704
Size: 3125 1231 348
Fragments arranged by size:
3125 1231 348
NspI r_CATG'y
Cuts at: 0 1802 3360 4663 4704
Size: 1802 1558 1303 41
Fragments arranged by size:
1802 1558 1303 41
PasI CC'CwG_GG
Cuts at: 0 414 4704
Size: 414 4290
PflMI CCAn_nnn'nTGG
Cuts at: 0 779 4704
Size: 779 3925
PfoI T'CCnGG_A
Cuts at: 0 10 3446 3623 4704
Size: 10 3436 177 1081
Fragments arranged by size:
3436 1081 177 10
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 805 837 4704
Size: 805 32 3867
Fragments arranged by size:
3867 805 32
PpuMI rG'GwC_Cy
Cuts at: 0 2526 3511 4704
Size: 2526 985 1193
Fragments arranged by size:
2526 1193 985
PshAI GACnn'nnGTC
Cuts at: 0 3251 4704
Size: 3251 1453
PstI C_TGCA'G
Cuts at: 0 2516 2999 4704
Size: 2516 483 1705
Fragments arranged by size:
2516 1705 483
PvuII CAG'CTG
Cuts at: 0 188 201 1031 1327 4704
Size: 188 13 830 296 3377
Fragments arranged by size:
3377 830 296 188 13
SacI G_AGCT'C
Cuts at: 0 1634 2801 2963 4140 4704
Size: 1634 1167 162 1177 564
Fragments arranged by size:
1634 1177 1167 564 162
SacII CC_GC'GG
Cuts at: 0 590 4704
Size: 590 4114
SalI G'TCGA_C
Cuts at: 0 4065 4704
Size: 4065 639
SanDI GG'GwC_CC
Cuts at: 0 3511 4704
Size: 3511 1193
SapI GCTCTTCn'nnn_
Cuts at: 0 2232 4704
Size: 2232 2472
ScaI AGT'ACT
Cuts at: 0 3591 3943 4704
Size: 3591 352 761
Fragments arranged by size:
3591 761 352
SfcI C'TryA_G
Cuts at: 0 207 1556 1671 2512 2995 4704
Size: 207 1349 115 841 483 1709
Fragments arranged by size:
1709 1349 841 483 207 115
SfoI GGC'GCC
Cuts at: 0 23 281 396 664 4704
Size: 23 258 115 268 4040
Fragments arranged by size:
4040 268 258 115 23
SmaI CCC'GGG
Cuts at: 0 303 2065 4704
Size: 303 1762 2639
Fragments arranged by size:
2639 1762 303
SmlI C'TyrA_G
Cuts at: 0 619 667 1198 3723 3772 4704
Size: 619 48 531 2525 49 932
Fragments arranged by size:
2525 932 619 531 49 48
SphI G_CATG'C
Cuts at: 0 1802 4704
Size: 1802 2902
SspI AAT'ATT
Cuts at: 0 2398 2613 2685 3657 4591 4704
Size: 2398 215 72 972 934 113
Fragments arranged by size:
2398 972 934 215 113 72
StyI C'CwwG_G
Cuts at: 0 1940 2646 4070 4415 4704
Size: 1940 706 1424 345 289
Fragments arranged by size:
1940 1424 706 345 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2164 3115 4704
Size: 2164 951 1589
Fragments arranged by size:
2164 1589 951
TatI w'GTAC_w
Cuts at: 0 1461 2481 3542 3589 3941 4704
Size: 1461 1020 1061 47 352 763
Fragments arranged by size:
1461 1061 1020 763 352 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1335 1367 2286 2318 4704
Size: 1335 32 919 32 2386
Fragments arranged by size:
2386 1335 919 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 654 2212 2653 3336 4704
Size: 654 1558 441 683 1368
Fragments arranged by size:
1558 1368 683 654 441
XmaI C'CCGG_G
Cuts at: 0 301 2063 4704
Size: 301 1762 2641
Fragments arranged by size:
2641 1762 301
ZraI GAC'GTC
Cuts at: 0 912 4212 4704
Size: 912 3300 492
Fragments arranged by size:
3300 912 492
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AleI AlfI AlwNI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NsiI NspI
PasI PflMI PfoI PpiI PpuMI PshAI PstI PvuII
SacI SacII SalI SanDI SapI ScaI SfcI SfoI
SmaI SmlI SphI SspI StyI TaqII TatI TstI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflIII AgeI AhdI AloI ApaI
AscI AseI AsiSI AvrII BaeI BbvCI BclI BmgBI
BmtI BsiWI BspHI BssSI BstAPI BstZ17I Bsu36I ClaI
CspCI DrdI EciI HpaI MfeI MluI NdeI NheI
NruI PacI PciI PmeI PmlI PsiI PspOMI PspXI
PsrI PvuI RsrII SbfI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI StuI SwaI Tth111I XbaI XhoI
XmnI