Restriction map for
pF1KE0046
(Linear) (Six-base) MAPSORT of: KIEE0046.seq Check: 1802 from: 1 to: 1917
KIEE0046.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1535 1917
Size: 1535 382
Acc65I G'GTAC_C
Cuts at: 0 734 1917
Size: 734 1183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 1185 1473 1917
Size: 46 1139 288 444
Fragments arranged by size:
1139 444 288 46
AfeI AGC'GCT
Cuts at: 0 1375 1917
Size: 1375 542
AflIII A'CryG_T
Cuts at: 0 1754 1917
Size: 1754 163
AhdI GACnn_n'nnGTC
Cuts at: 0 1119 1917
Size: 1119 798
AleI CACnn'nnGTG
Cuts at: 0 1261 1917
Size: 1261 656
AlwNI CAG_nnn'CTG
Cuts at: 0 647 1917
Size: 647 1270
ApaI G_GGCC'C
Cuts at: 0 231 1163 1917
Size: 231 932 754
Fragments arranged by size:
932 754 231
AvaI C'yCGr_G
Cuts at: 0 224 1615 1917
Size: 224 1391 302
Fragments arranged by size:
1391 302 224
BamHI G'GATC_C
Cuts at: 0 896 1917
Size: 896 1021
BanI G'GyrC_C
Cuts at: 0 623 734 846 1263 1555 1621 1714 1917
Size: 623 111 112 417 292 66 93 203
Fragments arranged by size:
623 417 292 203 112 111 93 66
BanII G_rGCy'C
Cuts at: 0 231 325 801 1163 1460 1871 1917
Size: 231 94 476 362 297 411 46
Fragments arranged by size:
476 411 362 297 231 94 46
BbeI G_GCGC'C
Cuts at: 0 1625 1917
Size: 1625 292
BbsI GAAGACnn'nnnn_
Cuts at: 0 582 1917
Size: 582 1335
BbvCI CC'TCA_GC
Cuts at: 0 16 1164 1917
Size: 16 1148 753
Fragments arranged by size:
1148 753 16
BglI GCCn_nnn'nGGC
Cuts at: 0 1634 1917
Size: 1634 283
BglII A'GATC_T
Cuts at: 0 384 1917
Size: 384 1533
Bme1580I G_kGCm'C
Cuts at: 0 77 231 1163 1917
Size: 77 154 932 754
Fragments arranged by size:
932 754 154 77
BmrI ACTGGGnnnn_n'
Cuts at: 0 524 1917
Size: 524 1393
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 936 1917
Size: 936 981
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 884 1663 1810 1917
Size: 478 406 779 147 107
Fragments arranged by size:
779 478 406 147 107
Bpu10I CC'TnA_GC
Cuts at: 0 16 1164 1917
Size: 16 1148 753
Fragments arranged by size:
1148 753 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 1917
Size: 680 1237
BsaBI GATnn'nnATC
Cuts at: 0 383 1917
Size: 383 1534
BsaHI Gr'CG_yC
Cuts at: 0 587 1622 1648 1917
Size: 587 1035 26 269
Fragments arranged by size:
1035 587 269 26
BsaWI w'CCGG_w
Cuts at: 0 751 893 1917
Size: 751 142 1024
Fragments arranged by size:
1024 751 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1222 1252 1741 1771 1917
Size: 1222 30 489 30 146
Fragments arranged by size:
1222 489 146 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 809 1045 1438 1917
Size: 809 236 393 479
Fragments arranged by size:
809 479 393 236
BseYI C'CCAG_C
Cuts at: 0 1481 1917
Size: 1481 436
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 1917
Size: 181 1736
BsiEI CG_ry'CG
Cuts at: 0 1271 1821 1917
Size: 1271 550 96
Fragments arranged by size:
1271 550 96
BsiHKAI G_wGCw'C
Cuts at: 0 581 1917
Size: 581 1336
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1639 1917
Size: 1639 278
Bsp1286I G_dGCh'C
Cuts at: 0 77 231 325 581 801 1163 1460 1871
