Restriction map for
pF1KE0031
(Linear) (Six-base) MAPSORT of: KIEE0031.seq Check: 8504 from: 1 to: 1482
KIEE0031.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 0 1061 1482
Size: 1061 421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1419 1482
Size: 1419 63
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1264 1296 1482
Size: 1264 32 186
Fragments arranged by size:
1264 186 32
ApoI r'AATT_y
Cuts at: 0 508 1461 1482
Size: 508 953 21
Fragments arranged by size:
953 508 21
BanII G_rGCy'C
Cuts at: 0 366 1005 1482
Size: 366 639 477
Fragments arranged by size:
639 477 366
BbsI GAAGACnn'nnnn_
Cuts at: 0 950 1482
Size: 950 532
BbvCI CC'TCA_GC
Cuts at: 0 1253 1482
Size: 1253 229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 635 669 1482
Size: 635 34 813
Fragments arranged by size:
813 635 34
BfrBI ATG'CAT
Cuts at: 0 109 1482
Size: 109 1373
Bme1580I G_kGCm'C
Cuts at: 0 504 1482
Size: 504 978
BmtI G_CTAG'C
Cuts at: 0 188 1482
Size: 188 1294
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1019 1482
Size: 1019 463
Bpu10I CC'TnA_GC
Cuts at: 0 389 919 1253 1482
Size: 389 530 334 229
Fragments arranged by size:
530 389 334 229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1014 1482
Size: 526 488 468
Fragments arranged by size:
526 488 468
BsaBI GATnn'nnATC
Cuts at: 0 1388 1482
Size: 1388 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1264 1294 1482
Size: 1264 30 188
Fragments arranged by size:
1264 188 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1282 1285 1378 1482
Size: 1282 3 93 104
Fragments arranged by size:
1282 104 93 3
BseYI C'CCAG_C
Cuts at: 0 564 1482
Size: 564 918
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 933 1482
Size: 933 549
BsiEI CG_ry'CG
Cuts at: 0 41 1482
Size: 41 1441
BsiHKAI G_wGCw'C
Cuts at: 0 1005 1482
Size: 1005 477
BsmI GAATG_Cn'
Cuts at: 0 336 417 1176 1411 1482
Size: 336 81 759 235 71
Fragments arranged by size:
759 336 235 81 71
Bsp1286I G_dGCh'C
Cuts at: 0 366 504 1005 1482
Size: 366 138 501 477
Fragments arranged by size:
501 477 366 138
BsrDI GCAATG_nn'
Cuts at: 0 566 740 1482
Size: 566 174 742
Fragments arranged by size:
742 566 174
BsrGI T'GTAC_A
Cuts at: 0 833 1482
Size: 833 649
BssSI C'ACGA_G
Cuts at: 0 727 1482
Size: 727 755
BstAPI GCAn_nnn'nTGC
Cuts at: 0 115 746 1482
Size: 115 631 736
Fragments arranged by size:
736 631 115
BstZ17I GTA'TAC
Cuts at: 0 1062 1482
Size: 1062 420
BtgI C'CryG_G
Cuts at: 0 11 335 1482
Size: 11 324 1147
Fragments arranged by size:
1147 324 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 35 511 1482
Size: 35 476 971
Fragments arranged by size:
971 476 35
BtsI GCAGTG_nn'
Cuts at: 0 965 1482
Size: 965 517
DrdI GACnn_nn'nnGTC
Cuts at: 0 283 1482
Size: 283 1199
EarI CTCTTCn'nnn_
Cuts at: 0 67 993 1120 1482
Size: 67 926 127 362
Fragments arranged by size:
926 362 127 67
EcoICRI GAG'CTC
Cuts at: 0 1003 1482
Size: 1003 479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1419 1482
Size: 1419 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1360 1482
