Restriction map for
pF1KE0045
(Linear) (Six-base) MAPSORT of: KIEE0045.seq Check: 41 from: 1 to: 1983
KIEE0045.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 0 1619 1983
Size: 1619 364
Acc65I G'GTAC_C
Cuts at: 0 779 1983
Size: 779 1204
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 1625 1983
Size: 341 1284 358
Fragments arranged by size:
1284 358 341
AflII C'TTAA_G
Cuts at: 0 1974 1983
Size: 1974 9
AflIII A'CryG_T
Cuts at: 0 223 454 1983
Size: 223 231 1529
Fragments arranged by size:
1529 231 223
AleI CACnn'nnGTG
Cuts at: 0 1728 1983
Size: 1728 255
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 692 724 875 907 1983
Size: 692 32 151 32 1076
Fragments arranged by size:
1076 692 151 32 32
ApaLI G'TGCA_C
Cuts at: 0 1569 1983
Size: 1569 414
ApoI r'AATT_y
Cuts at: 0 326 851 1041 1983
Size: 326 525 190 942
Fragments arranged by size:
942 525 326 190
AseI AT'TA_AT
Cuts at: 0 652 1983
Size: 652 1331
BamHI G'GATC_C
Cuts at: 0 1252 1983
Size: 1252 731
BanI G'GyrC_C
Cuts at: 0 779 1983
Size: 779 1204
BbsI GAAGACnn'nnnn_
Cuts at: 0 210 413 577 1983
Size: 210 203 164 1406
Fragments arranged by size:
1406 210 203 164
BclI T'GATC_A
Cuts at: 0 1662 1983
Size: 1662 321
BfrBI ATG'CAT
Cuts at: 0 980 1087 1983
Size: 980 107 896
Fragments arranged by size:
980 896 107
Bme1580I G_kGCm'C
Cuts at: 0 1573 1983
Size: 1573 410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 395 581 1983
Size: 230 165 186 1402
Fragments arranged by size:
1402 230 186 165
BsaI GGTCTCn'nnnn_
Cuts at: 0 248 611 1983
Size: 248 363 1372
Fragments arranged by size:
1372 363 248
BsaAI yAC'GTr
Cuts at: 0 224 1983
Size: 224 1759
BsaBI GATnn'nnATC
Cuts at: 0 1859 1983
Size: 1859 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 692 722 875 905 954 984 1140 1170
Size: 692 30 153 30 49 30 156 30
Cuts at: 1170 1563 1593 1983
Size: 393 30 390
Fragments arranged by size:
692 393 390 156 153 49 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 592 815 1535 1568 1571 1865 1983
Size: 592 223 720 33 3 294 118
Fragments arranged by size:
720 592 294 223 118 33 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1055 1528 1983
Size: 1055 473 455
Fragments arranged by size:
1055 473 455
BsiHKAI G_wGCw'C
Cuts at: 0 640 1573 1983
Size: 640 933 410
Fragments arranged by size:
933 640 410
BsmI GAATG_Cn'
Cuts at: 0 1904 1983
Size: 1904 79
Bsp1286I G_dGCh'C
Cuts at: 0 640 1573 1983
Size: 640 933 410
Fragments arranged by size:
933 640 410
BspHI T'CATG_A
Cuts at: 0 723 1983
Size: 723 1260
BsrDI GCAATG_nn'
Cuts at: 0 1281 1909 1983
Size: 1281 628 74
Fragments arranged by size:
1281 628 74
BsrGI T'GTAC_A
Cuts at: 0 226 1983
Size: 226 1757
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1243 1983
Size: 1243 740
BstYI r'GATC_y
Cuts at: 0 745 920 1252 1681 1885 1983
Size: 745 175 332 429 204 98
Fragments arranged by size:
745 429 332 204 175 98
BtgI C'CryG_G
Cuts at: 0 924 1110 1983
Size: 924 186 873
Fragments arranged by size:
924 873 186
BtsI GCAGTG_nn'
Cuts at: 0 1516 1983
Size: 1516 467
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1799 1834 1983
Size: 1799 35 149
Fragments arranged by size:
1799 149 35
DraI TTT'AAA
Cuts at: 0 126 1983
