Restriction map for
pF1KE0043
(Linear) (Six-base) MAPSORT of: KIEE0043.seq Check: 9962 from: 1 to: 1632
KIEE0043.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1385 1425 1632
Size: 718 667 40 207
Fragments arranged by size:
718 667 207 40
AflII C'TTAA_G
Cuts at: 0 993 1632
Size: 993 639
AflIII A'CryG_T
Cuts at: 0 128 1632
Size: 128 1504
AgeI A'CCGG_T
Cuts at: 0 814 1632
Size: 814 818
AlwNI CAG_nnn'CTG
Cuts at: 0 214 432 1317 1632
Size: 214 218 885 315
Fragments arranged by size:
885 315 218 214
ApaLI G'TGCA_C
Cuts at: 0 1506 1632
Size: 1506 126
ApoI r'AATT_y
Cuts at: 0 371 418 787 885 1423 1435 1493 1632
Size: 371 47 369 98 538 12 58 139
Fragments arranged by size:
538 371 369 139 98 58 47 12
AseI AT'TA_AT
Cuts at: 0 1573 1632
Size: 1573 59
AvaI C'yCGr_G
Cuts at: 0 252 1632
Size: 252 1380
BanII G_rGCy'C
Cuts at: 0 312 1632
Size: 312 1320
BbsI GAAGACnn'nnnn_
Cuts at: 0 58 171 331 857 1323 1632
Size: 58 113 160 526 466 309
Fragments arranged by size:
526 466 309 160 113 58
BbvCI CC'TCA_GC
Cuts at: 0 604 1632
Size: 604 1028
BclI T'GATC_A
Cuts at: 0 1604 1632
Size: 1604 28
BglII A'GATC_T
Cuts at: 0 62 1557 1632
Size: 62 1495 75
Fragments arranged by size:
1495 75 62
Bme1580I G_kGCm'C
Cuts at: 0 74 590 744 1510 1632
Size: 74 516 154 766 122
Fragments arranged by size:
766 516 154 122 74
BmgBI CAC'GTC
Cuts at: 0 129 1632
Size: 129 1503
BmrI ACTGGGnnnn_n'
Cuts at: 0 343 630 905 1632
Size: 343 287 275 727
Fragments arranged by size:
727 343 287 275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1564 1632
Size: 79 1485 68
Fragments arranged by size:
1485 79 68
Bpu10I CC'TnA_GC
Cuts at: 0 604 1632
Size: 604 1028
BsaWI w'CCGG_w
Cuts at: 0 814 1632
Size: 814 818
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 920 950 1632
Size: 920 30 682
Fragments arranged by size:
920 682 30
BseYI C'CCAG_C
Cuts at: 0 440 1632
Size: 440 1192
BsiHKAI G_wGCw'C
Cuts at: 0 1510 1632
Size: 1510 122
Bsp1286I G_dGCh'C
Cuts at: 0 74 312 590 744 1510 1632
Size: 74 238 278 154 766 122
Fragments arranged by size:
766 278 238 154 122 74
BsrFI r'CCGG_y
Cuts at: 0 814 1632
Size: 814 818
BssSI C'ACGA_G
Cuts at: 0 583 1632
Size: 583 1049
BstXI CCAn_nnnn'nTGG
Cuts at: 0 300 1632
Size: 300 1332
BstYI r'GATC_y
Cuts at: 0 62 491 659 1557 1632
Size: 62 429 168 898 75
Fragments arranged by size:
898 429 168 75 62
Bsu36I CC'TnA_GG
Cuts at: 0 319 1632
Size: 319 1313
BtgI C'CryG_G
Cuts at: 0 293 1632
Size: 293 1339
BtsI GCAGTG_nn'
Cuts at: 0 617 1632
Size: 617 1015
DraIII CAC_nnn'GTG
Cuts at: 0 1159 1632
Size: 1159 473
EarI CTCTTCn'nnn_
Cuts at: 0 234 565 1466 1632
Size: 234 331 901 166
Fragments arranged by size:
901 331 234 166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 718 1385 1425 1564 1632
Size: 79 639 667 40 139 68
Fragments arranged by size:
667 639 139 79 68 40
EcoO109I rG'GnC_Cy
Cuts at: 0 249 1314 1632
Size: 249 1065 318
Fragments arranged by size:
1065 318 249
EcoRI G'AATT_C
Cuts at: 0 371 1435 1632
Size: 371 1064 197
Fragments arranged by size:
1064 371 197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 1632
Size: 363 1269
HaeII r_GCGC'y
Cuts at: 0 119 1632
Size: 119 1513
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 211 243 1153 1185 1632
Size: 211 32 910 32 447
Fragments arranged by size:
910 447 211 32 32
HincII GTy'rAC
Cuts at: 0 555 1632
Size: 555 1077
HindIII A'AGCT_T
Cuts at: 0 7 797 1030 1632
Size: 7 790 233 602
Fragments arranged by size:
790 602 233 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 1632
Size: 695 937
MslI CAynn'nnrTG
Cuts at: 0 298 361 1632
Size: 298 63 1271
Fragments arranged by size:
1271 298 63
MspA1I CmG'CkG
Cuts at: 0 214 656 1632
Size: 214 442 976
Fragments arranged by size:
976 442 214
NcoI C'CATG_G
Cuts at: 0 293 1632
Size: 293 1339
PflMI CCAn_nnn'nTGG
Cuts at: 0 432 1632
Size: 432 1200
PfoI T'CCnGG_A
Cuts at: 0 938 1632
Size: 938 694
PpuMI rG'GwC_Cy
Cuts at: 0 249 1632
Size: 249 1383
PstI C_TGCA'G
Cuts at: 0 219 1632
Size: 219 1413
PvuII CAG'CTG
Cuts at: 0 214 656 1632
Size: 214 442 976
Fragments arranged by size:
976 442 214
SapI GCTCTTCn'nnn_
Cuts at: 0 1466 1632
Size: 1466 166
SexAI A'CCwGG_T
Cuts at: 0 245 399 431 1632
Size: 245 154 32 1201
Fragments arranged by size:
1201 245 154 32
SfcI C'TryA_G
Cuts at: 0 215 1632
Size: 215 1417
SgrAI Cr'CCGG_yG
Cuts at: 0 814 1632
Size: 814 818
SmaI CCC'GGG
Cuts at: 0 254 1632
Size: 254 1378
SmlI C'TyrA_G
Cuts at: 0 993 1632
Size: 993 639
SspI AAT'ATT
Cuts at: 0 1053 1577 1632
Size: 1053 524 55
Fragments arranged by size:
1053 524 55
StyI C'CwwG_G
Cuts at: 0 293 570 1632
Size: 293 277 1062
Fragments arranged by size:
1062 293 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 320 612 924 991 1632
Size: 320 292 312 67 641
Fragments arranged by size:
641 320 312 292 67
TatI w'GTAC_w
Cuts at: 0 135 330 1632
Size: 135 195 1302
Fragments arranged by size:
1302 195 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 281 313 610 642 1632
Size: 281 32 297 32 990
Fragments arranged by size:
990 297 281 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 247 1342 1632
Size: 247 1095 290
Fragments arranged by size:
1095 290 247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 496 1632
Size: 496 1136
XmaI C'CCGG_G
Cuts at: 0 252 1632
Size: 252 1380
XmnI GAAnn'nnTTC
Cuts at: 0 8 1475 1632
Size: 8 1467 157
Fragments arranged by size:
1467 157 8
Enzymes that do cut:
AcuI AflII AflIII AgeI AlwNI ApaLI ApoI AseI
AvaI BanII BbsI BbvCI BclI BglII Bme1580I BmgBI
BmrI BpmI Bpu10I BsaWI BsaXI BseYI BsiHKAI Bsp1286I
BsrFI BssSI BstXI BstYI Bsu36I BtgI BtsI DraIII
EarI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII MmeI MslI MspA1I NcoI PflMI PfoI PpuMI
PstI PvuII SapI SexAI SfcI SgrAI SmaI SmlI
SspI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AhdI AleI
AlfI AloI ApaI AscI AsiSI AvrII BaeI BamHI
BanI BbeI BcgI BciVI BfrBI BglI BlpI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BseRI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRV FseI FspI FspAI HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SnaBI SpeI SphI SrfI StuI SwaI
XbaI XhoI ZraI