Restriction map for
pF1KE0049
(Linear) (Six-base) MAPSORT of: KIEE0049.seq Check: 7642 from: 1 to: 2181
KIEE0049.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 2181
Size: 37 2144
AflIII A'CryG_T
Cuts at: 0 1995 2181
Size: 1995 186
AleI CACnn'nnGTG
Cuts at: 0 650 1626 1628 2181
Size: 650 976 2 553
Fragments arranged by size:
976 650 553 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 668 2181
Size: 668 1513
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 935 967 2181
Size: 935 32 1214
Fragments arranged by size:
1214 935 32
AlwNI CAG_nnn'CTG
Cuts at: 0 964 2181
Size: 964 1217
ApoI r'AATT_y
Cuts at: 0 595 1289 1972 2104 2147 2181
Size: 595 694 683 132 43 34
Fragments arranged by size:
694 683 595 132 43 34
BamHI G'GATC_C
Cuts at: 0 2169 2181
Size: 2169 12
BanI G'GyrC_C
Cuts at: 0 652 2181
Size: 652 1529
BanII G_rGCy'C
Cuts at: 0 2014 2181
Size: 2014 167
BbsI GAAGACnn'nnnn_
Cuts at: 0 2099 2181
Size: 2099 82
BbvCI CC'TCA_GC
Cuts at: 0 878 2181
Size: 878 1303
BclI T'GATC_A
Cuts at: 0 567 2181
Size: 567 1614
Bme1580I G_kGCm'C
Cuts at: 0 1320 2181
Size: 1320 861
BmrI ACTGGGnnnn_n'
Cuts at: 0 185 535 1858 2181
Size: 185 350 1323 323
Fragments arranged by size:
1323 350 323 185
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 689 1281 1356 2181
Size: 650 39 592 75 825
Fragments arranged by size:
825 650 592 75 39
Bpu10I CC'TnA_GC
Cuts at: 0 746 878 2181
Size: 746 132 1303
Fragments arranged by size:
1303 746 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 2181
Size: 593 1588
BsaI GGTCTCn'nnnn_
Cuts at: 0 1259 2083 2181
Size: 1259 824 98
Fragments arranged by size:
1259 824 98
BsaAI yAC'GTr
Cuts at: 0 1321 2181
Size: 1321 860
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 935 965 2181
Size: 935 30 1216
Fragments arranged by size:
1216 935 30
BseYI C'CCAG_C
Cuts at: 0 1234 2181
Size: 1234 947
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 2181
Size: 46 2135
BsiHKAI G_wGCw'C
Cuts at: 0 173 747 1862 2181
Size: 173 574 1115 319
Fragments arranged by size:
1115 574 319 173
BsmI GAATG_Cn'
Cuts at: 0 1734 2181
Size: 1734 447
Bsp1286I G_dGCh'C
Cuts at: 0 173 747 1320 1862 2014 2181
Size: 173 574 573 542 152 167
Fragments arranged by size:
574 573 542 173 167 152
BsrDI GCAATG_nn'
Cuts at: 0 320 734 1743 2181
Size: 320 414 1009 438
Fragments arranged by size:
1009 438 414 320
BssSI C'ACGA_G
Cuts at: 0 1624 2181
Size: 1624 557
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1015 2181
Size: 1015 1166
BstBI TT'CG_AA
Cuts at: 0 600 2181
Size: 600 1581
BstXI CCAn_nnnn'nTGG
Cuts at: 0 315 2181
Size: 315 1866
BstYI r'GATC_y
Cuts at: 0 513 1047 1374 2169 2181
Size: 513 534 327 795 12
Fragments arranged by size:
795 534 513 327 12
BtgI C'CryG_G
Cuts at: 0 229 2181
Size: 229 1952
BtsI GCAGTG_nn'
Cuts at: 0 626 965 2181
Size: 626 339 1216
Fragments arranged by size:
1216 626 339
DraI TTT'AAA
Cuts at: 0 1977 2181
Size: 1977 204
EaeI y'GGCC_r
Cuts at: 0 157 305 1443 2181
Size: 157 148 1138 738
Fragments arranged by size:
1138 738 157 148
EarI CTCTTCn'nnn_
Cuts at: 0 75 1095 1456 1850 2181
Size: 75 1020 361 394 331
Fragments arranged by size:
1020 394 361 331 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 650 689 1281 1356 2181
Size: 37 613 39 592 75 825
Fragments arranged by size:
825 613 592 75 39 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1277 2181
Size: 1277 904