Size: 126 1857
EarI CTCTTCn'nnn_
Cuts at: 0 56 206 645 1644 1983
Size: 56 150 439 999 339
Fragments arranged by size:
999 439 339 150 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 341 395 581 1625 1983
Size: 230 111 54 186 1044 358
Fragments arranged by size:
1044 358 230 186 111 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 403 435 593 625 954 986 1140 1172
Size: 403 32 158 32 329 32 154 32
Cuts at: 1172 1983
Size: 811
Fragments arranged by size:
811 403 329 158 154 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 129 1983
Size: 129 1854
KpnI G_GTAC'C
Cuts at: 0 783 1983
Size: 783 1200
MslI CAynn'nnrTG
Cuts at: 0 600 618 1086 1399 1728 1983
Size: 600 18 468 313 329 255
Fragments arranged by size:
600 468 329 313 255 18
MspA1I CmG'CkG
Cuts at: 0 559 1867 1983
Size: 559 1308 116
Fragments arranged by size:
1308 559 116
NcoI C'CATG_G
Cuts at: 0 924 1110 1983
Size: 924 186 873
Fragments arranged by size:
924 873 186
NdeI CA'TA_TG
Cuts at: 0 1083 1983
Size: 1083 900
NsiI A_TGCA'T
Cuts at: 0 982 1089 1983
Size: 982 107 894
Fragments arranged by size:
982 894 107
NspI r_CATG'y
Cuts at: 0 342 458 1091 1983
Size: 342 116 633 892
Fragments arranged by size:
892 633 342 116
PciI A'CATG_T
Cuts at: 0 454 1983
Size: 454 1529
PmlI CAC'GTG
Cuts at: 0 224 1983
Size: 224 1759
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 692 724 875 907 1983
Size: 692 32 151 32 1076
Fragments arranged by size:
1076 692 151 32 32
PvuII CAG'CTG
Cuts at: 0 559 1867 1983
Size: 559 1308 116
Fragments arranged by size:
1308 559 116
SapI GCTCTTCn'nnn_
Cuts at: 0 206 645 1644 1983
Size: 206 439 999 339
Fragments arranged by size:
999 439 339 206
SfcI C'TryA_G
Cuts at: 0 435 1983
Size: 435 1548
SmlI C'TyrA_G
Cuts at: 0 1974 1983
Size: 1974 9
SspI AAT'ATT
Cuts at: 0 112 1282 1983
Size: 112 1170 701
Fragments arranged by size:
1170 701 112
StuI AGG'CCT
Cuts at: 0 1693 1983
Size: 1693 290
StyI C'CwwG_G
Cuts at: 0 253 924 1110 1983
Size: 253 671 186 873
Fragments arranged by size:
873 671 253 186
TatI w'GTAC_w
Cuts at: 0 226 525 1360 1543 1983
Size: 226 299 835 183 440
Fragments arranged by size:
835 440 299 226 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1244 1276 1563 1595 1983
Size: 1244 32 287 32 388
Fragments arranged by size:
1244 388 287 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 395 486 505 890 1983
Size: 395 91 19 385 1093
Fragments arranged by size:
1093 395 385 91 19
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AleI AloI ApaLI
ApoI AseI BamHI BanI BbsI BclI BfrBI Bme1580I
BpmI BsaI BsaAI BsaBI BsaXI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BsrGI BstAPI BstYI BtgI
BtsI CspCI DraI EarI Eco57MI Hin4I HindIII KpnI
MslI MspA1I NcoI NdeI NsiI NspI PciI PmlI
PpiI PvuII SapI SfcI SmlI SspI StuI StyI
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AlfI AlwNI
ApaI AscI AsiSI AvaI AvrII BaeI BanII BbeI
BbvCI BcgI BciVI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaHI BsaWI BseYI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI MfeI MluI MmeI MscI NaeI NarI NgoMIV
NheI NotI NruI PacI PasI PflMI PfoI PmeI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
ZraI