Restriction map for
pF1KE0052
(Linear) (Six-base) MAPSORT of: KIEE0052.seq Check: 8537 from: 1 to: 3261
KIEE0052.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 0 2310 2735 3261
Size: 2310 425 526
Fragments arranged by size:
2310 526 425
AclI AA'CG_TT
Cuts at: 0 2486 3261
Size: 2486 775
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 922 977 1024 1523 3178 3261
Size: 16 906 55 47 499 1655 83
Fragments arranged by size:
1655 906 499 83 55 47 16
AflII C'TTAA_G
Cuts at: 0 1921 3261
Size: 1921 1340
AflIII A'CryG_T
Cuts at: 0 217 3261
Size: 217 3044
AleI CACnn'nnGTG
Cuts at: 0 753 2743 3261
Size: 753 1990 518
Fragments arranged by size:
1990 753 518
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 550 582 3261
Size: 550 32 2679
Fragments arranged by size:
2679 550 32
ApoI r'AATT_y
Cuts at: 0 427 655 1185 1738 1812 2023 2336 3152
Size: 427 228 530 553 74 211 313 816
Cuts at: 3152 3198 3261
Size: 46 63
Fragments arranged by size:
816 553 530 427 313 228 211 74
63 46
AseI AT'TA_AT
Cuts at: 0 883 1307 2255 2570 3261
Size: 883 424 948 315 691
Fragments arranged by size:
948 883 691 424 315
AvaI C'yCGr_G
Cuts at: 0 172 3261
Size: 172 3089
BanI G'GyrC_C
Cuts at: 0 176 3261
Size: 176 3085
BanII G_rGCy'C
Cuts at: 0 140 2914 3261
Size: 140 2774 347
Fragments arranged by size:
2774 347 140
BbsI GAAGACnn'nnnn_
Cuts at: 0 580 1044 1447 1648 2435 3176 3261
Size: 580 464 403 201 787 741 85
Fragments arranged by size:
787 741 580 464 403 201 85
BfrBI ATG'CAT
Cuts at: 0 880 1201 2356 2641 3261
Size: 880 321 1155 285 620
Fragments arranged by size:
1155 880 620 321 285
BglII A'GATC_T
Cuts at: 0 1905 2557 3261
Size: 1905 652 704
Fragments arranged by size:
1905 704 652
Bme1580I G_kGCm'C
Cuts at: 0 929 3261
Size: 929 2332
BmgBI CAC'GTC
Cuts at: 0 96 3261
Size: 96 3165
BmrI ACTGGGnnnn_n'
Cuts at: 0 1871 3261
Size: 1871 1390
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 608 3261
Size: 608 2653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 835 2867 2936 3018 3261
Size: 393 442 2032 69 82 243
Fragments arranged by size:
2032 442 393 243 82 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1989 3261
Size: 1989 1272
BsaI GGTCTCn'nnnn_
Cuts at: 0 411 3261
Size: 411 2850
BsaBI GATnn'nnATC
Cuts at: 0 1340 1367 3261
Size: 1340 27 1894
Fragments arranged by size:
1894 1340 27
BsaHI Gr'CG_yC
Cuts at: 0 128 2010 3261
Size: 128 1882 1251
Fragments arranged by size:
1882 1251 128
BsaWI w'CCGG_w
Cuts at: 0 2795 3261
Size: 2795 466
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 29 59 3261
Size: 29 30 3202
Fragments arranged by size:
3202 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 115 148 2692 2797 2856 3261
Size: 82 33 33 2544 105 59 405
Fragments arranged by size:
2544 405 105 82 59 33 33
BseYI C'CCAG_C
Cuts at: 0 2932 3261
Size: 2932 329
BsiHKAI G_wGCw'C
Cuts at: 0 140 384 1829 2914 3261
Size: 140 244 1445 1085 347
Fragments arranged by size:
1445 1085 347 244 140
BsmI GAATG_Cn'
Cuts at: 0 882 1539 2358 2805 3261
Size: 882 657 819 447 456
Fragments arranged by size:
882 819 657 456 447
Bsp1286I G_dGCh'C
Cuts at: 0 140 384 929 1829 2914 3261
Size: 140 244 545 900 1085 347
Fragments arranged by size:
1085 900 545 347 244 140
BspEI T'CCGG_A
Cuts at: 0 2795 3261
Size: 2795 466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 29 3261
Size: 29 3232
BsrDI GCAATG_nn'
Cuts at: 0 2505 3261
Size: 2505 756
BstEII G'GTnAC_C
Cuts at: 0 1273 3261
Size: 1273 1988
BstXI CCAn_nnnn'nTGG
Cuts at: 0 60 3261
Size: 60 3201
BstYI r'GATC_y
Cuts at: 0 57 1905 2557 3261
Size: 57 1848 652 704
Fragments arranged by size:
1848 704 652 57
BtgI C'CryG_G
Cuts at: 0 754 1732 2902 3261
Size: 754 978 1170 359
Fragments arranged by size:
1170 978 754 359
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 819 1071 1208 3261
Size: 819 252 137 2053
Fragments arranged by size:
2053 819 252 137
BtsI GCAGTG_nn'
Cuts at: 0 150 3261
Size: 150 3111
DraI TTT'AAA
Cuts at: 0 687 3261
Size: 687 2574
DraIII CAC_nnn'GTG
Cuts at: 0 1673 3261
Size: 1673 1588
DrdI GACnn_nn'nnGTC
Cuts at: 0 1923 3261
Size: 1923 1338
EaeI y'GGCC_r
Cuts at: 0 2 3261
Size: 2 3259
EarI CTCTTCn'nnn_
Cuts at: 0 104 554 728 851 2105 2902 3015 3261
Size: 104 450 174 123 1254 797 113 246
Fragments arranged by size:
1254 797 450 246 174 123 113 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1597 3261
Size: 1597 1664
EcoICRI GAG'CTC
Cuts at: 0 138 2912 3261
Size: 138 2774 349
Fragments arranged by size:
2774 349 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 393 835 922 977 1024 1523 2867
Size: 16 377 442 87 55 47 499 1344
Cuts at: 2867 2936 3018 3178 3261
Size: 69 82 160 83
Fragments arranged by size:
1344 499 442 377 160 87 83 82
69 55 47 16
EcoO109I rG'GnC_Cy
Cuts at: 0 37 2929 3261
Size: 37 2892 332
Fragments arranged by size:
2892 332 37
EcoRI G'AATT_C
Cuts at: 0 655 1185 3261
Size: 655 530 2076
Fragments arranged by size:
2076 655 530
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2490 3261
Size: 2490 771
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 61 97 129 2881 2913 3261
Size: 29 32 36 32 2752 32 348
Fragments arranged by size:
2752 348 36 32 32 32 29
HincII GTy'rAC
Cuts at: 0 1573 3261
Size: 1573 1688
HindIII A'AGCT_T
Cuts at: 0 1843 3261
Size: 1843 1418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1463 2080 3261
Size: 799 664 617 1181
Fragments arranged by size:
1181 799 664 617
MscI TGG'CCA
Cuts at: 0 4 3261
Size: 4 3257
MslI CAynn'nnrTG
Cuts at: 0 377 753 932 1133 2743 3261
Size: 377 376 179 201 1610 518
Fragments arranged by size:
1610 518 377 376 201 179
MspA1I CmG'CkG
Cuts at: 0 855 1332 1630 2759 3261
Size: 855 477 298 1129 502
Fragments arranged by size:
1129 855 502 477 298
NcoI C'CATG_G
Cuts at: 0 1732 3261
Size: 1732 1529
NdeI CA'TA_TG
Cuts at: 0 933 974 1035 3261
Size: 933 41 61 2226
Fragments arranged by size:
2226 933 61 41
NruI TCG'CGA
Cuts at: 0 3102 3261
Size: 3102 159
NsiI A_TGCA'T
Cuts at: 0 882 1203 2358 2643 3261
Size: 882 321 1155 285 618
Fragments arranged by size:
1155 882 618 321 285
NspI r_CATG'y
Cuts at: 0 221 2641 2734 3261
Size: 221 2420 93 527
Fragments arranged by size:
2420 527 221 93
PasI CC'CwG_GG
Cuts at: 0 53 3261
Size: 53 3208
PciI A'CATG_T
Cuts at: 0 217 3261
Size: 217 3044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 3261
Size: 103 32 3126
Fragments arranged by size:
3126 103 32
PpuMI rG'GwC_Cy
Cuts at: 0 37 2929 3261
Size: 37 2892 332
Fragments arranged by size:
2892 332 37
PstI C_TGCA'G
Cuts at: 0 145 2169 3261
Size: 145 2024 1092
Fragments arranged by size:
2024 1092 145
PvuII CAG'CTG
Cuts at: 0 855 1332 1630 3261
Size: 855 477 298 1631
Fragments arranged by size:
1631 855 477 298
SacI G_AGCT'C
Cuts at: 0 140 2914 3261
Size: 140 2774 347
Fragments arranged by size:
2774 347 140
SapI GCTCTTCn'nnn_
Cuts at: 0 2902 3261
Size: 2902 359
SexAI A'CCwGG_T
Cuts at: 0 1887 3261
Size: 1887 1374
SfcI C'TryA_G
Cuts at: 0 141 783 1592 1616 1787 2165 2960 3261
Size: 141 642 809 24 171 378 795 301
Fragments arranged by size:
809 795 642 378 301 171 141 24
SmaI CCC'GGG
Cuts at: 0 174 3261
Size: 174 3087
SmlI C'TyrA_G
Cuts at: 0 1921 2004 3261
Size: 1921 83 1257
Fragments arranged by size:
1921 1257 83
SspI AAT'ATT
Cuts at: 0 2623 3261
Size: 2623 638
StuI AGG'CCT
Cuts at: 0 3007 3261
Size: 3007 254
StyI C'CwwG_G
Cuts at: 0 1123 1732 1854 3261
Size: 1123 609 122 1407
Fragments arranged by size:
1407 1123 609 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 66 1794 3261
Size: 66 1728 1467
Fragments arranged by size:
1728 1467 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1278 1310 3261
Size: 1278 32 1951
Fragments arranged by size:
1951 1278 32
Tth111I GACn'n_nGTC
Cuts at: 0 963 3261
Size: 963 2298
XbaI T'CTAG_A
Cuts at: 0 2412 3261
Size: 2412 849
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 514 2574 3261
Size: 60 454 2060 687
Fragments arranged by size:
2060 687 454 60
XmaI C'CCGG_G
Cuts at: 0 172 3261
Size: 172 3089
XmnI GAAnn'nnTTC
Cuts at: 0 659 2845 3261
Size: 659 2186 416
Fragments arranged by size:
2186 659 416
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AleI AloI ApoI
AseI AvaI BanI BanII BbsI BfrBI BglII Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrDI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII HindIII MmeI MscI
MslI MspA1I NcoI NdeI NruI NsiI NspI PasI
PciI PpiI PpuMI PstI PvuII SacI SapI SexAI
SfcI SmaI SmlI SspI StuI StyI TaqII TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AhdI AlfI AlwNI
ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BclI BglI BlpI BmtI Bpu10I
BsaAI BsgI BsiEI BsiWI BsmBI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I ClaI
CspCI EagI EcoNI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV
NheI NotI PacI PflMI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SwaI TatI XhoI ZraI