Size: 77 154 94 256 220 362 297 411
Cuts at: 1871 1917
Size: 46
Fragments arranged by size:
411 362 297 256 220 154 94 77
46
BspHI T'CATG_A
Cuts at: 0 375 1917
Size: 375 1542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1535 1835 1917
Size: 1535 300 82
Fragments arranged by size:
1535 300 82
BsrFI r'CCGG_y
Cuts at: 0 1266 1726 1917
Size: 1266 460 191
Fragments arranged by size:
1266 460 191
BssHII G'CGCG_C
Cuts at: 0 1498 1592 1664 1917
Size: 1498 94 72 253
Fragments arranged by size:
1498 253 94 72
BssSI C'ACGA_G
Cuts at: 0 1345 1917
Size: 1345 572
BstEII G'GTnAC_C
Cuts at: 0 889 1917
Size: 889 1028
BstYI r'GATC_y
Cuts at: 0 384 614 896 1917
Size: 384 230 282 1021
Fragments arranged by size:
1021 384 282 230
BtgI C'CryG_G
Cuts at: 0 47 1155 1169 1256 1277 1476 1531 1629
Size: 47 1108 14 87 21 199 55 98
Cuts at: 1629 1800 1815 1917
Size: 171 15 102
Fragments arranged by size:
1108 199 171 102 98 87 55 47
21 15 14
BtsI GCAGTG_nn'
Cuts at: 0 169 1149 1917
Size: 169 980 768
Fragments arranged by size:
980 768 169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 824 859 1917
Size: 824 35 1058
Fragments arranged by size:
1058 824 35
DraIII CAC_nnn'GTG
Cuts at: 0 853 1917
Size: 853 1064
EaeI y'GGCC_r
Cuts at: 0 92 1268 1311 1818 1917
Size: 92 1176 43 507 99
Fragments arranged by size:
1176 507 99 92 43
EagI C'GGCC_G
Cuts at: 0 1268 1818 1917
Size: 1268 550 99
Fragments arranged by size:
1268 550 99
EarI CTCTTCn'nnn_
Cuts at: 0 37 106 601 1849 1917
Size: 37 69 495 1248 68
Fragments arranged by size:
1248 495 69 68 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1036 1917
Size: 1036 881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 478 884 1185 1473 1663 1810 1917
Size: 46 432 406 301 288 190 147 107
Fragments arranged by size:
432 406 301 288 190 147 107 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 791 1027 1917
Size: 791 236 890
Fragments arranged by size:
890 791 236
EcoO109I rG'GnC_Cy
Cuts at: 0 261 930 1160 1917
Size: 261 669 230 757
Fragments arranged by size:
757 669 261 230
HaeII r_GCGC'y
Cuts at: 0 1377 1605 1625 1688 1917
Size: 1377 228 20 63 229
Fragments arranged by size:
1377 229 228 63 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1105 1137 1222 1254 1636 1668 1741 1773
Size: 1105 32 85 32 382 32 73 32
Cuts at: 1773 1917
Size: 144
Fragments arranged by size:
1105 382 144 85 73 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 400 1917
Size: 400 1517
KasI G'GCGC_C
Cuts at: 0 1621 1917
Size: 1621 296
KpnI G_GTAC'C
Cuts at: 0 738 1917
Size: 738 1179
MluI A'CGCG_T
Cuts at: 0 1754 1917
Size: 1754 163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1208 1784 1917
Size: 1208 576 133
Fragments arranged by size:
1208 576 133
MscI TGG'CCA
Cuts at: 0 94 1917
Size: 94 1823
MslI CAynn'nnrTG
Cuts at: 0 817 1261 1917
Size: 817 444 656
Fragments arranged by size:
817 656 444
MspA1I CmG'CkG
Cuts at: 0 419 644 662 1157 1317 1397 1478 1533
Size: 419 225 18 495 160 80 81 55