Size: 1360 122
EcoO109I rG'GnC_Cy
Cuts at: 0 1353 1482
Size: 1353 129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1431 1482
Size: 1431 51
FspI TGC'GCA
Cuts at: 0 1109 1482
Size: 1109 373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1209 1241 1482
Size: 1209 32 241
Fragments arranged by size:
1209 241 32
HindIII A'AGCT_T
Cuts at: 0 137 916 964 1196 1482
Size: 137 779 48 232 286
Fragments arranged by size:
779 286 232 137 48
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 1272 1482
Size: 251 1021 210
Fragments arranged by size:
1021 251 210
MslI CAynn'nnrTG
Cuts at: 0 166 214 383 1375 1482
Size: 166 48 169 992 107
Fragments arranged by size:
992 169 166 107 48
MspA1I CmG'CkG
Cuts at: 0 13 1113 1482
Size: 13 1100 369
Fragments arranged by size:
1100 369 13
NcoI C'CATG_G
Cuts at: 0 335 1482
Size: 335 1147
NdeI CA'TA_TG
Cuts at: 0 825 1482
Size: 825 657
NheI G'CTAG_C
Cuts at: 0 184 1482
Size: 184 1298
NsiI A_TGCA'T
Cuts at: 0 111 1482
Size: 111 1371
NspI r_CATG'y
Cuts at: 0 347 1482
Size: 347 1135
PasI CC'CwG_GG
Cuts at: 0 846 1482
Size: 846 636
PfoI T'CCnGG_A
Cuts at: 0 233 1482
Size: 233 1249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1264 1296 1482
Size: 1264 32 186
Fragments arranged by size:
1264 186 32
PpuMI rG'GwC_Cy
Cuts at: 0 1353 1482
Size: 1353 129
PsiI TTA'TAA
Cuts at: 0 143 1482
Size: 143 1339
PstI C_TGCA'G
Cuts at: 0 751 1482
Size: 751 731
PvuII CAG'CTG
Cuts at: 0 1113 1482
Size: 1113 369
SacI G_AGCT'C
Cuts at: 0 1005 1482
Size: 1005 477
SacII CC_GC'GG
Cuts at: 0 14 1482
Size: 14 1468
SapI GCTCTTCn'nnn_
Cuts at: 0 993 1482
Size: 993 489
SfcI C'TryA_G
Cuts at: 0 747 1482
Size: 747 735
SmlI C'TyrA_G
Cuts at: 0 541 993 1482
Size: 541 452 489
Fragments arranged by size:
541 489 452
StuI AGG'CCT
Cuts at: 0 736 1300 1482
Size: 736 564 182
Fragments arranged by size:
736 564 182
StyI C'CwwG_G
Cuts at: 0 335 1482
Size: 335 1147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 1369 1482
Size: 55 1314 113
Fragments arranged by size:
1314 113 55
TatI w'GTAC_w
Cuts at: 0 833 1482
Size: 833 649
XmnI GAAnn'nnTTC
Cuts at: 0 615 1279 1482
Size: 615 664 203
Fragments arranged by size:
664 615 203
Enzymes that do cut:
AccI AcuI AloI ApoI BanII BbsI BbvCI BcgI
BfrBI Bme1580I BmtI BplI Bpu10I BpuEI BsaBI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrDI
BsrGI BssSI BstAPI BstZ17I BtgI BtgZI BtsI DrdI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI Hin4I
HindIII MmeI MslI MspA1I NcoI NdeI NheI NsiI
NspI PasI PfoI PpiI PpuMI PsiI PstI PvuII
SacI SacII SapI SfcI SmlI StuI StyI TaqII
TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BciVI
BclI BglI BglII BlpI BmgBI BmrI BpmI BsaI
BsaAI BsaHI BsaWI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BssHII BstBI BstEII BstXI BstYI Bsu36I
ClaI CspCI DraI DraIII EaeI EagI EciI EcoRI
EcoRV FseI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NgoMIV NotI NruI
PacI PciI PflMI PmeI PmlI PshAI PspOMI PspXI
PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI