Restriction map for
pF1KE0047
(Linear) (Six-base) MAPSORT of: KIEE0047.seq Check: 3356 from: 1 to: 2259
KIEE0047.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1155 2259
Size: 1155 1104
Acc65I G'GTAC_C
Cuts at: 0 86 327 2259
Size: 86 241 1932
Fragments arranged by size:
1932 241 86
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 1295 1496 1694 1906 2259
Size: 180 1115 201 198 212 353
Fragments arranged by size:
1115 353 212 201 198 180
AlwNI CAG_nnn'CTG
Cuts at: 0 1273 2237 2259
Size: 1273 964 22
Fragments arranged by size:
1273 964 22
ApaLI G'TGCA_C
Cuts at: 0 2252 2259
Size: 2252 7
ApoI r'AATT_y
Cuts at: 0 1486 1726 2259
Size: 1486 240 533
Fragments arranged by size:
1486 533 240
AvrII C'CTAG_G
Cuts at: 0 1806 2259
Size: 1806 453
BanI G'GyrC_C
Cuts at: 0 10 86 327 1199 2259
Size: 10 76 241 872 1060
Fragments arranged by size:
1060 872 241 76 10
BanII G_rGCy'C
Cuts at: 0 1015 1157 1177 1247 1683 2259
Size: 1015 142 20 70 436 576
Fragments arranged by size:
1015 576 436 142 70 20
BbvCI CC'TCA_GC
Cuts at: 0 1828 2259
Size: 1828 431
BclI T'GATC_A
Cuts at: 0 1998 2259
Size: 1998 261
BglI GCCn_nnn'nGGC
Cuts at: 0 61 1077 2189 2259
Size: 61 1016 1112 70
Fragments arranged by size:
1112 1016 70 61
BglII A'GATC_T
Cuts at: 0 1713 2259
Size: 1713 546
BlpI GC'TnA_GC
Cuts at: 0 1254 1851 2259
Size: 1254 597 408
Fragments arranged by size:
1254 597 408
Bme1580I G_kGCm'C
Cuts at: 0 2256 2259
Size: 2256 3
BmrI ACTGGGnnnn_n'
Cuts at: 0 1582 1785 2259
Size: 1582 203 474
Fragments arranged by size:
1582 474 203
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1263 2259
Size: 1263 996
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 153 922 1101 1207 1888 2173 2259
Size: 130 23 769 179 106 681 285 86
Fragments arranged by size:
769 681 285 179 130 106 86 23
Bpu10I CC'TnA_GC
Cuts at: 0 1828 2259
Size: 1828 431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1337 1788 2259
Size: 1337 451 471
Fragments arranged by size:
1337 471 451
BsaI GGTCTCn'nnnn_
Cuts at: 0 501 1966 2259
Size: 501 1465 293
Fragments arranged by size:
1465 501 293
BsaAI yAC'GTr
Cuts at: 0 99 2259
Size: 99 2160
BsaWI w'CCGG_w
Cuts at: 0 1228 2259
Size: 1228 1031
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 404 434 1681 1711 1783 1813 1861 1891
Size: 404 30 1247 30 72 30 48 30
Cuts at: 1891 2259
Size: 368
Fragments arranged by size:
1247 404 368 72 48 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 529 939 1000 1255 1321 1397 1400 1678
Size: 529 410 61 255 66 76 3 278
Cuts at: 1678 2259
Size: 581
Fragments arranged by size:
581 529 410 278 255 76 66 61
3
BseYI C'CCAG_C
Cuts at: 0 160 223 2259
Size: 160 63 2036
Fragments arranged by size:
2036 160 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1001 1621 2259
Size: 1001 620 638
Fragments arranged by size:
1001 638 620
BsiHKAI G_wGCw'C
Cuts at: 0 1015 1157 1177 1683 2256 2259
Size: 1015 142 20 506 573 3
Fragments arranged by size:
1015 573 506 142 20 3
BsmI GAATG_Cn'
Cuts at: 0 48 400 1471 2209 2259
Size: 48 352 1071 738 50
Fragments arranged by size:
1071 738 352 50 48
Bsp1286I G_dGCh'C
Cuts at: 0 1015 1157 1177 1247 1683 2256 2259
Size: 1015 142 20 70 436 573 3
Fragments arranged by size:
1015 573 436 142 70 20 3
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1100 1155 1308 2259
Size: 1100 55 153 951
Fragments arranged by size:
1100 951 153 55
BsrBI CCG'CTC
Cuts at: 0 1055 1197 1253 2259
Size: 1055 142 56 1006
Fragments arranged by size:
1055 1006 142 56
BsrFI r'CCGG_y
Cuts at: 0 89 1202 2259
Size: 89 1113 1057
Fragments arranged by size:
1113 1057 89
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1127 2259
Size: 1127 1132
BstEII G'GTnAC_C
Cuts at: 0 1224 1620 2259
Size: 1224 396 639
Fragments arranged by size:
1224 639 396
BstYI r'GATC_y
Cuts at: 0 653 1713 2259
Size: 653 1060 546
Fragments arranged by size:
1060 653 546
BtgI C'CryG_G
Cuts at: 0 1033 1178 1346 1816 2259
Size: 1033 145 168 470 443
Fragments arranged by size:
1033 470 443 168 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2207 2259
Size: 2207 52
BtsI GCAGTG_nn'
Cuts at: 0 312 2259
Size: 312 1947
DraIII CAC_nnn'GTG
Cuts at: 0 527 2259
Size: 527 1732
EaeI y'GGCC_r
Cuts at: 0 543 839 1895 2127 2259
Size: 543 296 1056 232 132
Fragments arranged by size:
1056 543 296 232 132
EarI CTCTTCn'nnn_
