Restriction map for
pF1KE0033
(Linear) (Six-base) MAPSORT of: KIEE0033.seq Check: 785 from: 1 to: 1692
KIEE0033.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1650 1692
Size: 1650 42
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 1692
Size: 218 1474
AfeI AGC'GCT
Cuts at: 0 54 1692
Size: 54 1638
AflIII A'CryG_T
Cuts at: 0 1671 1692
Size: 1671 21
AleI CACnn'nnGTG
Cuts at: 0 73 1692
Size: 73 1619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 509 1692
Size: 509 1183
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1034 1066 1692
Size: 1034 32 626
Fragments arranged by size:
1034 626 32
ApaI G_GGCC'C
Cuts at: 0 1235 1692
Size: 1235 457
ApoI r'AATT_y
Cuts at: 0 410 739 1132 1423 1692
Size: 410 329 393 291 269
Fragments arranged by size:
410 393 329 291 269
AseI AT'TA_AT
Cuts at: 0 1256 1692
Size: 1256 436
AvaI C'yCGr_G
Cuts at: 0 1622 1692
Size: 1622 70
BanII G_rGCy'C
Cuts at: 0 313 1235 1623 1692
Size: 313 922 388 69
Fragments arranged by size:
922 388 313 69
BglI GCCn_nnn'nGGC
Cuts at: 0 64 1692
Size: 64 1628
BglII A'GATC_T
Cuts at: 0 641 1692
Size: 641 1051
BlpI GC'TnA_GC
Cuts at: 0 49 971 1692
Size: 49 922 721
Fragments arranged by size:
922 721 49
Bme1580I G_kGCm'C
Cuts at: 0 1235 1692
Size: 1235 457
BmrI ACTGGGnnnn_n'
Cuts at: 0 714 1293 1692
Size: 714 579 399
Fragments arranged by size:
714 579 399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1094 1692
Size: 1094 598
Bpu10I CC'TnA_GC
Cuts at: 0 1595 1692
Size: 1595 97
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 353 383 1034 1064 1442 1472 1692
Size: 353 30 651 30 378 30 220
Fragments arranged by size:
651 378 353 220 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1458 1692
Size: 1458 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1522 1692
Size: 1522 170
BsmI GAATG_Cn'
Cuts at: 0 634 1017 1692
Size: 634 383 675
Fragments arranged by size:
675 634 383
Bsp1286I G_dGCh'C
Cuts at: 0 313 1235 1623 1692
Size: 313 922 388 69
Fragments arranged by size:
922 388 313 69
BspHI T'CATG_A
Cuts at: 0 723 1692
Size: 723 969
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1650 1692
Size: 1650 42
BsrDI GCAATG_nn'
Cuts at: 0 1439 1692
Size: 1439 253
BsrGI T'GTAC_A
Cuts at: 0 153 352 1218 1692
Size: 153 199 866 474
Fragments arranged by size:
866 474 199 153
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1273 1509 1692
Size: 1273 236 183
Fragments arranged by size:
1273 236 183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 75 1692
Size: 75 1617
BstYI r'GATC_y
Cuts at: 0 534 641 1589 1692
Size: 534 107 948 103
Fragments arranged by size:
948 534 107 103
BtsI GCAGTG_nn'
Cuts at: 0 20 1043 1692
Size: 20 1023 649
Fragments arranged by size:
1023 649 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1224 1259 1692
Size: 1224 35 433
Fragments arranged by size:
1224 433 35
DraI TTT'AAA
Cuts at: 0 218 1692
Size: 218 1474
DrdI GACnn_nn'nnGTC
Cuts at: 0 1359 1692
Size: 1359 333
EaeI y'GGCC_r
Cuts at: 0 65 1692
Size: 65 1627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 1094 1692
Size: 218 876 598
Fragments arranged by size:
876 598 218
EcoRI G'AATT_C
Cuts at: 0 410 1692
Size: 410 1282
EcoRV GAT'ATC
Cuts at: 0 1124 1692
Size: 1124 568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 534 537 1292 1692
Size: 534 3 755 400
Fragments arranged by size:
755 534 400 3
HaeII r_GCGC'y
Cuts at: 0 56 1692
Size: 56 1636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 1392 1692
Size: 144 1248 300
Fragments arranged by size:
1248 300 144
MslI CAynn'nnrTG
Cuts at: 0 73 1692
Size: 73 1619
MspA1I CmG'CkG
Cuts at: 0 48 1692
Size: 48 1644
NspI r_CATG'y
Cuts at: 0 1675 1692
Size: 1675 17
PciI A'CATG_T
Cuts at: 0 1671 1692
Size: 1671 21
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1034 1066 1692
Size: 1034 32 626
Fragments arranged by size:
1034 626 32
PspOMI G'GGCC_C
Cuts at: 0 1231 1692
Size: 1231 461
PstI C_TGCA'G
Cuts at: 0 97 1692
Size: 97 1595
PvuII CAG'CTG
Cuts at: 0 48 1692
Size: 48 1644
SfcI C'TryA_G
Cuts at: 0 93 753 1692
Size: 93 660 939
Fragments arranged by size:
939 660 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 725 1692
Size: 725 967
TatI w'GTAC_w
Cuts at: 0 153 352 1218 1692
Size: 153 199 866 474
Fragments arranged by size:
866 474 199 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 411 443 603 635 1692
Size: 411 32 160 32 1057
Fragments arranged by size:
1057 411 160 32 32
XbaI T'CTAG_A
Cuts at: 0 766 1471 1692
Size: 766 705 221
Fragments arranged by size:
766 705 221
XmnI GAAnn'nnTTC
Cuts at: 0 517 1049 1692
Size: 517 532 643
Fragments arranged by size:
643 532 517
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AloI ApaI
ApoI AseI AvaI BanII BglI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BsaXI BseRI BsgI BsmI Bsp1286I
BspHI BspMI BsrDI BsrGI BstAPI BstXI BstYI BtsI
CspCI DraI DrdI EaeI Eco57MI EcoRI EcoRV FalI
HaeII MmeI MslI MspA1I NspI PciI PpiI PspOMI
PstI PvuII SfcI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AlwNI
ApaLI AscI AsiSI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI
DraIII EagI EarI EciI EcoICRI EcoNI EcoO109I FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI Tth111I
XcmI XhoI XmaI ZraI