EcoO109I rG'GnC_Cy
Cuts at: 0 503 536 2181
Size: 503 33 1645
Fragments arranged by size:
1645 503 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1154 1309 2181
Size: 1154 155 872
Fragments arranged by size:
1154 872 155
HincII GTy'rAC
Cuts at: 0 1332 1683 2181
Size: 1332 351 498
Fragments arranged by size:
1332 498 351
HindIII A'AGCT_T
Cuts at: 0 344 2181
Size: 344 1837
HpaI GTT'AAC
Cuts at: 0 1683 2181
Size: 1683 498
MfeI C'AATT_G
Cuts at: 0 1217 2051 2181
Size: 1217 834 130
Fragments arranged by size:
1217 834 130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 1770 2181
Size: 2 1768 411
Fragments arranged by size:
1768 411 2
MscI TGG'CCA
Cuts at: 0 159 307 1445 2181
Size: 159 148 1138 736
Fragments arranged by size:
1138 736 159 148
MslI CAynn'nnrTG
Cuts at: 0 398 650 1243 1626 1628 2039 2181
Size: 398 252 593 383 2 411 142
Fragments arranged by size:
593 411 398 383 252 142 2
MspA1I CmG'CkG
Cuts at: 0 1756 2181
Size: 1756 425
NcoI C'CATG_G
Cuts at: 0 229 2181
Size: 229 1952
NspI r_CATG'y
Cuts at: 0 715 826 1242 1999 2181
Size: 715 111 416 757 182
Fragments arranged by size:
757 715 416 182 111
PciI A'CATG_T
Cuts at: 0 1995 2181
Size: 1995 186
PfoI T'CCnGG_A
Cuts at: 0 705 2172 2181
Size: 705 1467 9
Fragments arranged by size:
1467 705 9
PmlI CAC'GTG
Cuts at: 0 1321 2181
Size: 1321 860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 935 967 2181
Size: 935 32 1214
Fragments arranged by size:
1214 935 32
PpuMI rG'GwC_Cy
Cuts at: 0 536 2181
Size: 536 1645
PstI C_TGCA'G
Cuts at: 0 783 1281 1939 2181
Size: 783 498 658 242
Fragments arranged by size:
783 658 498 242
PvuII CAG'CTG
Cuts at: 0 1756 2181
Size: 1756 425
SapI GCTCTTCn'nnn_
Cuts at: 0 75 1850 2181
Size: 75 1775 331
Fragments arranged by size:
1775 331 75
SfcI C'TryA_G
Cuts at: 0 779 806 1115 1277 1935 2181
Size: 779 27 309 162 658 246
Fragments arranged by size:
779 658 309 246 162 27
SmlI C'TyrA_G
Cuts at: 0 608 2181
Size: 608 1573
SphI G_CATG'C
Cuts at: 0 826 1242 2181
Size: 826 416 939
Fragments arranged by size:
939 826 416
StuI AGG'CCT
Cuts at: 0 1228 2181
Size: 1228 953
StyI C'CwwG_G
Cuts at: 0 229 532 1312 2181
Size: 229 303 780 869
Fragments arranged by size:
869 780 303 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1648 1877 2181
Size: 1648 229 304
Fragments arranged by size:
1648 304 229
TatI w'GTAC_w
Cuts at: 0 1542 1644 2181
Size: 1542 102 537
Fragments arranged by size:
1542 537 102
Tth111I GACn'n_nGTC
Cuts at: 0 472 760 2181
Size: 472 288 1421
Fragments arranged by size:
1421 472 288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1059 1242 2181
Size: 1059 183 939
Fragments arranged by size:
1059 939 183
XmnI GAAnn'nnTTC
Cuts at: 0 815 1289 2181
Size: 815 474 892
Fragments arranged by size:
892 815 474
Enzymes that do cut:
AcuI AflIII AleI AlfI AloI AlwNI ApoI BamHI
BanI BanII BbsI BbvCI BclI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaXI BseYI BsgI BsiHKAI
BsmI Bsp1286I BsrDI BssSI BstAPI BstBI BstXI BstYI
BtgI BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I
FalI HincII HindIII HpaI MfeI MmeI MscI MslI
MspA1I NcoI NspI PciI PfoI PmlI PpiI PpuMI
PstI PvuII SapI SfcI SmlI SphI StuI StyI
TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BsaBI BsaHI BsaWI BseRI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI HaeII Hin4I KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TstI XbaI XhoI XmaI ZraI