Cuts at: 0 961 985 990 1003 1064 1071 2259
Size: 961 24 5 13 61 7 1188
Fragments arranged by size:
1188 961 61 24 13 7 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1836 2152 2259
Size: 1836 316 107
Fragments arranged by size:
1836 316 107
EcoICRI GAG'CTC
Cuts at: 0 1013 1155 1175 1681 2259
Size: 1013 142 20 506 578
Fragments arranged by size:
1013 578 506 142 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 153 180 922 1101 1207 1295 1496
Size: 130 23 27 742 179 106 88 201
Cuts at: 1496 1694 1888 1906 2173 2259
Size: 198 194 18 267 86
Fragments arranged by size:
742 267 201 198 194 179 130 106
88 86 27 23 18
EcoO109I rG'GnC_Cy
Cuts at: 0 1024 1112 1559 2259
Size: 1024 88 447 700
Fragments arranged by size:
1024 700 447 88
EcoRI G'AATT_C
Cuts at: 0 1486 2259
Size: 1486 773
EcoRV GAT'ATC
Cuts at: 0 1329 2139 2259
Size: 1329 810 120
Fragments arranged by size:
1329 810 120
HaeII r_GCGC'y
Cuts at: 0 96 2259
Size: 96 2163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 1861 1893 2259
Size: 301 32 1528 32 366
Fragments arranged by size:
1528 366 301 32 32
KpnI G_GTAC'C
Cuts at: 0 90 331 2259
Size: 90 241 1928
Fragments arranged by size:
1928 241 90
MfeI C'AATT_G
Cuts at: 0 547 2259
Size: 547 1712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1553 1988 2259
Size: 1553 435 271
Fragments arranged by size:
1553 435 271
MscI TGG'CCA
Cuts at: 0 545 841 1897 2129 2259
Size: 545 296 1056 232 130
Fragments arranged by size:
1056 545 296 232 130
MslI CAynn'nnrTG
Cuts at: 0 1464 2259
Size: 1464 795
MspA1I CmG'CkG
Cuts at: 0 477 1133 1786 1839 2259
Size: 477 656 653 53 420
Fragments arranged by size:
656 653 477 420 53
NaeI GCC'GGC
Cuts at: 0 1204 2259
Size: 1204 1055
NcoI C'CATG_G
Cuts at: 0 1033 1346 1816 2259
Size: 1033 313 470 443
Fragments arranged by size:
1033 470 443 313
NdeI CA'TA_TG
Cuts at: 0 606 731 2259
Size: 606 125 1528
Fragments arranged by size:
1528 606 125
NgoMIV G'CCGG_C
Cuts at: 0 1202 2259
Size: 1202 1057
NruI TCG'CGA
Cuts at: 0 1916 2259
Size: 1916 343
NspI r_CATG'y
Cuts at: 0 1443 1866 2259
Size: 1443 423 393
Fragments arranged by size:
1443 423 393
PflMI CCAn_nnn'nTGG
Cuts at: 0 1034 2259
Size: 1034 1225
PfoI T'CCnGG_A
Cuts at: 0 2150 2259
Size: 2150 109
PpuMI rG'GwC_Cy
Cuts at: 0 1024 1112 2259
Size: 1024 88 1147
Fragments arranged by size:
1147 1024 88
PsiI TTA'TAA
Cuts at: 0 575 2259
Size: 575 1684
PstI C_TGCA'G
Cuts at: 0 1132 2242 2259
Size: 1132 1110 17
Fragments arranged by size:
1132 1110 17
PvuII CAG'CTG
Cuts at: 0 477 1839 2259
Size: 477 1362 420
Fragments arranged by size:
1362 477 420
SacI G_AGCT'C
Cuts at: 0 1015 1157 1177 1683 2259
Size: 1015 142 20 506 576
Fragments arranged by size:
1015 576 506 142 20
SanDI GG'GwC_CC
Cuts at: 0 1024 2259
Size: 1024 1235
SapI GCTCTTCn'nnn_
Cuts at: 0 990 1003 2259
Size: 990 13 1256
Fragments arranged by size:
1256 990 13
SfcI C'TryA_G
Cuts at: 0 149 1128 2238 2259
Size: 149 979 1110 21
Fragments arranged by size:
1110 979 149 21
SmlI C'TyrA_G
Cuts at: 0 1352 1767 2259
Size: 1352 415 492
Fragments arranged by size:
1352 492 415
StuI AGG'CCT
Cuts at: 0 1217 2158 2259
Size: 1217 941 101
Fragments arranged by size:
1217 941 101
StyI C'CwwG_G
Cuts at: 0 758 1033 1346 1806 1816 1898 2259
Size: 758 275 313 460 10 82 361
Fragments arranged by size:
758 460 361 313 275 82 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 34 2259
Size: 34 2225
TatI w'GTAC_w
Cuts at: 0 1871 2259
Size: 1871 388
Tth111I GACn'n_nGTC
Cuts at: 0 175 2259
Size: 175 2084
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 465 2259
Size: 465 1794
XmnI GAAnn'nnTTC
Cuts at: 0 2047 2259
Size: 2047 212
Enzymes that do cut:
AarI Acc65I AcuI AlwNI ApaLI ApoI AvrII BanI
BanII BbvCI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrFI BstAPI BstEII BstYI BtgI BtgZI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
HaeII Hin4I KpnI MfeI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NruI NspI PflMI PfoI
PpuMI PsiI PstI PvuII SacI SanDI SapI SfcI
SmlI StuI StyI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
BaeI BamHI BbeI BbsI BcgI BciVI BfrBI BmgBI
BmtI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EagI EcoNI FalI FseI
FspI FspAI HincII HindIII HpaI KasI MluI NarI
NheI NotI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XhoI XmaI ZraI