Cuts at: 1533 1631 1802 1817 1917
Size: 98 171 15 100
Fragments arranged by size:
495 419 225 171 160 100 98 81
80 55 18 15
NaeI GCC'GGC
Cuts at: 0 1268 1728 1917
Size: 1268 460 189
Fragments arranged by size:
1268 460 189
NarI GG'CG_CC
Cuts at: 0 1622 1917
Size: 1622 295
NcoI C'CATG_G
Cuts at: 0 47 1169 1277 1917
Size: 47 1122 108 640
Fragments arranged by size:
1122 640 108 47
NgoMIV G'CCGG_C
Cuts at: 0 1266 1726 1917
Size: 1266 460 191
Fragments arranged by size:
1266 460 191
NotI GC'GGCC_GC
Cuts at: 0 1818 1917
Size: 1818 99
NspI r_CATG'y
Cuts at: 0 216 816 1917
Size: 216 600 1101
Fragments arranged by size:
1101 600 216
PasI CC'CwG_GG
Cuts at: 0 336 1917
Size: 336 1581
PpuMI rG'GwC_Cy
Cuts at: 0 261 930 1917
Size: 261 669 987
Fragments arranged by size:
987 669 261
PspOMI G'GGCC_C
Cuts at: 0 227 1159 1917
Size: 227 932 758
Fragments arranged by size:
932 758 227
PstI C_TGCA'G
Cuts at: 0 156 1917
Size: 156 1761
PvuII CAG'CTG
Cuts at: 0 644 1917
Size: 644 1273
SacII CC_GC'GG
Cuts at: 0 1158 1479 1534 1632 1803 1818 1917
Size: 1158 321 55 98 171 15 99
Fragments arranged by size:
1158 321 171 99 98 55 15
SanDI GG'GwC_CC
Cuts at: 0 261 1917
Size: 261 1656
SapI GCTCTTCn'nnn_
Cuts at: 0 106 1917
Size: 106 1811
ScaI AGT'ACT
Cuts at: 0 1429 1917
Size: 1429 488
SfcI C'TryA_G
Cuts at: 0 152 348 1779 1917
Size: 152 196 1431 138
Fragments arranged by size:
1431 196 152 138
SfoI GGC'GCC
Cuts at: 0 1623 1917
Size: 1623 294
SmaI CCC'GGG
Cuts at: 0 226 1917
Size: 226 1691
SmlI C'TyrA_G
Cuts at: 0 695 1917
Size: 695 1222
SphI G_CATG'C
Cuts at: 0 816 1917
Size: 816 1101
StuI AGG'CCT
Cuts at: 0 59 1917
Size: 59 1858
StyI C'CwwG_G
Cuts at: 0 47 206 842 1169 1277 1418 1917
Size: 47 159 636 327 108 141 499
Fragments arranged by size:
636 499 327 159 141 108 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 549 1480 1578 1917
Size: 549 931 98 339
Fragments arranged by size:
931 549 339 98
TatI w'GTAC_w
Cuts at: 0 935 1427 1917
Size: 935 492 490
Fragments arranged by size:
935 492 490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 537 1284 1917
Size: 537 747 633
Fragments arranged by size:
747 633 537
XmaI C'CCGG_G
Cuts at: 0 224 1917
Size: 224 1693
XmnI GAAnn'nnTTC
Cuts at: 0 59 1249 1917
Size: 59 1190 668
Fragments arranged by size:
1190 668 59
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AhdI AleI AlwNI
ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI BssHII BssSI
BstEII BstYI BtgI BtsI CspCI DraIII EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PpuMI PspOMI
PstI PvuII SacII SanDI SapI ScaI SfcI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AlfI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BclI BfrBI BlpI BmgBI BmtI BsaI BsaAI BsiWI
BsmI BspEI BsrBI BsrDI BsrGI BstAPI BstBI BstXI
BstZ17I Bsu36I BtgZI ClaI DraI DrdI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI MfeI
NdeI NheI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SbfI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TstI Tth111I XbaI
XhoI ZraI