Restriction map for
pF1KE0035
(Linear) (Six-base) MAPSORT of: KIEE0035.seq Check: 2414 from: 1 to: 1008
KIEE0035.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 1008
Size: 479 529
AlwNI CAG_nnn'CTG
Cuts at: 0 93 301 1008
Size: 93 208 707
Fragments arranged by size:
707 208 93
ApaI G_GGCC'C
Cuts at: 0 80 1008
Size: 80 928
AvrII C'CTAG_G
Cuts at: 0 400 1008
Size: 400 608
BanI G'GyrC_C
Cuts at: 0 844 1008
Size: 844 164
BanII G_rGCy'C
Cuts at: 0 80 272 326 1008
Size: 80 192 54 682
Fragments arranged by size:
682 192 80 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 136 460 1008
Size: 136 324 548
Fragments arranged by size:
548 324 136
BclI T'GATC_A
Cuts at: 0 140 209 416 1008
Size: 140 69 207 592
Fragments arranged by size:
592 207 140 69
BglI GCCn_nnn'nGGC
Cuts at: 0 16 244 1008
Size: 16 228 764
Fragments arranged by size:
764 228 16
Bme1580I G_kGCm'C
Cuts at: 0 80 445 503 849 1008
Size: 80 365 58 346 159
Fragments arranged by size:
365 346 159 80 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 818 1008
Size: 373 445 190
Fragments arranged by size:
445 373 190
BsaHI Gr'CG_yC
Cuts at: 0 305 1008
Size: 305 703
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 191 221 1008
Size: 191 30 787
Fragments arranged by size:
787 191 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 523 1008
Size: 256 267 485
Fragments arranged by size:
485 267 256
BseYI C'CCAG_C
Cuts at: 0 550 1008
Size: 550 458
BsiEI CG_ry'CG
Cuts at: 0 179 1008
Size: 179 829
BsiHKAI G_wGCw'C
Cuts at: 0 272 326 1008
Size: 272 54 682
Fragments arranged by size:
682 272 54
Bsp1286I G_dGCh'C
Cuts at: 0 80 272 326 445 503 849 1008
Size: 80 192 54 119 58 346 159
Fragments arranged by size:
346 192 159 119 80 58 54
BsrBI CCG'CTC
Cuts at: 0 34 1008
Size: 34 974
BsrFI r'CCGG_y
Cuts at: 0 65 1008
Size: 65 943
BssHII G'CGCG_C
Cuts at: 0 230 232 234 366 1008
Size: 230 2 2 132 642
Fragments arranged by size:
642 230 132 2 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 864 945 1008
Size: 864 81 63
Fragments arranged by size:
864 81 63
BstYI r'GATC_y
Cuts at: 0 431 1008
Size: 431 577
Bsu36I CC'TnA_GG
Cuts at: 0 878 1008
Size: 878 130
BtgI C'CryG_G
Cuts at: 0 173 281 1008
Size: 173 108 727
Fragments arranged by size:
727 173 108
BtsI GCAGTG_nn'
Cuts at: 0 11 1008
Size: 11 997
EaeI y'GGCC_r
Cuts at: 0 8 29 176 1008
Size: 8 21 147 832
Fragments arranged by size:
832 147 21 8
EagI C'GGCC_G
Cuts at: 0 176 1008
Size: 176 832
EarI CTCTTCn'nnn_
Cuts at: 0 389 805 876 1008
Size: 389 416 71 132
Fragments arranged by size:
416 389 132 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 250 262 1008
Size: 250 12 746
Fragments arranged by size:
746 250 12
EcoICRI GAG'CTC
Cuts at: 0 270 324 1008
Size: 270 54 684
Fragments arranged by size:
684 270 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 479 818 1008
Size: 373 106 339 190
Fragments arranged by size:
373 339 190 106
EcoO109I rG'GnC_Cy
Cuts at: 0 76 108 1008
Size: 76 32 900
Fragments arranged by size:
900 76 32
EcoRV GAT'ATC
Cuts at: 0 978 1008
Size: 978 30
FspI TGC'GCA
Cuts at: 0 97 1008
Size: 97 911
HaeII r_GCGC'y
Cuts at: 0 49 1008
Size: 49 959
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 1008
Size: 334 32 642
Fragments arranged by size:
642 334 32
HindIII A'AGCT_T
Cuts at: 0 639 1008
Size: 639 369
MscI TGG'CCA
Cuts at: 0 10 1008
Size: 10 998
MspA1I CmG'CkG
Cuts at: 0 90 158 283 311 1008
Size: 90 68 125 28 697
Fragments arranged by size:
697 125 90 68 28
PasI CC'CwG_GG
Cuts at: 0 80 1008
Size: 80 928
PflMI CCAn_nnn'nTGG
Cuts at: 0 301 1008
Size: 301 707
PfoI T'CCnGG_A
Cuts at: 0 332 389 1008
Size: 332 57 619
Fragments arranged by size:
619 332 57
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 1008
Size: 463 32 513
Fragments arranged by size:
513 463 32
PpuMI rG'GwC_Cy
Cuts at: 0 108 1008
Size: 108 900
PsiI TTA'TAA
Cuts at: 0 584 1008
Size: 584 424
PspOMI G'GGCC_C
Cuts at: 0 76 1008
Size: 76 932
SacI G_AGCT'C
Cuts at: 0 272 326 1008
Size: 272 54 682
Fragments arranged by size:
682 272 54
SacII CC_GC'GG
Cuts at: 0 284 1008
Size: 284 724
SanDI GG'GwC_CC
Cuts at: 0 108 1008
Size: 108 900
SapI GCTCTTCn'nnn_
Cuts at: 0 389 1008
Size: 389 619
ScaI AGT'ACT
Cuts at: 0 26 1008
Size: 26 982
StyI C'CwwG_G
Cuts at: 0 400 525 1008
Size: 400 125 483
Fragments arranged by size:
483 400 125
TatI w'GTAC_w
Cuts at: 0 24 1008
Size: 24 984
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 191 223 1008
Size: 191 32 785
Fragments arranged by size:
785 191 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 558 1008
Size: 558 450
Enzymes that do cut:
AcuI AlwNI ApaI AvrII BanI BanII BbsI BclI
BglI Bme1580I BpmI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BsrBI BsrFI BssHII BstXI BstYI Bsu36I
BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRV FspI HaeII Hin4I HindIII MscI MspA1I
PasI PflMI PfoI PpiI PpuMI PsiI PspOMI SacI
SacII SanDI SapI ScaI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvaI BaeI BamHI BbeI BbvCI BcgI
BciVI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EcoNI EcoRI FalI FseI FspAI HincII
HpaI KasI KpnI MfeI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PshAI PspXI PsrI
PstI PvuI PvuII RsrII SalI SbfI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XhoI XmaI XmnI ZraI